直腸癌及其遠端黏膜P53和CD34的表達及意義_《中國普外基礎與臨床雜志》

作者：

涂小煌 ¹ , 王為忠 ² , 王烈 ¹ , 黎成金 ¹ , 宋京翔 ¹ , 宋麗娜 ¹ , 趙春雷 ¹

1.南京軍區福州總醫院普通外科研究所（福州350025）;;
2.第四軍醫大學附屬西京醫院胃腸外科（西安710032）;

關鍵詞：

直腸癌全直腸系膜切除術 P53 CD34 PET/CT 免疫組織化學

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目的　觀察直腸癌組織及其遠端黏膜組織中P53蛋白表達及微血管分布，并從分子病理學水平探討直腸癌的安全遠側切緣。
方法　對45例中低位直腸癌下緣進行標記，PET/CT檢查并結合半定量免疫組織化學方法檢測直腸癌及其遠端黏膜組織中P53和CD34蛋白的表達。
結果　直腸癌及其遠端黏膜組織中均可見P53蛋白表達及微血管分布，直腸癌組織中P53蛋白表達及微血管密度(MVD)明顯高于直腸癌遠端黏膜組織(P＜0.01)。直腸癌遠端黏膜組織中P53蛋白表達及MVD隨著距離增大而減少，但有統計學意義的改變只在直腸癌遠端1.5 cm內。正常直腸組織中仍有P53蛋白表達和微血管分布。除直腸癌組織及其遠端0.5 cm段黏膜組織中MVD與腫瘤直徑有關(P＜0.05)外，直腸癌及其遠端黏膜組織中P53蛋白表達及MVD分布與腫瘤直徑、分期以及分化程度均無關。
結論　從分子病理學角度說明2 cm的直腸癌遠端長度是安全的遠側切緣。

引用本文： 涂小煌,王為忠,王烈,黎成金,宋京翔,宋麗娜,趙春雷. 直腸癌及其遠端黏膜P53和CD34的表達及意義. 中國普外基礎與臨床雜志, 2008, 15(9): 641-645. doi: 復制

1.	Jeong SY, Chessin DB, Guillem JG. Surgical treatment of rectal cancer: radical resection [Ｊ]. Surg Oncol Clin N Am， 2006; 15(1)∶95.
2.	涂小煌，王烈主編. 直腸癌全直腸系膜切除術 [M]. 第1版. 福州：福建科學技術出版社, 2006∶122～130.
3.	Munro AJ, Lain S, Lane DP. P53 abnormalities and outcomes in colorectal cancer: a systematic review [Ｊ]. Br J Cancer， 2005; 92(3)∶434.
4.	孫治君, 周鑫, 姚榛祥, 等. P-gp、nm23和p53聯合檢測判斷乳腺癌預后的價值 [Ｊ]. 中國普外基礎與臨床雜志, 2005; 12(3)∶244.
5.	Hilska M, Collan YU, O Laine VJ, et al. The significance of tumor markers for proliferation and apoptosis in predicting survival in coloretal cancer [Ｊ]. Dis Colon Rectum, 2005; 48(12)∶2197.
6.	Zhou XD, Yu JP, Chen HX, et al. Expression of cellular FLICE-inhibitory protein and its association with p53 mutation in colon cancer [Ｊ]. World J Gastroenterol, 2005; 11(16)∶2482.
7.	Salmon JS, Lockhart AC, Berlin J. Anti-angiogenic treatment of gastrointestinal malignancies [Ｊ]. Cancer Invest, 2005; 23(8)∶712.
8.	Uzzan B, Nicolas P, Cucherat M, et al. Microvessel density as a prognostic factor in women with breast cancer: a systematic review of the literature and meta-analysis [Ｊ]. Cancer Res, 2004; 64(9)∶2941.
9.	張劍, 何生, 李茂德. 肝細胞癌中iNOS和p53蛋白的表達及其與腫瘤血管形成的關系 [Ｊ]. 中國普外基礎與臨床雜志, 2004; 11(5)∶396.
10.	Famulski W, Sulkowska M, Wincewicz A, et al. P53 correlates positively with VEGF in preoperative sera of colorectal cancer patients [Ｊ]. Neoplasma, 2006; 53(1)∶43.
11.	Kern A, Taubert H, Scheele J, et al. Association of p53 mutations, microvessel density and neoangiogenesis in pairs of colorectal cancers and corresponding liver metastases [Ｊ]. Int J Oncol, 2002; 21(2)∶243.
12.	Boxer GM, Tsiompanou E, Levine T, et al. Immunohistochemical expression of vascular endothelial growth factor and microvessel counting as prognostic indicators in node-negative colorectal cancer [Ｊ]. Tumour Biol, 2005; 26(1)∶1.
13.	Lebe B, Sarioglu S, Skmen S, et al. The clinical significance of p53, p21, and p27 expressions in rectal carcinoma [Ｊ]. Appl Immunohistochem Mol Morphol, 2005; 13(1)∶38.
14.	Ngoi SS, Staiano-Coico L, Godwin TA, et al. Abnormal DNA ploidy and proliferative patterns in superficial colonic epithelium adjacent to colorectal cancer [Ｊ]. Cancer, 1990; 66(5)∶953.
15.	潘志忠, 萬德森, 張昌卿, 等. 大切片P53免疫組化染色對判斷直腸癌遠端擴散長度的臨床意義 [Ｊ]. 癌癥, 2004; 23(10)∶1199.
16.	范恩學, 孫海俠, 李愛麗. 直腸癌旁移行黏膜P21 P53蛋白表達及其對保肛術的影響 [Ｊ]. 山西醫藥雜志, 2005; 34(8)∶667.
17.	鄭國強, 王怡, 陳香玲, 等. 直腸癌Dixon術后直腸下端切緣p53突變基因分析 [Ｊ]. 臨床腫瘤學雜志, 2000; 5(4)∶229.
18.	鄧海軍, 李國新, 卿三華, 等. 直腸癌遠側癌旁黏膜p53基因突變及其蛋白表達 [Ｊ]. 實用癌癥雜志, 2003; 18(2)∶170.
19.	Heald RJ, Husband EM, Ryall RD. The mesorectum in rectal cancer surgery——the clue to pelvic recurrence? [Ｊ]. Br J Surg, 1982; 69(10)∶613.
20.	Karanjia ND, Schache DJ, North WR, et al. ‘Close shave’ in anterior resection [Ｊ]. Br J Surg, 1990; 77(5)∶510.

1. 　Jeong SY, Chessin DB, Guillem JG. Surgical treatment of rectal cancer: radical resection [Ｊ]. Surg Oncol Clin N Am， 2006; 15(1)∶95.
2. 　涂小煌，王烈主編. 直腸癌全直腸系膜切除術 [M]. 第1版. 福州：福建科學技術出版社, 2006∶122～130.
3. 　Munro AJ, Lain S, Lane DP. P53 abnormalities and outcomes in colorectal cancer: a systematic review [Ｊ]. Br J Cancer， 2005; 92(3)∶434.
4. 　孫治君, 周鑫, 姚榛祥, 等. P-gp、nm23和p53聯合檢測判斷乳腺癌預后的價值 [Ｊ]. 中國普外基礎與臨床雜志, 2005; 12(3)∶244.
5. 　Hilska M, Collan YU, O Laine VJ, et al. The significance of tumor markers for proliferation and apoptosis in predicting survival in coloretal cancer [Ｊ]. Dis Colon Rectum, 2005; 48(12)∶2197.
6. 　Zhou XD, Yu JP, Chen HX, et al. Expression of cellular FLICE-inhibitory protein and its association with p53 mutation in colon cancer [Ｊ]. World J Gastroenterol, 2005; 11(16)∶2482.
7. 　Salmon JS, Lockhart AC, Berlin J. Anti-angiogenic treatment of gastrointestinal malignancies [Ｊ]. Cancer Invest, 2005; 23(8)∶712.
8. 　Uzzan B, Nicolas P, Cucherat M, et al. Microvessel density as a prognostic factor in women with breast cancer: a systematic review of the literature and meta-analysis [Ｊ]. Cancer Res, 2004; 64(9)∶2941.
9. 　張劍, 何生, 李茂德. 肝細胞癌中iNOS和p53蛋白的表達及其與腫瘤血管形成的關系 [Ｊ]. 中國普外基礎與臨床雜志, 2004; 11(5)∶396.
10. 　Famulski W, Sulkowska M, Wincewicz A, et al. P53 correlates positively with VEGF in preoperative sera of colorectal cancer patients [Ｊ]. Neoplasma, 2006; 53(1)∶43.
11. 　Kern A, Taubert H, Scheele J, et al. Association of p53 mutations, microvessel density and neoangiogenesis in pairs of colorectal cancers and corresponding liver metastases [Ｊ]. Int J Oncol, 2002; 21(2)∶243.
12. 　Boxer GM, Tsiompanou E, Levine T, et al. Immunohistochemical expression of vascular endothelial growth factor and microvessel counting as prognostic indicators in node-negative colorectal cancer [Ｊ]. Tumour Biol, 2005; 26(1)∶1.
13. 　Lebe B, Sarioglu S, Skmen S, et al. The clinical significance of p53, p21, and p27 expressions in rectal carcinoma [Ｊ]. Appl Immunohistochem Mol Morphol, 2005; 13(1)∶38.
14. 　Ngoi SS, Staiano-Coico L, Godwin TA, et al. Abnormal DNA ploidy and proliferative patterns in superficial colonic epithelium adjacent to colorectal cancer [Ｊ]. Cancer, 1990; 66(5)∶953.
15. 　潘志忠, 萬德森, 張昌卿, 等. 大切片P53免疫組化染色對判斷直腸癌遠端擴散長度的臨床意義 [Ｊ]. 癌癥, 2004; 23(10)∶1199.
16. 　范恩學, 孫海俠, 李愛麗. 直腸癌旁移行黏膜P21 P53蛋白表達及其對保肛術的影響 [Ｊ]. 山西醫藥雜志, 2005; 34(8)∶667.
17. 　鄭國強, 王怡, 陳香玲, 等. 直腸癌Dixon術后直腸下端切緣p53突變基因分析 [Ｊ]. 臨床腫瘤學雜志, 2000; 5(4)∶229.
18. 　鄧海軍, 李國新, 卿三華, 等. 直腸癌遠側癌旁黏膜p53基因突變及其蛋白表達 [Ｊ]. 實用癌癥雜志, 2003; 18(2)∶170.
19. 　Heald RJ, Husband EM, Ryall RD. The mesorectum in rectal cancer surgery——the clue to pelvic recurrence? [Ｊ]. Br J Surg, 1982; 69(10)∶613.
20. 　Karanjia ND, Schache DJ, North WR, et al. ‘Close shave’ in anterior resection [Ｊ]. Br J Surg, 1990; 77(5)∶510.

《中國普外基礎與臨床雜志》

直腸癌及其遠端黏膜P53和CD34的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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《中國普外基礎與臨床雜志》

直腸癌及其遠端黏膜P53和CD34的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料