誘導HO-1改善氧化應激狀態減輕老年供肝缺血-再灌注損傷△_《中國普外基礎與臨床雜志》

作者：

王科 , 王學浩 , 張峰 , 李相成 , 錢曉峰范燁

江蘇省肝臟移植中心，南京醫科大學第一附屬醫院肝臟外科（南京210029）;

關鍵詞：

老年供體血紅素氧合酶-1 缺血-再灌注損傷

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【摘要】目的觀察誘導血紅素氧合酶-1（HO-1）生成能否減輕老年供肝移植后缺血再灌注損傷。
方法取老年（16個月齡）SD大鼠5只和成年（3個月齡）大鼠5只，分別測定其肝臟組織中超氧化物歧化酶(SOD)和過氧化氫酶(CAT)的活性及維生素E(Vit E)、維生素C(Vit C)和丙二醛(MDA)含量；另取老年SD大鼠60只作為供體，隨機均分為血晶素（Hemin）組和對照組，分別于取肝前24 h腹腔注射Hemin或生理鹽水，然后將其肝臟移植給同種大鼠，同時觀察再灌注后肝臟組織學變化和細胞凋亡。
結果老年大鼠肝臟組織中SOD活性明顯低于成年大鼠，Vit C含量亦少（P＜0.05），而MDA明顯增高（P＜0.05）。用Hemin預處理的老年SD大鼠供肝在切取前，SOD活性增加，Vit E、Vit C含量增加，MDA含量降低（P＜0.05）；肝移植后血清ALT明顯降低，肝組織HO-1活性明顯增高，肝臟組織學檢查示再灌注后凋亡細胞明顯減少（P＜0.05）。
結論老年SD大鼠肝臟存在氧化應激和脂質過氧化狀態，HO-1可通過改善這種狀態而減輕其缺血-再灌注損傷。

引用本文： 王科,王學浩,張峰,李相成,錢曉峰范燁. 誘導HO-1改善氧化應激狀態減輕老年供肝缺血-再灌注損傷△. 中國普外基礎與臨床雜志, 2005, 12(2): 109-112. doi: 復制

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1. Kohli V, Selzner M, Madden JF, et al. Endothelial cell and hepatocyte deaths occur by apoptosis after ischemiareperfusion injury in the rat liver [Ｊ]. Transplantation, 1999; 67(8)1099.
2. Deschenes M, Forbes C, Tchervenkov J, et al. Use of older donor livers is associated with more extensive ischemic damage on intraoperative biopsies during liver transplantation [Ｊ]. Liver Transpl Surg, 1999; 5(5)357.
3. Busuttil RW, Tanaka K. The utility of marginal donors in liver transplantation [Ｊ]. Liver Transpl, 2003; 9(7)651.
4. Yersiz H, Shaked A, Olthoff K, et al. Correlation between donor age and the pattern of liver graft recovery after transplantation [Ｊ]. Transplantation, 1995; 60(8)790.
5. Busquets J, Xiol X, Figueras J, et al. The impact of donor age on liver transplantation: influence of donor age on early liver function and on subsequent patient and graft survival [Ｊ]. Transplantation, 2001; 71(12)1765.
6. Briceno J, Marchal T, Padillo J, et al. Influence of marginal donors on liver preservation injury [Ｊ]. Transplantation, 2002; 74(4)522.
7. Sass G, Soares MC, Yamashita K, et al. Heme oxygenase1 and its reaction product, carbon monoxide, prevent inflammationrelated apoptotic liver damage in mice [Ｊ]. Hepatology, 2003; 38(4)909.
8. Kato Y, Shimazu M, Kondo M, et al. Bilirubin rinse: A simple protectant against the rat liver graft injury mimicking heme oxygenase1 preconditioning [Ｊ]. Hepatology, 2003; 38(2)364.
9. Schwartzman RA, Cidlowski JA. Apoptosis: the biochemistry and molecular biology of programmed cell death [Ｊ]. Endocr Rev, 1993; 14(2)133.

《中國普外基礎與臨床雜志》

誘導HO-1改善氧化應激狀態減輕老年供肝缺血-再灌注損傷△

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《中國普外基礎與臨床雜志》

誘導HO-1改善氧化應激狀態減輕老年供肝缺血-再灌注損傷△

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