內源性光動力療法對人結腸癌SW480細胞cAMP和cGMP的影響_《中國普外基礎與臨床雜志》

作者：

鄭江華 ¹ , 時德 ¹ , 陳祖林 ² , 肖衛東 ² , 梁平 ²

1.重慶醫科大學附屬第一醫院血管外科、重慶市普外科學重點實驗室（重慶400016）;;
2.第三軍醫大學附屬新橋醫院普外科（重慶400037）;;
通訊作者：陳祖林，Email： chenzulin@163.com;

關鍵詞：

內源性光動力療法 SW480細胞環磷酸腺苷環磷酸鳥苷

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的觀察內源性光動力療法(photodynamic therapy, PDT)對人結腸癌SW480細胞環磷酸腺苷（cAMP）和環磷酸鳥苷（cGMP）的影響。
方法將SW480細胞分為4組：對照組、光照組、 δ氨基酮戊酸 (ALA)組、內源性PDT(ALAPDT)組，用放射免疫法檢測光照后30、60、90及120 min各組細胞的cAMP和cGMP的濃度。
結果cAMP濃度在監測時間內，ALAPDT組在30 min時最高，明顯高于其余各組相應時相及同一組內其余時相（P均＜0.001）；在60、90及120 min時與其它各組相應時相比較，差異均無統計學意義；其余各組在監測時間范圍內，組間及組內不同時相間差異均無統計學意義。cGMP濃度在各組細胞的組間及同組內不同時相間差異均無統計學意義。
結論內源性PDT在殺傷腫瘤細胞的同時，SW480細胞也能通過細胞內cAMP濃度的瞬時升高產生保護作用，抑制細胞凋亡。

引用本文： 鄭江華,時德,陳祖林,肖衛東,梁平. 內源性光動力療法對人結腸癌SW480細胞cAMP和cGMP的影響. 中國普外基礎與臨床雜志, 2006, 13(3): 271-274. doi: 復制

1.	Eur J Cancer， 1996; 32A Suppl 5∶S23.
2.	Van Cutsem E. A glimpse of the future. New directions in the treatment of colorectal cancer [Ｊ].
3.	Almeida RD, Manadas BJ, Carvalho AP, et al. Intracellular signaling mechanisms in photodynamic therapy [Ｊ]. Biochim Biophys Acta， 2004; 1704(2)∶59.
4.	Muhl H, Nitsch D, Sandau K, et al. Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells [Ｊ]. FEBS Lett， 1996; 382(3)∶271.
5.	Lomo J, Blomhoff HK, Beiske K, et al. TGFbeta 1 and cyclic AMP promote apoptosis in resting human B lymphocytes [Ｊ]. J Immunol, 1995; 154(4)∶1634.
6.	Aharoni D, Dantes A, Oren M, et al. cAMPmediated signals as determinants for apoptosis in primary granulosa cells [Ｊ]. Exp Cell Res, 1995; 218(1)∶271.
7.	Loweth AC, Williams GT, Scarpello JH, et al. Evidence for the involvement of cGMP and protein kinase G in nitric oxideinduced apoptosis in the pancreatic Bcell line, HITT15 [Ｊ]. FEBS Lett, 1997; 400(3)∶285.
8.	Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes [Ｊ]. J Biol Chem, 1997; 272(23)∶14860.
9.	Genaro AM, Hortelano S, Alvarez A, et al. Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl2 levels [Ｊ]. J Clin Invest, 1995; 95(4)∶1884.
10.	Johansen LS. Photodynamic therapy. A new method for the treatment of cancer [Ｊ]. Ugeskr Laeger, 1999; 161(26)∶3992.
11.	Peng Q, Berg K, Moan J, et al. 5Aminolevulinic acidbased photodynamic therapy: principles and experimental research [Ｊ]. Photochem Photobiol, 1997; 65(2)∶235.
12.	Gahlen J, Stern J, Laubach HH, et al. Improving diagnostic staging laparoscopy using intraperitoneal lavage of deltaaminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis [Ｊ]. Surgery, 1999; 126(3)∶469.
13.	陳祖林，唐建民主編. 胃腸腫瘤與現代激光 [M]. 北京：軍事醫學科學出版社，1999∶70～80.
14.	Parvathenani LK, Buescher ES, ChaconCruz E, et al. Type Ⅰ cAMPdependent protein kinase delays apoptosis in human neutrophils at a site upstream of caspase3 [Ｊ]. J Biol Chem, 1998; 273(12)∶6736.
15.	Wolvetang EJ, Johnson KL, Krauer K, et al. Mitochondrial respiratory chain inhibitors induce apoptosis [Ｊ]. FEBS Lett, 1994; 339(1-2)∶40.
16.	Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
17.	GarciaBermejo L, Perez C, Vilaboa NE, et al. cAMP increasing agents attenuate the generation of apoptosis by etoposide in promonocytic leukemia cells [Ｊ]. J Cell Sci, 1998; 111 (Pt 5)∶637.
18.	Penning LC, VanSteveninck J, Dubbelman TM. HPDinduced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24 [Ｊ]. Biochem Biophys Res Commun, 1993; 194(3)∶1084.
19.	Inanami O, Yoshito A, Takahashi K, et al. Effects of BAPTAAM and forskolin on apoptosis and cytochrome c release in photosensitized Chinese hamster V79 cells [Ｊ]. Photochem Photobiol, 1999; 70(4)∶650.

1. Eur J Cancer， 1996; 32A Suppl 5∶S23.
2. Van Cutsem E. A glimpse of the future. New directions in the treatment of colorectal cancer [Ｊ].
3. Almeida RD, Manadas BJ, Carvalho AP, et al. Intracellular signaling mechanisms in photodynamic therapy [Ｊ]. Biochim Biophys Acta， 2004; 1704(2)∶59.
4. Muhl H, Nitsch D, Sandau K, et al. Apoptosis is triggered by the cyclic AMP signalling pathway in renal mesangial cells [Ｊ]. FEBS Lett， 1996; 382(3)∶271.
5. Lomo J, Blomhoff HK, Beiske K, et al. TGFbeta 1 and cyclic AMP promote apoptosis in resting human B lymphocytes [Ｊ]. J Immunol, 1995; 154(4)∶1634.
6. Aharoni D, Dantes A, Oren M, et al. cAMPmediated signals as determinants for apoptosis in primary granulosa cells [Ｊ]. Exp Cell Res, 1995; 218(1)∶271.
7. Loweth AC, Williams GT, Scarpello JH, et al. Evidence for the involvement of cGMP and protein kinase G in nitric oxideinduced apoptosis in the pancreatic Bcell line, HITT15 [Ｊ]. FEBS Lett, 1997; 400(3)∶285.
8. Wu CF, Bishopric NH, Pratt RE. Atrial natriuretic peptide induces apoptosis in neonatal rat cardiac myocytes [Ｊ]. J Biol Chem, 1997; 272(23)∶14860.
9. Genaro AM, Hortelano S, Alvarez A, et al. Splenic B lymphocyte programmed cell death is prevented by nitric oxide release through mechanisms involving sustained Bcl2 levels [Ｊ]. J Clin Invest, 1995; 95(4)∶1884.
10. Johansen LS. Photodynamic therapy. A new method for the treatment of cancer [Ｊ]. Ugeskr Laeger, 1999; 161(26)∶3992.
11. Peng Q, Berg K, Moan J, et al. 5Aminolevulinic acidbased photodynamic therapy: principles and experimental research [Ｊ]. Photochem Photobiol, 1997; 65(2)∶235.
12. Gahlen J, Stern J, Laubach HH, et al. Improving diagnostic staging laparoscopy using intraperitoneal lavage of deltaaminolevulinic acid (ALA) for laparoscopic fluorescence diagnosis [Ｊ]. Surgery, 1999; 126(3)∶469.
13. 陳祖林，唐建民主編. 胃腸腫瘤與現代激光 [M]. 北京：軍事醫學科學出版社，1999∶70～80.
14. Parvathenani LK, Buescher ES, ChaconCruz E, et al. Type Ⅰ cAMPdependent protein kinase delays apoptosis in human neutrophils at a site upstream of caspase3 [Ｊ]. J Biol Chem, 1998; 273(12)∶6736.
15. Wolvetang EJ, Johnson KL, Krauer K, et al. Mitochondrial respiratory chain inhibitors induce apoptosis [Ｊ]. FEBS Lett, 1994; 339(1-2)∶40.
16. Rossi AG, Cousin JM, Dransfield I, et al. Agents that elevate cAMP inhibit human neutrophil apoptosis [Ｊ]. Biochem Biophys Res Commun, 1995; 217(3)∶892.
17. GarciaBermejo L, Perez C, Vilaboa NE, et al. cAMP increasing agents attenuate the generation of apoptosis by etoposide in promonocytic leukemia cells [Ｊ]. J Cell Sci, 1998; 111 (Pt 5)∶637.
18. Penning LC, VanSteveninck J, Dubbelman TM. HPDinduced photodynamic changes in intracellular cyclic AMP levels in human bladder transitional carcinoma cells, clone T24 [Ｊ]. Biochem Biophys Res Commun, 1993; 194(3)∶1084.
19. Inanami O, Yoshito A, Takahashi K, et al. Effects of BAPTAAM and forskolin on apoptosis and cytochrome c release in photosensitized Chinese hamster V79 cells [Ｊ]. Photochem Photobiol, 1999; 70(4)∶650.

《中國普外基礎與臨床雜志》

內源性光動力療法對人結腸癌SW480細胞cAMP和cGMP的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

內源性光動力療法對人結腸癌SW480細胞cAMP和cGMP的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料