人血管內皮抑素畢赤酵母真核系統的建立_《中國普外基礎與臨床雜志》

作者：

周東風 ¹ , 張忠廣 ²

1.青島市市立醫院普外科（青島266011）;;
2.青島大學醫學院病原生物教研室（青島266011）;

關鍵詞：

人血管內皮抑素克隆構建酵母

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　構建人血管內皮抑素（ES）畢赤酵母真核表達系統。
方法　利用RT-PCR技術從人體肝臟組織擴增出ES基因，插入畢赤酵母表達載體pPIC9中，酶切鑒定并進行DNA測序鑒定。
結果　成功克隆ES基因并篩選出pPIC9載體中的ES陽性克隆，測序證實其序列與已報道的ES基因序列一致。
結論　用分子生物學方法構建ES畢赤酵母真核表達載體的成功，使高效表達ES蛋白成為可能，并為采用抗血管生成方法治療惡性腫瘤的研究奠定了基礎。

引用本文： 周東風,張忠廣. 人血管內皮抑素畢赤酵母真核系統的建立. 中國普外基礎與臨床雜志, 2007, 14(4): 417-419. doi: 復制

1.	O’Reilly MS, Boehm T, Shing Y, et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth [Ｊ]　. Cell, 1997; 88(2)∶277.
2.	Davis DW, Shen Y, Mullani NA, et al. Quantitative analysis of biomarkers defines an optimal biological dose for recombinant human endostatin in primary human tumors [Ｊ]　. Clin Cancer Res, 2004; 10(1 Pt 1)∶33.
3.	Capillo M, Mancuso P, Gobbi A, et al. Continuous infusion of endostatin inhibits differentiation, mobilization, and clonogenic potential of endothelial cell progenitors [Ｊ]　. Clin Cancer Res, 2003; 9(1)∶377.
4.	賀祥，潘衛，黃耀誠，等. 血管生成抑制素Endostatin研究新進展 [Ｊ]　. 中國腫瘤生物治療雜志， 2000； 7（1）∶76.
5.	楊林，王金萬，湯仲明，等. 重組人血管內皮抑制素Ⅰ期臨床研究 [Ｊ]　. 中國新藥研究， 2004； 13（6）∶538.
6.	Boehm T, Folkman J, Browder T, et al. Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance [Ｊ]　. Nature, 1997; 390(6658)∶404.
7.	Nyberg P, Heikkila P, Sorsa T, et al. Endostatin inhibits human tongue carcinoma cell invasion and intravasation and blocks the activation of matrix metalloprotease-2, -9, and -13 [Ｊ]　. J Biol Chem, 2003; 278(25)∶22404.
8.	劉然義，易繼林，邵俊偉，等. 內皮抑素轉基因治療裸鼠人肝癌的實驗研究[Ｊ]. 中國普外基礎與臨床雜志， 2006； 13（2）∶145.
9.	Hansma AH, Broxterman HJ, van der Horst I, et al. Recombinant human endostatin administered as a 28-day continuous intravenous infusion, followed by daily subcutaneous injections: a phase Ⅰ and pharmacokinetic study in patients with advanced cancer [Ｊ]　. Ann Oncol， 2005; 16(10)∶1695.
10.	Minning S, Serrano A, Ferrer P, et al. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris [Ｊ]　. J Biotechnol， 2001; 86(1)∶59.
11.	Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris [Ｊ]　. FEMS Microbiol Rev， 2000; 24(1)∶45.

1. 　O’Reilly MS, Boehm T, Shing Y, et al. Endostatin: an endogenous inhibitor of angiogenesis and tumor growth [Ｊ]　. Cell, 1997; 88(2)∶277.
2. 　Davis DW, Shen Y, Mullani NA, et al. Quantitative analysis of biomarkers defines an optimal biological dose for recombinant human endostatin in primary human tumors [Ｊ]　. Clin Cancer Res, 2004; 10(1 Pt 1)∶33.
3. 　Capillo M, Mancuso P, Gobbi A, et al. Continuous infusion of endostatin inhibits differentiation, mobilization, and clonogenic potential of endothelial cell progenitors [Ｊ]　. Clin Cancer Res, 2003; 9(1)∶377.
4. 　賀祥，潘衛，黃耀誠，等. 血管生成抑制素Endostatin研究新進展 [Ｊ]　. 中國腫瘤生物治療雜志， 2000； 7（1）∶76.
5. 　楊林，王金萬，湯仲明，等. 重組人血管內皮抑制素Ⅰ期臨床研究 [Ｊ]　. 中國新藥研究， 2004； 13（6）∶538.
6. 　Boehm T, Folkman J, Browder T, et al. Antiangiogenic therapy of experimental cancer does not induce acquired drug resistance [Ｊ]　. Nature, 1997; 390(6658)∶404.
7. 　Nyberg P, Heikkila P, Sorsa T, et al. Endostatin inhibits human tongue carcinoma cell invasion and intravasation and blocks the activation of matrix metalloprotease-2, -9, and -13 [Ｊ]　. J Biol Chem, 2003; 278(25)∶22404.
8. 　劉然義，易繼林，邵俊偉，等. 內皮抑素轉基因治療裸鼠人肝癌的實驗研究[Ｊ]. 中國普外基礎與臨床雜志， 2006； 13（2）∶145.
9. 　Hansma AH, Broxterman HJ, van der Horst I, et al. Recombinant human endostatin administered as a 28-day continuous intravenous infusion, followed by daily subcutaneous injections: a phase Ⅰ and pharmacokinetic study in patients with advanced cancer [Ｊ]　. Ann Oncol， 2005; 16(10)∶1695.
10. 　Minning S, Serrano A, Ferrer P, et al. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris [Ｊ]　. J Biotechnol， 2001; 86(1)∶59.
11. 　Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris [Ｊ]　. FEMS Microbiol Rev， 2000; 24(1)∶45.

《中國普外基礎與臨床雜志》

人血管內皮抑素畢赤酵母真核系統的建立

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

人血管內皮抑素畢赤酵母真核系統的建立

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料