選擇性COX-2抑制劑尼美舒利對大鼠乳腺癌的影響及機理研究_《中國普外基礎與臨床雜志》

作者：

郭貴龍 ¹ , 張筱驊 ¹ , 姚榛祥 ²

1. 浙江省溫州醫學院附屬第一醫院腫瘤外科（溫州325000）;;
2.重慶醫科大學附屬第一醫院普外科（重慶400016）;

關鍵詞：

尼美舒利二甲基苯并蒽乳腺腫瘤凋亡

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【摘要】目的研究選擇性環氧合酶-2（COX-2）抑制劑尼美舒利（nimesulide，NIM）對二甲基苯并蒽（DMBA）誘導的Wistar大鼠乳腺癌產生的影響，探討其抗腫瘤機理。
方法將大鼠按隨機數字表法分為致癌組、NIM組、飲食對照組及藥物對照組，觀察乳腺腫瘤發生的潛伏期、誘發率以及腫瘤的數目和體積。用TUNEL法和PCNA免疫組化法分別檢測細胞凋亡和增殖指數。
結果 NIM組大鼠乳腺腫瘤潛伏期比致癌組明顯延長〔（115±14.8） d vs (84±15.6） d，P＜0.01〕，腫瘤誘發率明顯低于致癌組（46.4% vs 69.2%, P＜0.05），腫瘤數目及體積明顯少于致癌組〔（0.82±0.31）個 vs （1.52±0.43）個，（3.24±1.21） cm3 vs （6.03±1.84） cm3，P＜0.05〕； NIM組腫瘤細胞的增殖指數下降，凋亡指數明顯增高，與致癌組相比差異有顯著性意義〔(20.25±4.97)% vs (36.36±5.02）%, (42.54±13.24)% vs (18.14±6.66)%,P＜0.05〕。
結論 NIM通過抑制腫瘤細胞的增殖、誘導凋亡，降低了DMBA誘發的大鼠乳腺癌的發生率。

引用本文： 郭貴龍,張筱驊,姚榛祥. 選擇性COX-2抑制劑尼美舒利對大鼠乳腺癌的影響及機理研究. 中國普外基礎與臨床雜志, 2005, 12(3): 219-222. doi: 復制

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9.	Harris RE, Chlebowski RT, Jackson RD, et al. Breast cancer and nonsteroidalantiinflammatory drugs: prospective results from the women’s health initiative [Ｊ]. Cancer Res, 2003; 63(15)∶6096.
10.	Egan KM, Stampfer MJ, Geovannrcci E, et al. Prospective study of regular aspirin use and the risk of breast cancer [Ｊ]. J Natl Cancer Inst, 1996; 88(14)∶988.
11.	Denda A, Kitayama W, Murata A, et al. Increased expression of cyclooxygenase2 protein during rat hepatocarcinogenesis caused by a cholinedeficient, Lamino aciddefined diet and chemopreventive efficacy of a specific inhibitor, nimesulide [Ｊ]. Carcinogenesis, 2002; 23(2)∶245.
12.	Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase2 [Ｊ]. Cell, 1995; 83(4)∶493.
13.	Tardieu D, Jaeg JP, Deloly A, et al. The COX2 inhibitor nimesulide suppresses superoxide and 8hydroxydeoxyguanosine formation, and stimulates apoptosis in mucosa during early colonic inflammation in rats [Ｊ]. Carcinogenesis, 2000; 21(5)：973.

1. Ristimaki A, Sivula A, Lundin J, et al. Prognostic significance of elevated cyclooxygenase2 expression in breast cancer [Ｊ]. Cancer Res， 2002; 62(3)∶ 632.
2. 李建英，于皆平，羅和生，等. 選擇性COX2抑制劑對胃癌細胞增殖的影響 [Ｊ]. 癌癥, 2002; 21(6)∶625.
3. Tian G, Yu JP, Luo HS, et al. Effect of nimesulide on proliferation and apoptosis of human hepatoma SMMC7721 cells [Ｊ]. World J Gastroenterol, 2002; 8(3)∶483.
4. Nakatsugi S, Ohta T, Kawamori T, et al. Chemprevention by nimesulide, a selective cyclooxygenase2 inhibitor, of 2amin1methyl6phenylinidazo pyr I dineinduced mammary gland carcinogenesis in rats [Ｊ]. Jpn J Cancer Res, 2000; 91（9）∶886.
5. 姜軍，陳意生，柳鳳軒，等. 二甲基苯蒽誘導大鼠乳腺發生的組織形態學研究 [Ｊ]. 中國腫瘤臨床,1998; 25（6）∶436.
6. Rasso J, Gusterson BA, Rogers AE, et al. Comparative study of human and rat mammary tumorigenesis [Ｊ]. Lab Invest, 1990; 62(3)∶244.
7. 鄧敏，蘇寧，朝月明,等. 三苯氧胺對二甲基苯蒽誘發的大鼠乳腺癌的影響 [Ｊ]. 蘇州醫學院學報, 1999; 19(9)∶961.
8. Russo J, Russo IH. Experimentally induced mammary tumors in rats [Ｊ]. Breast Cancer Res Treat, 1996; 39(1)∶7.
9. Harris RE, Chlebowski RT, Jackson RD, et al. Breast cancer and nonsteroidalantiinflammatory drugs: prospective results from the women’s health initiative [Ｊ]. Cancer Res, 2003; 63(15)∶6096.
10. Egan KM, Stampfer MJ, Geovannrcci E, et al. Prospective study of regular aspirin use and the risk of breast cancer [Ｊ]. J Natl Cancer Inst, 1996; 88(14)∶988.
11. Denda A, Kitayama W, Murata A, et al. Increased expression of cyclooxygenase2 protein during rat hepatocarcinogenesis caused by a cholinedeficient, Lamino aciddefined diet and chemopreventive efficacy of a specific inhibitor, nimesulide [Ｊ]. Carcinogenesis, 2002; 23(2)∶245.
12. Tsujii M, DuBois RN. Alterations in cellular adhesion and apoptosis in epithelial cells overexpressing prostaglandin endoperoxide synthase2 [Ｊ]. Cell, 1995; 83(4)∶493.
13. Tardieu D, Jaeg JP, Deloly A, et al. The COX2 inhibitor nimesulide suppresses superoxide and 8hydroxydeoxyguanosine formation, and stimulates apoptosis in mucosa during early colonic inflammation in rats [Ｊ]. Carcinogenesis, 2000; 21(5)：973.

《中國普外基礎與臨床雜志》

選擇性COX-2抑制劑尼美舒利對大鼠乳腺癌的影響及機理研究

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《中國普外基礎與臨床雜志》

選擇性COX-2抑制劑尼美舒利對大鼠乳腺癌的影響及機理研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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