CO2氣腹對結腸癌細胞侵襲能力影響的體外研究_《中國普外基礎與臨床雜志》

作者：

馬君俊 , 馮波 , 張軼 , 陸愛國 , 胡偉國 , 李健文 , 王明亮 , 鄭民華

上海交通大學醫學院附屬瑞金醫院普外科，上海市微創外科臨床醫學中心，上海消化外科研究所（上海200025）;

關鍵詞：

結直腸腫瘤人工氣腹細胞黏附分子腫瘤轉移

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目的　研究不同壓強持續性CO2氣腹處理對結腸癌細胞侵襲能力的影響。
方法　建立體外氣腹模型，選用人結腸癌細胞株SW1116，分別在不同壓強（6、9、12及15 mm Hg）99.5%醫用CO2氣體以及常規培養條件下暴露1 h，取各壓強組處理后0 h和72 h細胞，使用體外細胞侵襲實驗模型進行體外侵襲實驗。
結果　氣腹處理后即刻（0 h），15 mm Hg壓強組結腸癌細胞侵襲熒光強度（11.36±0.861)明顯低于處理前（13.43±1.113)，差異有統計學意義（P=0.019），且明顯低于其他各組(6 mm Hg組： 15.58±1.015，P=0.000； 9 mm Hg組： 13.49±0.750, P=0.011; 12 mm Hg組： 13.37±0.949, P=0.016; 常規培養組： 13.43±2.027,P=0.014)； 6 mm Hg壓強組處理后細胞侵襲熒光強度（15.58±1.015)高于處理前（14.42±1.491),但差異尚未達到統計學意義（P=0.056），且顯著高于其他各壓強組（9 mm Hg組： P=0.013; 12 mm Hg組： P=0.009； 15 mm Hg組： P=0.000；常規培養組： P=0.011）。氣腹處理后72 h，各壓強組處理前、后的結腸癌細胞侵襲熒光強度差異無統計學意義（P gt;0.05）；處理后各壓強組間的結腸癌細胞侵襲熒光強度差異亦無統計學意義（P gt;0.05）。
結論　CO2氣腹能對結腸癌細胞的侵襲能力產生一過性影響，隨著CO2氣腹壓強的增高，結腸癌細胞的侵襲能力可能受到抑制。

引用本文： 馬君俊,馮波,張軼,陸愛國,胡偉國,李健文,王明亮,鄭民華. CO2氣腹對結腸癌細胞侵襲能力影響的體外研究. 中國普外基礎與臨床雜志, 2007, 14(5): 512-515. doi: 復制

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1. 　The Clinical Outcomes of Surgical Therapy Study Group. A comparison of laparoscopically assisted and open colectomy for colon cancer [J]. N Engl J Med， 2004; 350(20)∶2050.
2. 　Guillou PJ, Quirke P, Thorpe H, et al. Short-term endpoints of conventional versus laparoscopic-assisted surgery in patients with colorectal cancer (MRC CLASICC trial): multicentre, randomised controlled trial [J]. Lancet， 2005; 365(9472)∶1718.
3. 　Veldkamp R, Kuhry E, Hop WC, et al. Laparoscopic surgery versus open surgery for colon cancer: short-term outcomes of a randomised trial [J]. Lancet Oncol， 2005; 6(7)∶477.
4. 　Yu Q, Stamenkovic I. Localization of matrix metalloproteinase 9 to the cell surface provides a mechanism for CD44-mediated tumor invasion [J]. Genes Dev， 1999; 13(1)∶35.
5. 　鄭民華，馬君俊，馮波，等. CO2氣腹對結腸癌細胞粘附能力影響及其與粘附分子的關系 [J]. 中國實用外科雜志， 2005； 20(11)∶668.
6. 　Liu YJ, Yan PS, Li J, et al. Expression and significance of CD44s, CD44v6, and nm23 mRNA in human cancer [J]. World J Gastroenterol， 2005; 11(42)∶6601.
7. 　Illemann M, Bird N, Majeed A, et al. MMP-9 is differentially expressed in primary human colorectal adenocarcinomas and their metastases [J]. Mol Cancer Res， 2006; 4(5)∶293.
8. 　Li BH, Zhao P, Liu SZ, et al. Matrix metalloproteinase-2 and tissue inhibitor of metallo-proteinase-2 in colorectal carcinoma invasion and metastasis[J]. World J Gastroenterol， 2005; 11(20)∶3046.
9. 　Karadag A, Fedarko NS, Fisher LW. Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44 [J]. Cancer Res， 2005; 65(24)∶11545.
10. 　 Jiang WG. E-cadherin and its associated protein catenins, cancer invasion and metastasis [J]. Br J Surg， 1996; 83(4)∶437.
11. 　Kinsella AR, Green B, Lepts GC, et al. The role of the cell-cell adhesion molecule E-cadherin in large bowel tumour cell invasion and metastasis [J]. Br J Cancer， 1993; 67(5)∶904.
12. 　Wlodarczyk J, Stolte M, Mueller J. E-cadherin, beta-catenin and stromelysin-3 expression in de novo carcinoma of the colorectum [J]. Pol J Pathol， 2001; 52(3)∶119.
13. 　Wlodarczyk J, Bethke B, Mueller E, et al. A comparative study of E-cadherin and stromelysin-3 expression in de novo and ex adenoma carcinoma of the colorectum [J]. Virchows Arch， 2001; 439(6)∶756.
14. 　車向明，王曙逢，樊林，等. E-cadherin反義寡核苷酸對腫瘤細胞浸潤能力影響的研究 [J]. 中國普外基礎與臨床雜志, 2006; 13(2)∶191.
15. 　Zhou YN, Xu CP, Han B, et al. Expression of E-cadherin and beta-catenin in gastric carcinoma and its correlation with the clinicopathological features and patient survival [J]. World J Gastroenterol， 2002; 8(6)∶987.
16. 　Haier J, Nasralla M, Nicolson GL. Cell surface molecules and their prognostic values in assessing colorectal carcinomas [J]. Ann Surg， 2000; 231(1)∶11.
17. 　范西紅，尹格平. 直腸癌和大腸粘膜組織細胞間粘附分子-1的表達及其臨床意義 [J]. 中國普外基礎與臨床雜志， 2000; 7(3)∶155.
18. 　Ziprin P, Ridgway PF, Peck DH, et al. Laparoscopic enhancement of tumour cell binding to the peritoneum is inhibited by anti-intercellular adhesion molecule-1 monoclonal antibody [J]. Surg Endosc， 2003; 17(11)∶1812.
19. 　馬君俊，鄭民華，馮波，等. CO2氣腹對結腸癌細胞表面粘附分子表達影響的研究 [J]. 中華消化內鏡雜志， 2006； 23（2）∶114.
20. 　Gutt CN, Gessmann T, Schemmer P, et al. The impact of carbon dioxide and helium insufflation on experimental liver metastases, macrophages, and cell adhesion molecules [J]. Surg Endosc， 2003; 17(10)∶1628.

《中國普外基礎與臨床雜志》

CO2氣腹對結腸癌細胞侵襲能力影響的體外研究

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《中國普外基礎與臨床雜志》

CO2氣腹對結腸癌細胞侵襲能力影響的體外研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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