103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究_《中國普外基礎與臨床雜志》

作者：

何貴金 ¹ , 于發強 ¹ , 高沁怡 ² , 許書河 ³ , 高紅 ⁴ , 姜維國 ⁵ , 蔣濤 ⁴ , 戴顯偉 ¹ , 戴朝六 ¹

1.中國醫科大學盛京醫院肝膽外科（沈陽110004）;;
2.中國醫科大學第一臨床學院附院核醫學科（沈陽110001）;;
3.中國原子能研究院同位素研究所（北京100243）;;
4.中國醫科大學盛京醫院中心實驗室;;
5.中國醫科大學盛京醫院病理科;

關鍵詞：

103Pd放射性支架 γ射線 Fas基因細胞凋亡

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目的　探討103鈀（103Pd）放射性支架釋放的γ射線對Fas表達的影響以及與膽管癌細胞凋亡的關系及意義。
方法　培養瓶中膽管癌細胞消化后形成細胞懸液，采用血細胞計數法計數細胞，按1×105/ml的濃度分裝到2 ml塑料凍存管中。分別設置普通支架組及103Pd支架組，應用TUNEL法和免疫組化染色方法檢測γ射線誘導膽管癌細胞凋亡的情況和Fas表達的情況。
結果　103Pd支架組膽管癌細胞的Fas表達較普通支架組明顯增高（P＜0.05），且膽管癌細胞出現明顯細胞凋亡，凋亡細胞數明顯高于普通支架組（P＜0.01）。
結論　Fas表達水平與膽管癌細胞凋亡的發生有關，103Pd放射性支架可能通過增強Fas表達，促進膽管癌細胞凋亡，從而為臨床應用103Pd放射性支架治療膽管癌提供理論依據。

引用本文： 何貴金,于發強,高沁怡,許書河,高紅,姜維國,蔣濤,戴顯偉,戴朝六. 103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究. 中國普外基礎與臨床雜志, 2007, 14(3): 284-286. doi: 復制

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1. Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiation-induced apoptosis by specific expression of Bcl-2 in normal cells [J]. Cancer Gene Ther， 2006; 13(5)∶451.
2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J， 2004; 10(3)∶170.
3. Grigoriu M, Palade R, Vasile D, et al. Technic, indications and contribution of choledochoscopy in the diagnosis and treatment of biliary diseases [J]. Chirurgia (Bucur)， 2003; 98(2)∶167.
4. Wiedermann JG, Marboe C, Amols H, et al. Intracoronary irradiation markedly reduces restenosis after balloon angioplasty in a porcine model [J]. J Am Coll Cardiol， 1994; 23(6)∶1491.
5. 李海民，竇科峰，李韌, 等. 肝癌組織中Fas和FasL mRNA表達水平的變化及其意義 [J]. 中國普外基礎與臨床雜志, 2005; 12（3）∶284.
6. Bittner RM, Crowe SF. The relationship between naming difficulty and FAS performance following traumatic brain injury [J]. Brain Inj， 2006; 20(9)∶971.
7. Drewa T, Wolski Z, Skok Z, et al. The FAS-related apoptosis signaling pathway in the prostate intraepithelial neoplasia and cancer lesions [J]. Acta Pol Pharm， 2006; 63(4)∶311.
8. Protonotariou E, Rizos D, Malamitsi-Puchner A, et al. Tissue polypeptide specific antigen and soluble Fas during normal pregnancy and early life [J]. In Vivo， 2006; 20(6B)∶901.
9. McVicker BL, Tuma DJ, Kubik JL, et al. Ethanol-induced apoptosis in polarized hepatic cells possibly through regulation of the Fas pathway [J]. Alcohol Clin Exp Res， 2006; 30(11)∶1906.
10. Weissman JL. Old problems, new techniques: the interventional radiologist and the lacrimal apparatus [J]. Radiology， 1999; 212(2)∶305.
11. Oshimi Y, Miyazaki S. Fas antigen-mediated DNA fragmentation and apoptotic morphologic changes are regulated by elevated cytosolic Ca2+ level [J]. J Immunol， 1995; 154(2)∶599.
12. Venet F, Pachot A, Debard AL, et al. Human CD4+CD25+ regulatory T lymphocytes inhibit lipopolysaccharide-induced monocyte survival through a Fas/Fas ligand-dependent mechanism [J]. J Immunol， 2006; 177(9)∶6540.
13. Chmura SJ, Nodzenski E, Beckett MA, et al. Loss of ceramide production confers resistance to radiation-induced apoptosis [J]. Cancer Res， 1997; 57(7)∶1270.
14. Gadbois DM, Crissman HA, Nastasi A, et al. Alterations in the progression of cells through the cell cycle after exposure to alpha particles or gamma rays [J]. Radiat Res， 1996; 146(4)∶414.
15. Petri S, Kiaei M, Wille E, et al. Loss of Fas ligand-function improves survival in G93A-transgenic ALS mice [J]. J Neurol Sci， 2006; 251(1-2)∶44.

《中國普外基礎與臨床雜志》

103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究

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《中國普外基礎與臨床雜志》

103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料