肺耐藥蛋白在胰腺癌細胞中的表達及意義_《中國普外基礎與臨床雜志》

作者：

郭俊超 , 閆長青 , 廖泉 , 趙玉沛

【作者單位】中國醫學科學院北京協和醫院基本外科(北京100730);;
通訊作者：趙玉沛，E-mail: zhao8028@263.net;

關鍵詞：

胰腺腫瘤多藥耐藥獲得性耐藥

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【摘要】　目的　探討肺耐藥蛋白(lung resistance protein, LRP)在胰腺癌細胞株中的表達及意義。方法　采用反轉錄多聚酶鏈反應(RT-PCR)和免疫細胞化學方法檢測8株胰腺癌細胞株（SW1990、PCT-2、PCT-3、PCT-4、Aspc-1、Capan-1、Mia-PaCa-2及Panc-1）中LRP在基因水平和蛋白水平的表達。結果　 RT-PCR結果顯示，在胰腺癌細胞株PCT-2中未檢測到LRP mRNA的表達，在PCT-4、Aspc-1和Panc-1中，LRP mRNA表達條帶較強；在SW1990、PCT-3、Capan-1和Mia-PaCa-2中LRP mRNA表達條帶較弱；半定量平均表達水平為0.56±0.33。免疫細胞化學結果證實,在PCT-2細胞中無LRP蛋白表達，PCT-3細胞中LRP蛋白弱表達，SW1990、Aspc-1和Capan-1細胞中LRP蛋白中度表達，而在PCT-4、Mia-PaCa-2和Panc-1細胞中可見LRP蛋白的過度表達。結論　在胰腺癌細胞中存在LRP的先天性表達，LRP過表達可能是介導胰腺癌細胞先天性耐藥的重要機理之一。

引用本文： 郭俊超,閆長青,廖泉,趙玉沛. 肺耐藥蛋白在胰腺癌細胞中的表達及意義. 中國普外基礎與臨床雜志, 2006, 13(5): 502-505. doi: 復制

1.	郭俊超. 多藥耐藥基因與胰腺癌化療 [J]. 胰腺病學, 2002; 2(4)∶247.
2.	張立陽, 趙玉沛, 廖　泉, 等. MDR1在胰腺癌細胞株中的表達及其意義 [J]. 中華實驗外科雜志， 2003; 20(5)∶475.
3.	張立陽, 趙玉沛, 吳元德，等. 胰腺癌阿霉素耐藥細胞株SW1990/ADM的建立及其耐藥機理研究 [J]. 中國普外基礎與臨床雜志， 2005; 12(1)∶46.
4.	潘　博, 郭俊超, 廖　泉, 等. 吉西他濱與胰腺癌化療耐藥 [J]. 中國普外基礎與臨床雜志, 2005; 12(5)∶503.
5.	郭俊超, 趙玉沛, 廖　泉, 等. 胰腺癌耐藥細胞株SW1990/FU的建立、鑒定及生物學特性 [J]. 中國醫學科學院學報, 2005; 27(5)∶592.
6.	Berger W, Elbling L, Micksche M. Expression of the major va-ult protein LRP in human non-small-cell lung cancer cells: activation by short-term exposure to antineoplastic drugs [J].Int J Cancer, 2000; 88(2)∶293.
7.	Fan K, Fan D, Cheng L, et al. Expression of multidrug resistance-related markers in gastric cancer [J]. Anticancer Res, 2000; 20(6C)∶4809.
8.	Raidl M, Berger W, Schulte-Hermann R, et al. Expression of the lung resistance-related protein in human and rat hepatocarcinogenesis [J]. Am J Physiol Gastrointest Liver Physiol, 2002; 283(5)∶G1117.
9.	Schneider J, Gonzalez-Roces S, Pollan M, et al. Expression of LRP and MDR1 in locally advanced breast cancer predicts axillary node invasion at the time of rescue mastectomy after induction chemotherapy [J]. Breast Cancer Res, 2001; 3(3)∶183.
10.	Scheper RJ, Broxterman HJ, Scheffer GL, et al. Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance [J]. Cancer Res, 1993; 53(7)∶1475.
11.	Izquierdo MA, Scheffer GL, Flens MJ, et al. Major vault protein LRP-related multidrug resistance [J]. Eur J Cancer, 1996; 32A(6)∶979.
12.	Mandoky L, Geczi L, Doleschall Z, et al. Expression and prognostic value of the lung resistance-related protein(LRP) in germ cell testicular tumors [J]. Anticancer Res, 2004; 24(2C)∶1097.
13.	Olson DP, Taylor BJ, La M, et al. The prognostic significance of P-glycoprotein, multidrug resistance-related protein 1 and lung resistance protein in pediatric acute lymphoblastic leukemia: a retrospective study of 295 newly diagnosed patients by the Children’s Oncology Group [J]. Leuk Lymphoma, 2005; 46(5)∶681.
14.	Izquierdo MA, Scheffer GL, Flens MJ, et al. Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors [J]. Am J Pathol, 1996; 148(3)∶877.
15.	Liu B, Staren ED, Iwamura T, et al. Mechanisms of taxotere-related drug resistance in pancreatic carcinoma [J]. J Surg Res, 2001; 99(2)∶179.
16.	Qadir M, O’Loughlin KL, Fricke SM, et al. Cyclosporin A is a broad-spectrum multidrug resistance modulator [J]. Clin Cancer Res, 2005; 11(6)∶2320.

1. 　郭俊超. 多藥耐藥基因與胰腺癌化療 [J]. 胰腺病學, 2002; 2(4)∶247.
2. 　張立陽, 趙玉沛, 廖　泉, 等. MDR1在胰腺癌細胞株中的表達及其意義 [J]. 中華實驗外科雜志， 2003; 20(5)∶475.
3. 　張立陽, 趙玉沛, 吳元德，等. 胰腺癌阿霉素耐藥細胞株SW1990/ADM的建立及其耐藥機理研究 [J]. 中國普外基礎與臨床雜志， 2005; 12(1)∶46.
4. 　潘　博, 郭俊超, 廖　泉, 等. 吉西他濱與胰腺癌化療耐藥 [J]. 中國普外基礎與臨床雜志, 2005; 12(5)∶503.
5. 　郭俊超, 趙玉沛, 廖　泉, 等. 胰腺癌耐藥細胞株SW1990/FU的建立、鑒定及生物學特性 [J]. 中國醫學科學院學報, 2005; 27(5)∶592.
6. 　Berger W, Elbling L, Micksche M. Expression of the major va-ult protein LRP in human non-small-cell lung cancer cells: activation by short-term exposure to antineoplastic drugs [J].Int J Cancer, 2000; 88(2)∶293.
7. 　Fan K, Fan D, Cheng L, et al. Expression of multidrug resistance-related markers in gastric cancer [J]. Anticancer Res, 2000; 20(6C)∶4809.
8. 　Raidl M, Berger W, Schulte-Hermann R, et al. Expression of the lung resistance-related protein in human and rat hepatocarcinogenesis [J]. Am J Physiol Gastrointest Liver Physiol, 2002; 283(5)∶G1117.
9. 　Schneider J, Gonzalez-Roces S, Pollan M, et al. Expression of LRP and MDR1 in locally advanced breast cancer predicts axillary node invasion at the time of rescue mastectomy after induction chemotherapy [J]. Breast Cancer Res, 2001; 3(3)∶183.
10. 　Scheper RJ, Broxterman HJ, Scheffer GL, et al. Overexpression of a M(r) 110,000 vesicular protein in non-P-glycoprotein-mediated multidrug resistance [J]. Cancer Res, 1993; 53(7)∶1475.
11. 　Izquierdo MA, Scheffer GL, Flens MJ, et al. Major vault protein LRP-related multidrug resistance [J]. Eur J Cancer, 1996; 32A(6)∶979.
12. 　Mandoky L, Geczi L, Doleschall Z, et al. Expression and prognostic value of the lung resistance-related protein(LRP) in germ cell testicular tumors [J]. Anticancer Res, 2004; 24(2C)∶1097.
13. 　Olson DP, Taylor BJ, La M, et al. The prognostic significance of P-glycoprotein, multidrug resistance-related protein 1 and lung resistance protein in pediatric acute lymphoblastic leukemia: a retrospective study of 295 newly diagnosed patients by the Children’s Oncology Group [J]. Leuk Lymphoma, 2005; 46(5)∶681.
14. 　Izquierdo MA, Scheffer GL, Flens MJ, et al. Broad distribution of the multidrug resistance-related vault lung resistance protein in normal human tissues and tumors [J]. Am J Pathol, 1996; 148(3)∶877.
15. 　Liu B, Staren ED, Iwamura T, et al. Mechanisms of taxotere-related drug resistance in pancreatic carcinoma [J]. J Surg Res, 2001; 99(2)∶179.
16. 　Qadir M, O’Loughlin KL, Fricke SM, et al. Cyclosporin A is a broad-spectrum multidrug resistance modulator [J]. Clin Cancer Res, 2005; 11(6)∶2320.

《中國普外基礎與臨床雜志》

肺耐藥蛋白在胰腺癌細胞中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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《中國普外基礎與臨床雜志》

肺耐藥蛋白在胰腺癌細胞中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料