PAAF與ASPAAF誘導的腸損傷比較的實驗研究_《中國普外基礎與臨床雜志》

作者：

劉震 ¹ , 程若川 ² , 方向 ¹ , 張躍天 ¹ , 趙海劍 ² , 李俊 ¹ , 陳玉祥 ¹

1.四川省德陽市人民醫院普外科（德陽 618000）;;
2. 昆明醫學院第一附屬醫院普外科（昆明 650032）;

關鍵詞：

胰腺炎相關性腹水急性化膿性腹膜炎相關性腹水腸黏膜損傷腫瘤壞死因子-α

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目的　探討胰腺炎相關性腹水（PAAF）與急性化膿性腹膜炎相關性腹水（ASPAAF）誘導大鼠腸損傷的作用及其可能機理。
方法　以PAAF和ASPAAF分別誘導的SD大鼠腸損傷模型為研究對象，將48只SD大鼠隨機均分為3組。對照組腹腔內注射生理鹽水8 ml/只； PAAF組腹腔內注射PAAF 8 ml/只， ASPAAF組腹腔內注射ASPAAF 8 ml/只。分別于注射后6 h 和12 h分批處死大鼠，每時間點8只。測定血清內毒素和TNF-α水平，同時測定小腸組織中Na+-K+-ATP酶活性及TNF-α水平；于光鏡下觀察腸黏膜損傷情況并作等級比較。
結果　注射后6 h 和12 h， PAAF組和ASPAAF組血清內毒素和TNF-α水平, 小腸組織中TNF-α水平以及腸黏膜損傷程度均較對照組增高(P＜0.05),而Na+-K+-ATP酶活性則顯著降低（P＜0.05）； ASPAAF組血清內毒素和TNF-α水平,小腸組織中TNF-α水平以及腸黏膜損傷程度又均較PAAF組增高(P＜0.05),Na+-K+-ATP酶活性則顯著降低（P＜0.05）。
結論　PAAF及ASPAAF均能引起大鼠腸損傷，且ASPAAF引起的損傷更為嚴重。

引用本文： 劉震,程若川,方向,張躍天,趙海劍,李俊,陳玉祥. PAAF與ASPAAF誘導的腸損傷比較的實驗研究. 中國普外基礎與臨床雜志, 2008, 15(8): 555-559. doi: 復制

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16.	劉殿剛, 孫家邦, 李非, 等. 胰腺炎相關腹水對大鼠胃腸動力的影響 [J]. 北京醫學, 2005； 27（9）∶546.
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20.	van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers [J]. Blood, 1996； 87(12)∶ 5305.
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1. 　方向，程若川，趙海劍，等． PAAF 誘導腸損傷的實驗研究 [J]. 昆明醫學院學報， 2005； 26(2)∶ 47.
2. 　 Lankisch PG, Ihse I. Bile-induced acute experimental pancreatitis [J].Scand J Gastroenterol, 1987； 22(3)∶ 257.
3. 　 Chiu CJ, McArdle AH, Brown R, et al. Intestinal mucosal lesion in low-flow states. Ⅰ. A morphological, hemodynamic, and metabolic reappraisal [J]. Arch Surg， 1970； 101(4)∶ 478.
4. 　 Ogle CK, Mao JX, Wu JZ, et al. The 1994 Lindberg Award. The production of tumor necrosis factor, interleukin-1, interleukin-6, and prostaglandin E2 by isolated enterocytes and gut macrophages: effect of lipopolysaccharide and thermal injury[J]. Burn Care Rehabil， 1994； 15(6)∶ 470.
5. 　Erwin PJ, Lewis IF, Dolan S, et al. Lipopolysaccharide binding protein in acute pancreatitis [J]. Crit Care Med， 2000； 28(1)∶ 104.
6. 　 Makhija R, Kingsnorth AN. Cytockine storm in acute pancreatitis[J]. J Hepatobiliary Pancreat Surg， 2002； 9(4)∶ 401.
7. 　 Davies MG, Hagen PO. Systemic inflammatory response syndrome [J]. Br J Surg, 1997； 84(7)∶ 920.
8. 　 Gomez-Cambronero LG, Sabater L, Pereda J, et al. Role of cytokines and oxidative stress in the pathophysiology of acute pancreatitis: therapeutical implications [J]. Curr Drug Targets Inflamm Allergy, 2002; 1(4)∶ 393.
9. 　 Pastor CM, Frossard JL. Are genetically modified mice useful for the understanding of acute pancreatitis? [J]. FASEB J, 2001; 15(6)∶ 893.
10. 　 Leindler L, Morschl E, Laszlo F, et al. Importance of cytokines, nitric oxide, and apoptosis in the pathological process of necrotizing pancreatitis in rats [J]. Pancreas, 2004; 29(2)∶ 157.
11. 　Ding LA, Li JS. Gut in diseases: physiological elements and their clinical significance[J]. World J Gastroenterol, 2003; 9(11)∶ 2385.
12. 　Liu LR, Xia SH. Role of platelet-activating factor in the pathogenesis of acute pancreatitis[J]. World J Gastroenterol, 2006; 12(4)∶ 539.
13. 　Tracey KJ, Beutler B, Lowry SF, et al. Shock and tissue injury induced by recombinant human cachectin [J]. Science, 1986; 234(4775)∶ 470.
14. 　李紅光, 周總光, 雷松, 等. 人急性胰腺炎早期外周血單核細胞Toll樣受體4表達變化 [J]. 中國普外基礎與臨床雜志, 2006； 13（2）∶191.
15. 　陳哲宇，齊清會，嚴律南. 細菌性腹膜炎誘導大鼠多器官功能不全綜合征后結腸運動的變化和機理 [J]. 中國普外基礎與臨床雜志, 2006； 13（3）∶287.
16. 　劉殿剛, 孫家邦, 李非, 等. 胰腺炎相關腹水對大鼠胃腸動力的影響 [J]. 北京醫學, 2005； 27（9）∶546.
17. 　Choudhry MA, Rana SN, Kavanaugh MJ. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury[J]. Alcohol, 2004; 33(3)∶ 199.
18. 　De-Souza DA, Greene LJ. Intestinal permeability and systemic infections in critically ill patients: effect of glutamine [J]. Crit Care Med, 2005; 33(5)∶ 1125.
19. 　Vliagoftis H, Befus AD, Hollenberg MD, et al. Airway epithelial cells release eosinophil survival-promoting factors (GM-CSF) after stimulation of proteinase-activated receptor 2 [J]. J Allergy Clin Immunol, 2001； 107(4)∶ 679.
20. 　van Pelt LJ, Huisman MV, Weening RS, et al. A single dose of granulocyte-macrophage colony-stimulating factor induces systemic interleukin-8 release and neutrophil activation in healthy volunteers [J]. Blood, 1996； 87(12)∶ 5305.
21. 　Frossard JL, Saluja AK, Mach N , et al. In vivo evidence for the role of GM-CSF as a mediated in acute pancreatitis-associated lung injury [J]. Am J Physiol Lung Cell Mol Physiol, 2002； 283(3)∶ L541.
22. 　ONeill S, ONeill AJ, Conroy E, et al. Altered caspase expression results in delayed neutrophil apoptosis in acute pancreatitis [J]. J Leukoc Biol, 2000； 68(1)∶ 15.
23. 　Botha AJ, Moore FA, Moore EE. Early neutrophil sequestration alter injury: a pathogenic mechanism for multiple organ failure [J]. J Trauma， 1995； 39(3)∶ 411.

《中國普外基礎與臨床雜志》

PAAF與ASPAAF誘導的腸損傷比較的實驗研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

《中國普外基礎與臨床雜志》

PAAF與ASPAAF誘導的腸損傷比較的實驗研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料