中國人與西方人胰腺癌組織中Ki-ras基因第12密碼子突變表型的比較△_《中國普外基礎與臨床雜志》

作者：

董明 , 周建平 , 張浩 , 郭克建 , 田雨霖 , 董雨亭

中國醫科大學附屬第一醫院普外科（沈陽 110001）;

關鍵詞：

胰腺癌Ki-ras基因流行病學

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　探討中國胰腺癌患者Ki-ras基因第12密碼子突變表型的特點及與西方國家胰腺癌的區別。方法　采用PCR擴增靶基因片段，Dot blot雜交的方法檢測59例中國胰腺癌患者標本中Ki-ras基因第12密碼子突變表型。利用Medline檢索相關西方國家文獻報道的胰腺癌患者Ki-ras基因第12密碼子突變表型，并與本組資料進行對比。結果　本組患者Ki-ras基因第12密碼子突變頻繁（76.3%）。與西方國家文獻報道的資料比較顯示，各國胰腺癌患者中Ki-ras基因第12密碼子突變頻率差異無統計學意義（P＞0.05），但突變的表型及第二堿基突變的轉換和顛換比的差異存在統計學意義（P＜0.05，P＜0.01，P＜0.001）。結論　Ki-ras基因第12密碼子突變在中國胰腺癌患者較頻繁，其突變表型在各國胰腺癌患者中可能存在差異。

引用本文： 董明,周建平,張浩,郭克建,田雨霖,董雨亭. 中國人與西方人胰腺癌組織中Ki-ras基因第12密碼子突變表型的比較△. 中國普外基礎與臨床雜志, 2006, 13(5): 524-528. doi: 復制

1.	Hruban RH, van Mansfeld AD, Offerhaus GJ, et al. K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization [J]. Am J Pathol, 1993; 143(2)∶545.
2.	Pellegata NS, Sessa F, Renault B, et al. K-ras and p53 gene mutations in pancreatic cancer: ductal and nonductal tumors progress through different genetic lesions [J]. Cancer Res, 1994; 54(6)∶1556.
3.	Sugio K, Gazdar AF, Albores-Saavedra J, et al. High yields of K-ras mutations in intraductal papillary mucinous tumors and invasive adenocarcinomas induced by N-nitroso(2-hydroxypropyl)(2-oxopropyl)amine in the pancreas of female Syrian hamsters [J]. Carcinogenesis, 1996; 17(2)∶303.
4.	Scarpa A, Capelli P, Villaneuva A, et al. Pancreatic cancer in Europe: Ki-ras gene mutation pattern shows geographical differences [J]. Int J Cancer, 1994; 57(2)∶167.
5.	Tada M, Ohashi M, Shiratori Y, et al. Analysis of K-ras gene mutation in hyperplastic duct cells of the pancreas without pancreatic disease [J]. Gastroenterology, 1996; 110(1)∶227.
6.	van Es JM, Polak MM, van den Berg FM, et al. Molecular markers for diagnostic cytology of neoplasms in the head region of the pancreas: mutation of K-ras and overexpression of the p53 protein product [J]. J Clin Pathol, 1995; 48(3)∶218.
7.	Grunewald K, Lyons J, Frohlich A. High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas [J]. Int J Cancer, 1989; 43(6)∶1037.
8.	Sobin LH, Wittekind CH Eds. TNM classification of malignant tu-mours [M]. New York: John Wiley and Sons, Inc, 1997∶87-90.
9.	Neuman WL, Wasylyshyn ML, Jacoby R, et al. Evidence for a common molecular pathogenesis in colorectal, gastric, and pancreatic cancer [J]. Genes Chromosomes Cancer, 1991; 3(6)∶468.
10.	Burmer GC, Rabinovitch PS, Loeb LA. Frequency and spectrum of c-Ki-ras mutations in human sporadic colon carcinoma, carcinomas arising in ulcerative colitis, and pancreatic adenocarcinoma [J]. Environ Health Perspect, 1991; 93∶27.
11.	Lemoine NR, Jain S, Hughes CM, et al. Ki-ras oncogene activation in preinvasive pancreatic cancer [J]. Gastroenterology, 1992; 102(1)∶230.
12.	Mulcahy HE, Lyautey J, Lederrey C, et al. A prospective study of K-ras mutations in the plasma of pancreatic cancer patients [J]. Clin Cancer Res, 1998; 4(2)∶271.
13.	Berrozpe G, Schaeffer J, Peinado MA, et al. Comparative analysis of mutations in the p53 and K-ras genes in pancreatic cancer [J]. nt J Cancer, 1994; 58(2)∶185.
14.	Smit VT, Boot AJ, Smits AM, et al. KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas [J]. ucleic Acids Res, 1988; 16(16)∶7773.
15.	Goelz SE, Hamilton SR, Vogelstein B. et al. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue [J]. Biochem Biophys Res Commun, 1985; 130(1)∶118.
16.	Tominaga S. Epidemiology of pancreatic cancer [J]. Gan To Kagaku Ryoho, 1992; 19(14)∶2297.
17.	Nagata Y, Abe M, Motoshima K, et al. Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japanese [J]. Jpn J Cancer Res, 1990; 81(2)∶135.
18.	Castells A, Puig P, Mora J, et al. K-ras mutations in DNA extracted from the plasma of patients with pancreatic carcinoma: diagnostic utility and prognostic significance [J]. J Clin Oncol, 1999; 17(2)∶578.
19.	Song MM, Nio Y, Dong M, et al. Comparison of K-ras point mutations at codon 12 and p21 expression in pancreatic cancer between Japanese and Chinese patients [J]. J Surg Oncol, 2000; 75(3)∶176.
20.	Parada LF, Land H, Weinberg RA, et al. Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation [J]. Nature, 1984; 312(5995)∶649.
21.	Zambetti GP, Olson D, Labow M, et al. A mutant p53 protein is required for maintenance of the transformed phenotype in cells transformed with p53 plus ras cDNAs [J]. Proc Natl Acad Sci USA， 1992; 89(9)∶3952.

1. 　Hruban RH, van Mansfeld AD, Offerhaus GJ, et al. K-ras oncogene activation in adenocarcinoma of the human pancreas. A study of 82 carcinomas using a combination of mutant-enriched polymerase chain reaction analysis and allele-specific oligonucleotide hybridization [J]. Am J Pathol, 1993; 143(2)∶545.
2. 　Pellegata NS, Sessa F, Renault B, et al. K-ras and p53 gene mutations in pancreatic cancer: ductal and nonductal tumors progress through different genetic lesions [J]. Cancer Res, 1994; 54(6)∶1556.
3. 　Sugio K, Gazdar AF, Albores-Saavedra J, et al. High yields of K-ras mutations in intraductal papillary mucinous tumors and invasive adenocarcinomas induced by N-nitroso(2-hydroxypropyl)(2-oxopropyl)amine in the pancreas of female Syrian hamsters [J]. Carcinogenesis, 1996; 17(2)∶303.
4. 　Scarpa A, Capelli P, Villaneuva A, et al. Pancreatic cancer in Europe: Ki-ras gene mutation pattern shows geographical differences [J]. Int J Cancer, 1994; 57(2)∶167.
5. 　Tada M, Ohashi M, Shiratori Y, et al. Analysis of K-ras gene mutation in hyperplastic duct cells of the pancreas without pancreatic disease [J]. Gastroenterology, 1996; 110(1)∶227.
6. 　 van Es JM, Polak MM, van den Berg FM, et al. Molecular markers for diagnostic cytology of neoplasms in the head region of the pancreas: mutation of K-ras and overexpression of the p53 protein product [J]. J Clin Pathol, 1995; 48(3)∶218.
7. 　Grunewald K, Lyons J, Frohlich A. High frequency of Ki-ras codon 12 mutations in pancreatic adenocarcinomas [J]. Int J Cancer, 1989; 43(6)∶1037.
8. 　Sobin LH, Wittekind CH Eds. TNM classification of malignant tu-mours [M]. New York: John Wiley and Sons, Inc, 1997∶87-90.
9. 　Neuman WL, Wasylyshyn ML, Jacoby R, et al. Evidence for a common molecular pathogenesis in colorectal, gastric, and pancreatic cancer [J]. Genes Chromosomes Cancer, 1991; 3(6)∶468.
10. 　Burmer GC, Rabinovitch PS, Loeb LA. Frequency and spectrum of c-Ki-ras mutations in human sporadic colon carcinoma, carcinomas arising in ulcerative colitis, and pancreatic adenocarcinoma [J]. Environ Health Perspect, 1991; 93∶27.
11. 　Lemoine NR, Jain S, Hughes CM, et al. Ki-ras oncogene activation in preinvasive pancreatic cancer [J]. Gastroenterology, 1992; 102(1)∶230.
12. 　Mulcahy HE, Lyautey J, Lederrey C, et al. A prospective study of K-ras mutations in the plasma of pancreatic cancer patients [J]. Clin Cancer Res, 1998; 4(2)∶271.
13. 　Berrozpe G, Schaeffer J, Peinado MA, et al. Comparative analysis of mutations in the p53 and K-ras genes in pancreatic cancer [J]. nt J Cancer, 1994; 58(2)∶185.
14. 　Smit VT, Boot AJ, Smits AM, et al. KRAS codon 12 mutations occur very frequently in pancreatic adenocarcinomas [J]. ucleic Acids Res, 1988; 16(16)∶7773.
15. 　Goelz SE, Hamilton SR, Vogelstein B. et al. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue [J]. Biochem Biophys Res Commun, 1985; 130(1)∶118.
16. 　Tominaga S. Epidemiology of pancreatic cancer [J]. Gan To Kagaku Ryoho, 1992; 19(14)∶2297.
17. 　Nagata Y, Abe M, Motoshima K, et al. Frequent glycine-to-aspartic acid mutations at codon 12 of c-Ki-ras gene in human pancreatic cancer in Japanese [J]. Jpn J Cancer Res, 1990; 81(2)∶135.
18. 　Castells A, Puig P, Mora J, et al. K-ras mutations in DNA extracted from the plasma of patients with pancreatic carcinoma: diagnostic utility and prognostic significance [J]. J Clin Oncol, 1999; 17(2)∶578.
19. 　Song MM, Nio Y, Dong M, et al. Comparison of K-ras point mutations at codon 12 and p21 expression in pancreatic cancer between Japanese and Chinese patients [J]. J Surg Oncol, 2000; 75(3)∶176.
20. 　Parada LF, Land H, Weinberg RA, et al. Cooperation between gene encoding p53 tumour antigen and ras in cellular transformation [J]. Nature, 1984; 312(5995)∶649.
21. 　Zambetti GP, Olson D, Labow M, et al. A mutant p53 protein is required for maintenance of the transformed phenotype in cells transformed with p53 plus ras cDNAs [J]. Proc Natl Acad Sci USA， 1992; 89(9)∶3952.

《中國普外基礎與臨床雜志》

中國人與西方人胰腺癌組織中Ki-ras基因第12密碼子突變表型的比較△

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

中國人與西方人胰腺癌組織中Ki-ras基因第12密碼子突變表型的比較△

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料