人白介素-13對牛主動脈內皮細胞表達黏附分子的影響_《中國普外基礎與臨床雜志》

作者：

陳剛 ¹ , 傅志仁 ¹ , 王梁華 ² , 焦炳華 ²

1.第二軍醫大學長征醫院器官移植中心（上海200003）;;
2.第二軍醫大學基礎部生化與分子生物學系（上海200433）;

關鍵詞：

人白介素-13 牛主動脈內皮細胞細胞間黏附分子-1 E-選擇素

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摘要】目的研究人白介素-13（hIL-13）對牛主動脈內皮細胞（BAECs）表達細胞間黏附分子-1（ICAM-1）和E-選擇素的影響，為hIL-13應用于誘導異種移植免疫耐受、減輕異種移植排斥反應提供實驗依據。
方法用不同濃度的hIL-13（2、5、10、20及40 ng/ml）孵育 BAECs 2 h后，再與腫瘤壞死因子-α（TNF-α， 4 ng/ml）共孵育6 h或18 h；應用細胞酶聯免疫吸附分析方法(Cell-ELISA)檢測BAECs表面的E-選擇素和ICAM-1的表達；用MTT方法測定hIL-13對BAECs活性的影響。
結果用hIL-13在5~20 ng/ml濃度內預處理BAECs后，能明顯抑制TNF-α誘導的E-選擇素與ICAM-1的表達，并呈現一定的劑量依賴性(P＜0.01)。經不同濃度hIL-13處理的BAECs活性與未經hIL-13處理者相比差異無統計學意義。
結論 hIL-13能明顯抑制BAECs表達E-選擇素與ICAM-1，誘導異種移植的免疫耐受，且hIL-13對BAECs無損傷作用。

引用本文： 陳剛,傅志仁,王梁華,焦炳華. 人白介素-13對牛主動脈內皮細胞表達黏附分子的影響. 中國普外基礎與臨床雜志, 2005, 12(5): 469-472. doi: 復制

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1. Macarak EJ, Howard BV, Kefalides NA. Properties of calf endothelial cells in culture [Ｊ]. Lab Invest， 1977; 36(1)∶62.
2. Minter AJ, Keoshkerian E, Chesterman CN, et al. Fibroblast growth factor and heparin protect endothelial cells from the effects of interleukin 1 [Ｊ]. J Cell Physiol, 1996; 167(2)∶229.
3. Villarete LH, Remick DG. Nitric oxide regulation of IL8 expression in human endothelial cells [Ｊ]. Biochem Biophys Res Commun, 1995; 211(2)∶671.
4. Weigel G, Grimm M, Griesmacher A, et al. Adhesion molecule behavior during rejection and infection episodes after heart transplantation [Ｊ]. Clin Chem Lab Med, 2000; 38(5)∶403.
5. Yamazaki S, Isobe M, Suzuki J, et al. Role of selectindependent adhesion in cardiac allograft rejection [Ｊ]. J Heart Lung Transplant, 1998; 17(10)∶1007.
6. Satoh S, Thomson AW, Nakamura K, et al. Circulating ICAM1, Eselectin, IL2 receptor, and HLA class I in human small bowel, liver, and small bowelplusliver transplant recipients [Ｊ]. Transplantation, 1995; 60(6)∶558.
7. Nowaczyk M, Gorski A, Durlik M, et al. Role of adhesion molecules in chronic allograft rejection [Ｊ]. Arch Immunol Ther Exp (Warsz), 1999; 47(6)∶373.
8. Burke GW, Ciancio G, Cirocco R, et al. Association of interleukin10 with rejectionsparing effect in septic kidney transplant recipients [Ｊ]. Transplantation, 1996; 61(7)∶1114.
9. Ishido N, Matsuoka J, Matsuno T, et al. Induction of donorspecific hyporesponsiveness and prolongation of cardiac allograft survival by jejunal administration of donor splenocytes [Ｊ]. Transplantation, 1999; 68(9)∶1377.
10. Chen CC, Rosenbloom CL, Anderson DC, et al. Selective inhibition of Eselectin, vascular cell adhesion molecule1, and intercellular adhesion molecule1 expression by inhibitors of I kappa Balpha phosphorylation [Ｊ]. J Immunol, 1995; 155(7)∶3538.
11. Montgomery KF, Osborn L, Hession C, et al. Activation of endothelialleukocyte adhesion molecule 1 (ELAM1) gene transcription [Ｊ]. Proc Natl Acad Sci USA, 1991; 88(15)∶6523.
12. Hou J, Baichwal V, Cao Z. Regulatory elements and transcription factors controlling basal and cytokineinduced expression of the gene encoding intercellular adhesion molecule 1 [Ｊ]. Proc Natl Acad Sci USA, 1994； 91(24)∶11641.
13. Martin PL, Davis D, Weiss M, et al. Xenotransplantation of bovine bone marrow stromal cells into pig fetuses: incorporation into skeletal muscle [Ｊ]. Anim Biotechnol, 2001; 12(2)∶183.
14. Yoo JG, Choe SY, Rho GJ. Efficient production of cloned bovine embryos using cdc2 kinase inhibitor [Ｊ]. Reprod Domest Anim, 2003; 38(6)∶444.
15. Lentsch AB, Shanley TP, Sarma V, et al. In vivo suppression of NFkappa B and preservation of I kappa B alpha by interleukin10 and interleukin13 [Ｊ]. J Clin Invest, 1997; 100(10)∶2443.

《中國普外基礎與臨床雜志》

人白介素-13對牛主動脈內皮細胞表達黏附分子的影響

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《中國普外基礎與臨床雜志》

人白介素-13對牛主動脈內皮細胞表達黏附分子的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料