乙酰肝素酶在結直腸癌中的表達及意義_《中國普外基礎與臨床雜志》

作者：

肖毅 ¹ , 馮云路 ² , 錢家鳴 ² , 陳原稼 ² , 林國樂 ¹ , 張月寧 ² , 吳斌 ¹ , 邱輝忠 ¹

1.中國醫學科學院北京協和醫院基本外科（北京100730）;;
2.中國醫學科學院北京協和醫院消化內科;

關鍵詞：

乙酰肝素酶結直腸癌預后

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目的　研究乙酰肝素酶（HPA1）在結直腸癌中的表達，探討其與腫瘤生長和預后的關系。
方法　采用RT-PCR和免疫組織化學技術，從mRNA和蛋白水平對HPA1在結直腸癌及其周圍組織中的表達進行定性和定位研究，初步分析其與結直腸癌臨床病理特征和臨床預后的關系。
結果　HPA1 mRNA在結直腸癌組織中的表達明顯高于周圍正常組織，HPA1 mRNA在正常腸道組織中的表達與是否患結直腸腺瘤呈正相關（r=0.352，P=0.024）；免疫組化結果發現，HPA1蛋白主要表達在結直腸癌組織、腫瘤邊緣的浸潤組織和血管內皮細胞； HPA1陰性患者的2年無病生存率（88.9%）高于HPA1陽性患者（50.0%），但差異無統計學意義（P=0.078）。
結論　HPA1在結直腸癌組織中呈高表達，與腫瘤的發生和預后有一定的關系。

引用本文： 肖毅,馮云路,錢家鳴,陳原稼,林國樂,張月寧,吳斌,邱輝忠. 乙酰肝素酶在結直腸癌中的表達及意義. 中國普外基礎與臨床雜志, 2007, 14(4): 442-447. doi: 復制

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2.	Sanderson RD. Heparan sulfate proteoglycans in invasion and metastasis [Ｊ]　. Semin Cell Dev Biol, 2001; 12(2)∶89.
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4.	Elkin M, Ilan N, Ishai-Michaeli R, et al. Heparanase as mediator of angiogenesis: mode of action [Ｊ]　. FASEB J, 2001; 15(9)∶1661　.
5.	Greene FL, Page DL, Fleming ID, et al. AJCC Cancer Staging Manual [M]　. 6th Edition. New York: Springer, 2002　∶113-119　.
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7.	Koliopanos A, Friess H, Kleeff J, et al. Heparanase expression in primary and metastatic pancreatic cancer [Ｊ]　. Cancer Res, 2001; 61(12)∶4655.
8.	Takaoka M, Naomoto Y, Ohkawa T, et al. Heparanase expression correlates with invasion and poor prognosis in gastric cancers [Ｊ]　. Lab Invest, 2003; 83(5)∶613.
9.	Xiao Y, Kleeff J, Shi X, et al. Heparanase expression in hepatocellular carcinoma and the cirrhotic liver [Ｊ]　. Hepatol Res, 2003; 26(3)∶192.
10.	Stadlmann S, Moser PL, Pollheimer J, et al. Heparanase-1 gene expression in normal, hyperplastic and neoplastic prostatic tissue [Ｊ]　. Eur J Cancer, 2003; 39(15)∶2229.
11.	Hulett MD, Freeman C, Hamdorf BJ, et al. Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis [Ｊ]　. Nat Med, 1999; 5(7)∶803.
12.	Nobuhisa T, Naomoto Y, Ohkawa T, et al. Heparanase expression correlates with malignant potential in human colon cancer [Ｊ]　. J Cancer Res Clin Oncol, 2005; 131(4)∶229.
13.	Friedmann Y, Vlodavsky I, Aingorn H, et al. Expression of heparanase in normal, dysplastic, and neoplastic human colonic mucosa and stroma. Evidence for its role in colonic tumorigenesis [Ｊ]　. Am J Pathol, 2000; 157(4)∶1167.
14.	Edovitsky E, Elkin M, Zcharia E, et al. Heparanase gene silencing, tumor invasiveness, angiogenesis, and metastasis [Ｊ]　. J Natl Cancer Inst， 2004; 96(16)∶1219.
15.	Sato T, Yamaguchi A, Goi T, et al. Heparanase expression in human colorectal cancer and its relationship to tumor angiogenesis, hematogenous metastasis, and prognosis [Ｊ]　. J Surg Oncol, 2004; 87(4)∶174.
16.	Wang ZN, Xu HM, Jiang L, et al. Positive association of Rh-oC gene overexpression with tumour invasion and lymphatic metastasis in gastric carcinoma [Ｊ]　. Chin Med J (Engl), 2005; 118(6)∶502.

1. 　Bernfield M, Gotte M, Park PW, et al. Functions of cell surface heparan sulfate proteoglycans [Ｊ]　. Annu Rev Biochem, 1999; 68∶729.
2. 　Sanderson RD. Heparan sulfate proteoglycans in invasion and metastasis [Ｊ]　. Semin Cell Dev Biol, 2001; 12(2)∶89.
3. 　Kleeff J, Ishiwata T, Kumbasar A, et al. The cell-surface heparan sulfate proteoglycan glypican-1 regulates growth factor action in pancreatic carcinoma cells and is overexpressed in human pancreatic cancer [Ｊ]　. J Clin Invest, 1998; 102(9)∶1662.
4. 　Elkin M, Ilan N, Ishai-Michaeli R, et al. Heparanase as mediator of angiogenesis: mode of action [Ｊ]　. FASEB J, 2001; 15(9)∶1661　.
5. 　Greene FL, Page DL, Fleming ID, et al. AJCC Cancer Staging Manual [M]　. 6th Edition. New York: Springer, 2002　∶113-119　.
6. 　Vlodavsky I, Friedmann Y, Elkin M, et al. Mammalian hepa-ranase: gene cloning, expression and function in tumor progression and metastasis [Ｊ]　. Nat Med, 1999; 5(7)∶793.
7. 　Koliopanos A, Friess H, Kleeff J, et al. Heparanase expression in primary and metastatic pancreatic cancer [Ｊ]　. Cancer Res, 2001; 61(12)∶4655.
8. 　Takaoka M, Naomoto Y, Ohkawa T, et al. Heparanase expression correlates with invasion and poor prognosis in gastric cancers [Ｊ]　. Lab Invest, 2003; 83(5)∶613.
9. 　Xiao Y, Kleeff J, Shi X, et al. Heparanase expression in hepatocellular carcinoma and the cirrhotic liver [Ｊ]　. Hepatol Res, 2003; 26(3)∶192.
10. 　Stadlmann S, Moser PL, Pollheimer J, et al. Heparanase-1 gene expression in normal, hyperplastic and neoplastic prostatic tissue [Ｊ]　. Eur J Cancer, 2003; 39(15)∶2229.
11. 　Hulett MD, Freeman C, Hamdorf BJ, et al. Cloning of mammalian heparanase, an important enzyme in tumor invasion and metastasis [Ｊ]　. Nat Med, 1999; 5(7)∶803.
12. 　Nobuhisa T, Naomoto Y, Ohkawa T, et al. Heparanase expression correlates with malignant potential in human colon cancer [Ｊ]　. J Cancer Res Clin Oncol, 2005; 131(4)∶229.
13. 　Friedmann Y, Vlodavsky I, Aingorn H, et al. Expression of heparanase in normal, dysplastic, and neoplastic human colonic mucosa and stroma. Evidence for its role in colonic tumorigenesis [Ｊ]　. Am J Pathol, 2000; 157(4)∶1167.
14. 　Edovitsky E, Elkin M, Zcharia E, et al. Heparanase gene silencing, tumor invasiveness, angiogenesis, and metastasis [Ｊ]　. J Natl Cancer Inst， 2004; 96(16)∶1219.
15. 　Sato T, Yamaguchi A, Goi T, et al. Heparanase expression in human colorectal cancer and its relationship to tumor angiogenesis, hematogenous metastasis, and prognosis [Ｊ]　. J Surg Oncol, 2004; 87(4)∶174.
16. 　Wang ZN, Xu HM, Jiang L, et al. Positive association of Rh-oC gene overexpression with tumour invasion and lymphatic metastasis in gastric carcinoma [Ｊ]　. Chin Med J (Engl), 2005; 118(6)∶502.

《中國普外基礎與臨床雜志》

乙酰肝素酶在結直腸癌中的表達及意義

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《中國普外基礎與臨床雜志》

乙酰肝素酶在結直腸癌中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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