切應力對大鼠肝竇內皮細胞分泌功能的影響_《中國普外基礎與臨床雜志》

作者：

丁紀明 , 陳平 , 張林 , 朱瑾 , 熊燕 , 段世剛 , 李穎

第三軍醫大學西南醫院全軍肝膽外科研究所(重慶400038);;
通訊作者：陳平;

關鍵詞：

切應力肝竇內皮細胞分泌功能細胞因子

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

【摘要】目的了解不同切應力下肝竇內皮細胞分泌肝細胞生長因子（HGF）、白細胞介素-6（IL-6）、一氧化氮（NO）及一氧化氮合成酶（NOS）的分泌量，探討切應力對原代培養的大鼠肝竇內皮細胞分泌功能的影響。
方法構建血流動力學模型，實驗分為對照組（切應力為0 dyn/cm2）和實驗組2組,實驗組又按切應力不同分為12、24和 48 dyn/cm2 3個亞組。每組分別在4、8、12及24 h抽取1 ml培養基，利用試劑盒檢測培養基中HGF、IL-6、NO和NOS濃度。
結果在不同切應力下,肝竇內皮細胞分泌HGF、IL-6、NO和NOS各不相同，在同一時相隨著切應力的升高,HGF、IL-6、NO和NOS的分泌量也升高，實驗組明顯高于對照組(P＜0.01），且48 dyn/cm2組明顯高于另兩個亞組(P＜0.01）；實驗組作用8、12、24 h時，HGF、IL-6、No及NOS分泌量明顯高于作用4 h時。
結論體外實驗證實,切應力升高時肝竇內皮細胞分泌HGF、IL-6、NO和NOS的量明顯增加,提示部分肝切除術后門靜脈壓力急劇升高能促使肝竇內皮細胞活化，分泌大量促肝細胞再生的細胞因子和介質。

引用本文： 丁紀明,陳平,張林,朱瑾,熊燕,段世剛,李穎. 切應力對大鼠肝竇內皮細胞分泌功能的影響. 中國普外基礎與臨床雜志, 2005, 12(5): 455-458下轉468. doi: 復制

1.	Kahn D, van HoornHickman R, Terblanche J. Liver blood flow after partial hepatectomy in the pig [Ｊ]. J Surg Res, 1984; 37(4)∶290.
2.	LeCouter J, Moritz DR, Li B, et al. Angiogenesisindependent endothelial protection of liver: role of VEGFR1 [Ｊ]. Science, 2003; 299(5608)∶890.
3.	Xu B, Broome U, Uzunel M, et al. Capillarization of hepatic sinusoid by liver endothelial cellreactive autoantibodies in patients with cirrhosis and chronic hepatitis [Ｊ]. Am J Pathol, 2003; 163(4)∶1275.
4.	Mendoza L, Carrascal T, De Luca M, et al. Hydrogen peroxide mediates vascular cell adhesion molecule1 expression from interleukin18activated hepatic sinusoidal endothelium: implications for circulating cancer cell arrest in the murine liver [Ｊ]. Hepatology, 2001; 34(2)∶298.
5.	朱瑾, 陳平,熊燕, 等. 大鼠肝竇內皮細胞分離、培養及其生物學特性的初步研究 [Ｊ]. 消化外科， 2004； 3（5）∶350.
6.	Huet PM, Nagaoka MR, Desbiens G, et al. Sinusoidal endothelial cell and hepatocyte death following cold ischemiawarm reperfusion of the rat liver [Ｊ]. Hepatology, 2004; 39(4)∶1110.
7.	Nedredal GI, Elvevold KH, Ytrebo LM, et al. Liver sinusoidal endothelial cells represents an important blood clearance system in pigs [Ｊ]. Comp Hepatol, 2003; 2(1)∶1.
8.	Limmer A, Knolle PA. Liver sinusoidal endothelial cells: a new type of organresident antigenpresenting cell [Ｊ]. Arch Immunol Ther Exp (Warsz), 2001; 49(Suppl 1)∶S7.
9.	Mendoza L, VidalVanaclocha F. Inflammatory response of tumoractivated hepatic sinusoidal endothelium as a target for the screening of metastasis chemopreventive drugs [Ｊ]. Methods Mol Med, 2003; 85∶107.
10.	Thomson RY, Clarke AM. Role of portal blood supply in liver regeneration [Ｊ]. Nature, 1965; 208(8)∶392.
11.	Schoen JM, Wang HH, Minuk GY, et al. Shear stressinduced nitric oxide release triggers the liver regeneration cascade [Ｊ]. Nitric Oxide, 2001; 5(5)∶453.
12.	Kaneko T, Sugimoto H, Inoue S, et al. Doppler ultrasonography for postoperative hepatofugal portal blood flow of the intrahepatic portal vein [Ｊ]. Hepatogastroenterology, 2003; 50(54)∶1825.
13.	Sato Y, Tsukada K, Hatakeyama K. Role of shear stress and immune responses in liver regeneration after a partial hepatectomy [Ｊ]. Surg Today, 1999; 29(1)∶1.
14.	Eguchi S, Yanaga K, Sugiyama N, et al. Relationship between portal venous flow and liver regeneration in patients after living donor rightlobe liver transplantation [Ｊ]. Liver Transpl, 2003; 9(6)∶547.
15.	Hasmall S, James N, Hedley K, et al. Mouse hepatocyte response to peroxisome proliferators: dependency on hepatic nonparenchymal cells and peroxisome proliferator activated receptor alpha (PPARalpha) [Ｊ]. Arch Toxicol, 2001; 75(6)∶357.
16.	Malik R, Selden C, Hodgson H. The role of nonparenchymal cells in liver growth [Ｊ]. Semin Cell Dev Biol, 2002; 13(6)∶425.
17.	Mochida S, Ishikawa K, Toshima K, et al. The mechanisms of hepatic sinusoidal endothelial cell regeneration: a possible communication system associated with vascular endothelial growth factor in liver cells [Ｊ]. J Gastroenterol Hepatol, 1998; 13(Suppl)∶S1.
18.	Sato T, ElAssal ON, Ono T, et al. Sinusoidal endothelial cell（下轉第468頁）（上承第458頁）proliferation and expression of angiopoietin/Tie family in regenerating rat liver [Ｊ]. J Hepatol, 2001; 34(5)∶690.
19.	Michalopoulos GK, DeFrances MC. Liver regeneration [Ｊ]. Science, 1997; 276(5309)∶60.
20.	Michalopoulos GK. Hepatocyte growth factor in liver growth and differentiation [Ｊ]. J Boil Chem, 1995; 98（11）∶219.
21.	Suzuki A, Iwama A, Miyashita H, et al. Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells [Ｊ]. Development, 2003; 130(11)∶2513.
22.	Daveau M, Scotte M, Francois A, et al. Hepatocyte growth factor, transforming growth factor alpha, and their receptors as combined markers of prognosis in hepatocellular carcinoma [Ｊ]. Mol Carcinog, 2003; 36(3)∶130.
23.	Koniaris LG, McKillop IH, Schwartz SI, et al. Liver regeneration [Ｊ]. J Am Coll Surg, 2003; 197(4)∶634.
24.	Debonera F, Aldeguer X, Shen X, et al. Activation of interleukin6/STAT3 and liver regeneration following transplantation [Ｊ]. J Surg Res, 2001; 96(2)∶289.
25.	Cressman DE, Greenbaum LE, DeAngelis RA, et al. Liver failure and defective hepatocyte regeneration in interleukin6deficient mice [Ｊ]. Science, 1996; 274(5291)∶1379.
26.	Kelm M, Feelisch M, Deussen A, et al. Release of endothelium derived nitric oxide in relation to pressure and flow [Ｊ]. Cardiovasc Res, 1991; 25(10)∶831.
27.	Hortelano S, Dewez B, Genaro AM, et al. Nitric oxide is released in regenerating liver after partial hepatectomy [Ｊ]. Hepatology, 1995; 21(3)∶776.
28.	李黔寧,應大君,戴光明,等. 一種簡易的平行板流動腔系統的應用 [Ｊ]. 醫用生物力學， 2002； 17（1）∶7.
29.	Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [Ｊ]. Science, 1985; 227(4693)∶1477.

1. Kahn D, van HoornHickman R, Terblanche J. Liver blood flow after partial hepatectomy in the pig [Ｊ]. J Surg Res, 1984; 37(4)∶290.
2. LeCouter J, Moritz DR, Li B, et al. Angiogenesisindependent endothelial protection of liver: role of VEGFR1 [Ｊ]. Science, 2003; 299(5608)∶890.
3. Xu B, Broome U, Uzunel M, et al. Capillarization of hepatic sinusoid by liver endothelial cellreactive autoantibodies in patients with cirrhosis and chronic hepatitis [Ｊ]. Am J Pathol, 2003; 163(4)∶1275.
4. Mendoza L, Carrascal T, De Luca M, et al. Hydrogen peroxide mediates vascular cell adhesion molecule1 expression from interleukin18activated hepatic sinusoidal endothelium: implications for circulating cancer cell arrest in the murine liver [Ｊ]. Hepatology, 2001; 34(2)∶298.
5. 朱瑾, 陳平,熊燕, 等. 大鼠肝竇內皮細胞分離、培養及其生物學特性的初步研究 [Ｊ]. 消化外科， 2004； 3（5）∶350.
6. Huet PM, Nagaoka MR, Desbiens G, et al. Sinusoidal endothelial cell and hepatocyte death following cold ischemiawarm reperfusion of the rat liver [Ｊ]. Hepatology, 2004; 39(4)∶1110.
7. Nedredal GI, Elvevold KH, Ytrebo LM, et al. Liver sinusoidal endothelial cells represents an important blood clearance system in pigs [Ｊ]. Comp Hepatol, 2003; 2(1)∶1.
8. Limmer A, Knolle PA. Liver sinusoidal endothelial cells: a new type of organresident antigenpresenting cell [Ｊ]. Arch Immunol Ther Exp (Warsz), 2001; 49(Suppl 1)∶S7.
9. Mendoza L, VidalVanaclocha F. Inflammatory response of tumoractivated hepatic sinusoidal endothelium as a target for the screening of metastasis chemopreventive drugs [Ｊ]. Methods Mol Med, 2003; 85∶107.
10. Thomson RY, Clarke AM. Role of portal blood supply in liver regeneration [Ｊ]. Nature, 1965; 208(8)∶392.
11. Schoen JM, Wang HH, Minuk GY, et al. Shear stressinduced nitric oxide release triggers the liver regeneration cascade [Ｊ]. Nitric Oxide, 2001; 5(5)∶453.
12. Kaneko T, Sugimoto H, Inoue S, et al. Doppler ultrasonography for postoperative hepatofugal portal blood flow of the intrahepatic portal vein [Ｊ]. Hepatogastroenterology, 2003; 50(54)∶1825.
13. Sato Y, Tsukada K, Hatakeyama K. Role of shear stress and immune responses in liver regeneration after a partial hepatectomy [Ｊ]. Surg Today, 1999; 29(1)∶1.
14. Eguchi S, Yanaga K, Sugiyama N, et al. Relationship between portal venous flow and liver regeneration in patients after living donor rightlobe liver transplantation [Ｊ]. Liver Transpl, 2003; 9(6)∶547.
15. Hasmall S, James N, Hedley K, et al. Mouse hepatocyte response to peroxisome proliferators: dependency on hepatic nonparenchymal cells and peroxisome proliferator activated receptor alpha (PPARalpha) [Ｊ]. Arch Toxicol, 2001; 75(6)∶357.
16. Malik R, Selden C, Hodgson H. The role of nonparenchymal cells in liver growth [Ｊ]. Semin Cell Dev Biol, 2002; 13(6)∶425.
17. Mochida S, Ishikawa K, Toshima K, et al. The mechanisms of hepatic sinusoidal endothelial cell regeneration: a possible communication system associated with vascular endothelial growth factor in liver cells [Ｊ]. J Gastroenterol Hepatol, 1998; 13(Suppl)∶S1.
18. Sato T, ElAssal ON, Ono T, et al. Sinusoidal endothelial cell（下轉第468頁）（上承第458頁）proliferation and expression of angiopoietin/Tie family in regenerating rat liver [Ｊ]. J Hepatol, 2001; 34(5)∶690.
19. Michalopoulos GK, DeFrances MC. Liver regeneration [Ｊ]. Science, 1997; 276(5309)∶60.
20. Michalopoulos GK. Hepatocyte growth factor in liver growth and differentiation [Ｊ]. J Boil Chem, 1995; 98（11）∶219.
21. Suzuki A, Iwama A, Miyashita H, et al. Role for growth factors and extracellular matrix in controlling differentiation of prospectively isolated hepatic stem cells [Ｊ]. Development, 2003; 130(11)∶2513.
22. Daveau M, Scotte M, Francois A, et al. Hepatocyte growth factor, transforming growth factor alpha, and their receptors as combined markers of prognosis in hepatocellular carcinoma [Ｊ]. Mol Carcinog, 2003; 36(3)∶130.
23. Koniaris LG, McKillop IH, Schwartz SI, et al. Liver regeneration [Ｊ]. J Am Coll Surg, 2003; 197(4)∶634.
24. Debonera F, Aldeguer X, Shen X, et al. Activation of interleukin6/STAT3 and liver regeneration following transplantation [Ｊ]. J Surg Res, 2001; 96(2)∶289.
25. Cressman DE, Greenbaum LE, DeAngelis RA, et al. Liver failure and defective hepatocyte regeneration in interleukin6deficient mice [Ｊ]. Science, 1996; 274(5291)∶1379.
26. Kelm M, Feelisch M, Deussen A, et al. Release of endothelium derived nitric oxide in relation to pressure and flow [Ｊ]. Cardiovasc Res, 1991; 25(10)∶831.
27. Hortelano S, Dewez B, Genaro AM, et al. Nitric oxide is released in regenerating liver after partial hepatectomy [Ｊ]. Hepatology, 1995; 21(3)∶776.
28. 李黔寧,應大君,戴光明,等. 一種簡易的平行板流動腔系統的應用 [Ｊ]. 醫用生物力學， 2002； 17（1）∶7.
29. Frangos JA, Eskin SG, McIntire LV, et al. Flow effects on prostacyclin production by cultured human endothelial cells [Ｊ]. Science, 1985; 227(4693)∶1477.

《中國普外基礎與臨床雜志》

切應力對大鼠肝竇內皮細胞分泌功能的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

切應力對大鼠肝竇內皮細胞分泌功能的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料