N-硝基精氨酸甲酯對大腸癌裸鼠移植瘤生長的抑制作用△_《中國普外基礎與臨床雜志》

作者：

于麗波 , 孫文洲 , 董新舒

哈爾濱醫科大學附屬腫瘤醫院普外科(哈爾濱150040);

關鍵詞：

大腸癌N-硝基精氨酸甲酯腫瘤血管形成

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目的　觀察一氧化氮合酶（NOS）抑制劑N-硝基精氨酸甲酯(L-NAME)抑制大腸癌生長的體內效應，及其對大腸癌移植瘤相關血管形成的影響。方法　將20只BALB/c（nu/nu）裸鼠按隨機數字表法均分為對照組和實驗組,大腸癌細胞系SL174T種植入裸鼠皮下，形成移植瘤。對照組0.2 ml生理鹽水灌胃,實驗組經口灌入L-NAME,4 mg/次，3次/周，共4周。然后記錄腫瘤大小，采用免疫組化法檢測微血管密度。結果　L-NAME對裸鼠大腸癌移植瘤的腫瘤重量抑瘤率為41.36%；腫瘤體積抑瘤率為43.48%。實驗組大腸癌組織微血管密度（14.83±2.10）個，明顯低于對照組的（21.04±3.11）個，兩組間差異有統計學意義（P＜0.05）。結論　L-NAME具有抑制裸鼠大腸癌腫瘤生長和對抗腫瘤新生血管生成的作用。

引用本文： 于麗波,孫文洲,董新舒. N-硝基精氨酸甲酯對大腸癌裸鼠移植瘤生長的抑制作用△. 中國普外基礎與臨床雜志, 2006, 13(5): 542-544. doi: 復制

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1. 　Fukumura D, Jain RK. Role of nitric oxide in angiogenesis and microcirculation in tumors [Ｊ]. Cancer Metastasis Rev, 1998; 17(1)∶ 77.
2. 　Jenkins DC, Charles IG, Thomsen LL, et al. Roles of nitric oxide in tumor growth [Ｊ]. Proc Natl Acad Sci USA, 1995; 92(10)∶ 4392.
3. 　Kojima M, Morisaki T, Tsukahara Y, et al. Nitric oxide synthase expression and nitric oxide production in human colon carcinoma tissue [Ｊ]. J Surg Oncol, 1999; 70(4)∶ 222.
4. 　李學農，周　軍，丁彥青. 一氧化氮合酶在人大腸癌細胞中的表迭及意義 [Ｊ]. 中國腫瘤臨床， 2001； 28（2）∶ 94.
5. 　Lagares-Garcia JA, Moore RA, Collier B, et al. Nitric oxide synthase as a marker in colorectal carcinoma [Ｊ].Am Surg, 2001; 67(7)∶ 709.
6. 　Ahn B, Ohshima H. Suppression of intestinal polyposis in Apc(Min/+) mice by inhibiting nitric oxide production [Ｊ]. Cancer Res, 2001; 61(23)∶ 8357.
7. 　Watanabe K, Kawamori T, Nakatsugi S, et al. COX-2 and iNOS, good targets for chemoprevention of colon cancer [Ｊ]. Biofactors, 2000; 12(1-4)∶ 129.
8. 　Orucevic A, Lala PK. Effects of N(G)-Nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, on IL-2-induced LAK cell generation in vivo and in vitro in healthy and tumor-bearing mice [Ｊ]. Cell Immunol, 1996; 169(1)∶ 125.
9. 　Iwasaki T, Higashiyama M, Kuriyama K, et al. NG-nitro-L-arginine methyl ester inhibits bone metastasis after modified intracardiac injection of human breast cancer cells in a nude mouse model [Ｊ]. Jpn J Cancer Res, 1997; 88(9)∶ 861.

《中國普外基礎與臨床雜志》

N-硝基精氨酸甲酯對大腸癌裸鼠移植瘤生長的抑制作用△

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《中國普外基礎與臨床雜志》

N-硝基精氨酸甲酯對大腸癌裸鼠移植瘤生長的抑制作用△

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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