散發性結直腸癌染色體7q21－22區雜合性缺失精細定位_《中國普外基礎與臨床雜志》

作者：

許世峰 ¹ , 徐延田 ¹ , 彭志海 ²

1.山東省立醫院普外科(濟南250021)2.上海市第一人民醫院普外科(上海200080);

關鍵詞：

散發性結直腸癌雜合性缺失精細定位

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目的　研究散發性結直腸癌7號染色體雜合性缺失,對7q21－22區精細定位,尋找新的結直腸癌抑癌基因。
方法　采用15對微衛星DNA標記7號染色體,在高頻雜合缺失區另取5對微衛星標記對83例結直腸癌病例的腫瘤和正常組織進行PCR反應。PCR產物在ABI Prism 377自動熒光測序儀進行電泳3 h，以GeneScan 3.1和Genotyper 2.1軟件進行基因分型。
結果　在7號染色體上發現1個高頻雜合缺失區即7q21－22區。對該區再用5對微衛星標記引物行精細定位，界定了1個跨越D7S657、D7S646位點精細的高頻雜合缺失區域。
結論　通過精細雜合缺失作圖的研究，在7號染色體發現了1個跨越D7S657、D7S646位點的精細雜合缺失區，該區很可能存在1個或多個與結直腸癌相關的新的抑癌基因。

引用本文： 許世峰,徐延田,彭志海. 散發性結直腸癌染色體7q21－22區雜合性缺失精細定位. 中國普外基礎與臨床雜志, 2008, 15(9): 637-641. doi: 復制

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2.	駱成玉，祝學光，李世擁, 等. nm23H1基因雜合性缺失及突變與大腸癌轉移抑制 [J]. 中國普外基礎與臨床雜志, 1999； 6(3)∶143.
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5.	van Dieren JM, Wink JC, Vissers KJ, et al. Chromosomal and microsatellite instability of adenocarcinomas and dysplastic lesions (DALM) in ulcerative colitis [J]. Diagn Mol Pathol, 2006; 15(4)∶216.
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7.	Goodison S, Viars C, Urquidi V. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotypespecific chromosomal rearrangements [Ｊ]. Cancer Genet Cytogenet, 2005; 156(1)∶37.
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10.	Trovato M, Ulivieri A, Dominici R, et al. Clinicopathological significance of celltypespecific loss of heterozygosity on chromosome 7q21: analysis of 318 microdissected thyroid lesions [Ｊ]. Endocr Relat Cancer, 2004; 11(2)∶365.
11.	Trovato M, Fraggetta F, Villari D, et al. Loss of heterozygosity of the long arm of chromosome 7 in follicular and anaplastic thyroid cancer, but not in papillary thyroid cancer [Ｊ]. J Clin Endocrinol Metab, 1999; 84(9)∶3235.
12.	Vanharanta S, Wortham NC, Langford C, et al. Definition of a minimal region of deletion of chromosome 7 in uterine leiomyomas by tilingpath microarray CGH and mutation analysis of known genes in this region [Ｊ]. Genes Chromosomes Cancer, 2007; 46(5)∶451.
13.	Neville PJ, Thomas N, Campbell IG. Loss of heterozygosity at 7q22 and mutation analysis of the CDP gene in human epithelial ovarian tumors [Ｊ]. Int J Cancer, 2001; 91(3)∶345.
14.	Xia JC, Weng DS, Li JT, et al. Loss of heterozygosity analysis of a candidate gastric carcinoma tumor suppressor locus at 7q31 [Ｊ]. Cancer Genet Cytogenet, 2006; 166(2)∶166.
15.	Chêne L, Giroud C, Desgrandchamps F, et al. Extensive analysis of the 7q31 region in human prostate tumors supports TES as the best candidate tumor suppressor gene [Ｊ]. Int J Cancer, 2004; 111(5)∶798.
16.	Forozan F, Veldman R, Ammerman CA, et al. Molecular cytogenetic analysis of 11 new breast cancer cell lines [Ｊ]. Br J Cancer, 1999; 81(8)∶1328.
17.	Hidalgo A, Monroy A, Arana RM, et al. Chromosomal imbalances in four new uterine cervix carcinoma derived cell lines [Ｊ]. BMC Cancer, 2003; 3(1)∶8.
18.	Wang Z, Shu W, Lu MM, et al. Wnt7b activates canonical signaling in epithelial and vascular smooth muscle cells through interactions with Fzd1, Fzd10, and LRP5 [Ｊ]. Mol Cell Biol, 2005; 25(12)∶5022.
19.	何渝軍，何雙梧，付濤. 大腸癌組織胃泌素與cmyc、cfos表達的關系 [Ｊ]. 中國普外基礎與臨床雜志, 2001; 8(1)∶15.
20.	文亞淵，劉寶華，洪勝龍，等. 凋亡相關基因survivin、caspase3及cyclinB1在胃癌發生、發展中的作用 [Ｊ]. 中國普外基礎與臨床雜志, 2006; 13(1)∶34.
21.	Duncan TJ, Watson NF, AlAttar AH, et al. The role of MUC1 and MUC3 in the biology and prognosis of colorectal cancer [Ｊ]. World J Surg Oncol, 2007; 9(5)∶31.
22.	Li Y, Asuri S, Rebhun JF, et al. The RAP1 guanine nucleotide exchange factor Epac2 couples cyclic AMP and Ras signals at the plasma membrane [Ｊ]. J Biol Chem, 2006; 281(5)∶2506.

1. 　彭志海, 凌云, 周崇治, 等. 散發性結直腸癌3號染色體等位基因雜合缺失 [J]. 中華醫學雜志, 2001; 81(6)∶336.
2. 　駱成玉，祝學光，李世擁, 等. nm23H1基因雜合性缺失及突變與大腸癌轉移抑制 [J]. 中國普外基礎與臨床雜志, 1999； 6(3)∶143.
3. 　Fornasarig M, Viel A, Bidoli E, et al. Amsterdam criteria Ⅱ and endometrial cancer index cases for an accurate selection of HNPCC families [J]. Tumori, 2002; 88(1)∶18.
4. 　Xiao XY, Zhou XY, Yan G, et al. Chromosomal alteration in Chinese sporadic colorectal carcinomas detected by comparative genomic hybridization [Ｊ]. Diagn Mol Pathol, 2007; 16(2)∶96.
5. 　van Dieren JM, Wink JC, Vissers KJ, et al. Chromosomal and microsatellite instability of adenocarcinomas and dysplastic lesions (DALM) in ulcerative colitis [J]. Diagn Mol Pathol, 2006; 15(4)∶216.
6. 　Pinto AE, Roque L, Rodrigues R, et al. Frequent 7q gains in flow cytometric multiploid/hypertetraploid breast carcinomas: a study of chromosome imbalances by comparative genomic hybridisation [Ｊ]. J Clin Pathol, 2006; 59(4)∶367.
7. 　Goodison S, Viars C, Urquidi V. Molecular cytogenetic analysis of a human breast metastasis model: identification of phenotypespecific chromosomal rearrangements [Ｊ]. Cancer Genet Cytogenet, 2005; 156(1)∶37.
8. 　Hasle H, Alonzo TA, Auvrignon A, et al. Monosomy 7 and deletion 7q in children and adolescents with acute myeloid leukemia: an international retrospective study [Ｊ]. Blood, 2007; 109(11)∶4641.
9. 　Smith AG, Worrillow LJ, Allan JM. A common genetic variant in XPD associates with risk of 5q and 7qdeleted acute myeloid leukemia [Ｊ]. Blood, 2007; 109(3)∶1233.
10. 　Trovato M, Ulivieri A, Dominici R, et al. Clinicopathological significance of celltypespecific loss of heterozygosity on chromosome 7q21: analysis of 318 microdissected thyroid lesions [Ｊ]. Endocr Relat Cancer, 2004; 11(2)∶365.
11. 　Trovato M, Fraggetta F, Villari D, et al. Loss of heterozygosity of the long arm of chromosome 7 in follicular and anaplastic thyroid cancer, but not in papillary thyroid cancer [Ｊ]. J Clin Endocrinol Metab, 1999; 84(9)∶3235.
12. 　Vanharanta S, Wortham NC, Langford C, et al. Definition of a minimal region of deletion of chromosome 7 in uterine leiomyomas by tilingpath microarray CGH and mutation analysis of known genes in this region [Ｊ]. Genes Chromosomes Cancer, 2007; 46(5)∶451.
13. 　Neville PJ, Thomas N, Campbell IG. Loss of heterozygosity at 7q22 and mutation analysis of the CDP gene in human epithelial ovarian tumors [Ｊ]. Int J Cancer, 2001; 91(3)∶345.
14. 　Xia JC, Weng DS, Li JT, et al. Loss of heterozygosity analysis of a candidate gastric carcinoma tumor suppressor locus at 7q31 [Ｊ]. Cancer Genet Cytogenet, 2006; 166(2)∶166.
15. 　Chêne L, Giroud C, Desgrandchamps F, et al. Extensive analysis of the 7q31 region in human prostate tumors supports TES as the best candidate tumor suppressor gene [Ｊ]. Int J Cancer, 2004; 111(5)∶798.
16. 　Forozan F, Veldman R, Ammerman CA, et al. Molecular cytogenetic analysis of 11 new breast cancer cell lines [Ｊ]. Br J Cancer, 1999; 81(8)∶1328.
17. 　Hidalgo A, Monroy A, Arana RM, et al. Chromosomal imbalances in four new uterine cervix carcinoma derived cell lines [Ｊ]. BMC Cancer, 2003; 3(1)∶8.
18. 　Wang Z, Shu W, Lu MM, et al. Wnt7b activates canonical signaling in epithelial and vascular smooth muscle cells through interactions with Fzd1, Fzd10, and LRP5 [Ｊ]. Mol Cell Biol, 2005; 25(12)∶5022.
19. 　何渝軍，何雙梧，付濤. 大腸癌組織胃泌素與cmyc、cfos表達的關系 [Ｊ]. 中國普外基礎與臨床雜志, 2001; 8(1)∶15.
20. 　文亞淵，劉寶華，洪勝龍，等. 凋亡相關基因survivin、caspase3及cyclinB1在胃癌發生、發展中的作用 [Ｊ]. 中國普外基礎與臨床雜志, 2006; 13(1)∶34.
21. 　Duncan TJ, Watson NF, AlAttar AH, et al. The role of MUC1 and MUC3 in the biology and prognosis of colorectal cancer [Ｊ]. World J Surg Oncol, 2007; 9(5)∶31.
22. 　Li Y, Asuri S, Rebhun JF, et al. The RAP1 guanine nucleotide exchange factor Epac2 couples cyclic AMP and Ras signals at the plasma membrane [Ｊ]. J Biol Chem, 2006; 281(5)∶2506.

《中國普外基礎與臨床雜志》

散發性結直腸癌染色體7q21－22區雜合性缺失精細定位

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《中國普外基礎與臨床雜志》

散發性結直腸癌染色體7q21－22區雜合性缺失精細定位

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