重組腺病毒介導hTIMP-1對人肝癌細胞系體外增殖的影響_《中國普外基礎與臨床雜志》

作者：

夏冬 ¹ , 徐亮 ² , 萬禮儀 ² , 王元正 ² , 左懷全 ¹ , 嚴律南 ¹

1.四川大學華西醫院普外科（成都610041）;;
2.四川瀘州醫學院附屬醫院普外科（瀘州646000）;

關鍵詞：

基質金屬蛋白酶組織抑制因子肝細胞癌腺病毒基因治療

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目的　探討人基質金屬蛋白酶組織抑制因子-1（hTIMP-1）過表達對人肝癌細胞系HepG2體外增殖的作用。
方法　構建攜載hTIMP-1全長cDNA的重組腺病毒載體，感染HepG2細胞，應用Western blot及RT-PCR方法檢測TIMP-1蛋白及mRNA的表達，透射電鏡觀察細胞超微結構改變，MTT法檢測hTIMP-1對HepG2細胞生長的影響。
結果　成功構建攜載hTIMP-1的重組腺病毒載體，感染HepG2細胞，實現其體外穩定表達，hTIMP-1過表達對HepG2體外增殖有明顯抑制作用。
結論　hTIMP-1過表達可抑制人肝癌細胞系HepG2的體外增殖，可用于肝癌基因治療的研究。

引用本文： 夏冬,徐亮,萬禮儀,王元正,左懷全,嚴律南. 重組腺病毒介導hTIMP-1對人肝癌細胞系體外增殖的影響. 中國普外基礎與臨床雜志, 2007, 14(4): 409-412. doi: 復制

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1. 　夏冬，嚴律南． MMPs-TIMPs與肝癌轉移 [Ｊ]　. 中華肝膽外科雜志， 2005; 11(8)∶574.
2. 　He TC, Zhou S, da Costa LT, et al. A simplified system for generating recombinant adenovirus [Ｊ]　. Proc Natl Acad Sci USA, 1998; 95(12)∶2509.
3. 　Xia D, Yan LN, Tong Y, et al. Construction of recombinant adenoviral vector carrying human tissue inhibitor of metalloproteinase-1 gene and its expression in vitro [Ｊ]　. Hepat Pancrea Dis International, 2005; 4(2)∶259.
4. 　Matsumoto E, Nakatsukasa H, Nouso K, et al. Increased levels of tissue inhibitor of metalloproteinase-1 in human hepatocellular carcinoma [Ｊ]　. Liver Int, 2004; 24(4)∶379.
5. 　Rigg AS, Lemoine NR. Adenoviral delivery of TIMP-1 or TI-MP-2 can modify the invasive behavior of pancreatic cancer and can have a significant antitumor effect in vitro [Ｊ]　. Cancer Gene Ther, 2001; 8(3)∶869.
6. 　Xia D, Zhang MM, Yan LN. Recent advances in liver-directed gene transfer vectors [Ｊ]　. Hepat Pancrea Dis International, 2004; 3(3)∶332.
7. 　Tran PL, Vigneron JP, Pericat D, et al. Gene therapy for hepatocellular carcinoma using non-viral vectors composed of bis guanidinium-tren-cholesterol and plasmids encoding the tissue inhibitors of metalloproteinases TIMP-1 and TIMP-3 [Ｊ]　. Cancer Gene Ther, 2003; 10（6）∶435.
8. 　Kim JR, Kim CH. Association of a high activity of matrix metalloproteinase-9 to low levels of tissue inhibitors of metalloproteinase-1 and -3 in human hepatitis B-viral hepatoma cells [Ｊ]　. Int J Biochem Cell Biol, 2004; 36（4）∶2293.
9. 　Ogasawara S, Yano H, Momosaki S, et al. Expression of matrix metalloproteinases (MMPs) in cultured epatocellular carcinoma (HCC) cells and surgically resected HCC tissues [Ｊ]　. Oncol Rep, 2005; 13（6）∶1043.
10. 　Egeblad M, Werb Z. New functions for the matrix metalloproteinases in cancer progression [Ｊ]　. Nat Rev Cancer, 2002; 2（3）∶163　.
11. 　Jiang YF, Goldberg ID, Shi YE. Complex roles of tissue inhibitors of metalloproteinase in cancer [Ｊ]　. Oncogene, 2002; 21（14）∶2245.
12. 　Li H, Lindenmeyer F, Grenet C, et al. AdTIMP-2 inhibits tumor growth, angiogenesis, and metastasis, and prolongs survival in mice [Ｊ]　. Hum Gene Ther, 2001; 12(5)∶515.

《中國普外基礎與臨床雜志》

重組腺病毒介導hTIMP-1對人肝癌細胞系體外增殖的影響

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《中國普外基礎與臨床雜志》

重組腺病毒介導hTIMP-1對人肝癌細胞系體外增殖的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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