肝素結合的類表皮生長因子促進大鼠減體積肝移植術后移植肝細胞的再生_《中國普外基礎與臨床雜志》

作者：

楊焱 , 李相成 , 王學浩 , 姚愛華 , 俞悅

南京醫科大學第一附屬醫院肝臟移植中心（南京210029）;

關鍵詞：

肝移植肝再生肝素結合的類表皮生長因子大鼠

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目的:探討肝素結合的類表皮生長因子(heparin binding epidermal growth factor-like growth factor, HB-EGF)對大鼠減體積肝移植術后肝細胞再生的促進作用。方法:采用改良“二袖套法”建立大鼠原位減體積肝移植模型,分為實驗組和對照組,術后即刻經尾靜脈分別給予HB-EGF(500 μg/kg)和相同體積的生理鹽水,2次/d。2組分別在術后第6 h、2 d、4 d及7 d隨機挑取5只大鼠處死,稱取移植物濕重,采血檢測血清丙氨酸轉氨酶(ALT)及白蛋白(Alb),流式細胞儀檢測移植肝細胞的增殖活性,免疫組織化學法檢測移植肝Ki-67的表達情況。結果:術后第6 h、2 d和4 d,實驗組移植物濕重較對照組相應時相明顯增加（P＜0.05）; 術后第6 h、2 d、4 d和7 d,實驗組血清ALT水平較對照組相應時相明顯降低(P＜0.05),而第4和7d時Alb水平較對照組相應時相明顯增高(P＜0.05);術后第2和4d時,實驗組的移植肝細胞增殖指數和Ki-67表達較對照組高（2d:P＜0.01; 4 d: P＜0.05。結論:大鼠原位減體積肝移植術后使用HB-EGF能夠明顯促進移植肝細胞的再生。

引用本文： 楊焱,李相成,王學浩,姚愛華,俞悅. 肝素結合的類表皮生長因子促進大鼠減體積肝移植術后移植肝細胞的再生. 中國普外基礎與臨床雜志, 2008, 15(3): 155-158. doi: 復制

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12.	Man K, Lo CM, Ng IO, et al. Liver transplantation in rats using smallforsize grafts: a study of hemodynamic and morphological changes [J]. Arch Surg, 2001; 136(3)∶280.
13.	Liang TB, Man K, KinWah Lee T, et al. Distinct intragraft response pattern in relation to graft size in liver transplantation [J]. Transplantation, 2003; 75(5)∶673.
14.	Man K, Lo CM, Lee TK, et al. Intragraft gene expression profiles by cDNA microarray in smallforsize liver grafts [J]. Liver Transpl, 2003; 9(4)∶425.
15.	Khai NC, Takahashi T, Ushikoshi H, et al. In vivo hepatic HBEGF gene transduction inhibits Fasinduced liver injury and induces liver regeneration in mice: a comparative study to HGF [J]. J Hepatol, 2006; 44(6)∶1046.
16.	Khandoga A, Biberthaler P, Enders G, et al. Pselectin mediates plateletendothelial cell interactions and reperfusion injury in the mouse liver in vivo [J]. Shock, 2002; 18(6)∶529.
17.	Rocourt DV, Mehta VB, Besner GE. Heparinbinding EGFlike growth factor decreases inflammatory cytokine expression after intestinal ischemia/reperfusion injury [J]. J Surg Res, 2007; 139(2)∶269.
18.	Shimizu H, Miyazaki M, Ito H, et al. Mechanism of cold ischemiareperfusioninduced graft injury after orthotopic liver transplantation in rats [J]. Hepatogastroenterology, 2001; 48(37)∶216.
19.	Wang J, Tahara K, Hakamata Y, et al. Auxiliary partial liver grafting in rats: effect of host hepatectomy on graft regeneration, and review of literature on surgical technique [J]. Microsurgery, 2002; 22(8)∶371.
20.	李相成, 王學浩, 俞悅, 等. 大鼠肝移植后肝再生的實驗研究 [J]. 中國普外基礎與臨床雜志, 2002； 9(4)∶228.

1. Nanba D, Kinugasa Y, Morimoto C, et al. Loss of HBEGF in smooth muscle or endothelial cell lineages causes heart malformation [J]. Biochem Biophys Res Commun, 2006; 350(2)∶315.
2. Shirakata Y, Kimura R, Nanba D, et al. Heparinbinding EGFlike growth factor accelerates keratinocyte migration and skin wound healing [J]. J Cell Sci, 2005; 118(Pt 11)∶2363.
3. Mine N, Iwamoto R, Mekada E. HBEGF promotes epithelial cell migration in eyelid development [J]. Development, 2005; 132(19)∶4317.
4. Xie H, Wang H, Tranguch S, et al. Maternal heparinbindingEGF deficiency limits pregnancy success in mice [J]. Proc Natl Acad Sci USA, 2007; 104(46)∶18315.
5. Clarke MC, Figg N, Maguire JJ, et al. Apoptosis of vascular smooth muscle cells induces features of plaque vulnerability in atherosclerosis [J]. Nat Med, 2006; 12(9)∶1075.
6. Ongusaha PP, Kwak JC, Zwible AJ, et al. HBEGF is a potent inducer of tumor growth and angiogenesis [J]. Cancer Res, 2004; 64(15)∶5283.
7. Kiso S, Kawata S, Tamura S, et al. Liver regeneration in heparinbinding EGFlike growth factor transgenic mice after partial hepatectomy [J]. Gastroenterology, 2003; 124(3)∶701.
8. 彭方興, 鄭本波, 劉立新, 等. 大鼠原位肝移植動物模型制作要點 [J]. 中國普外基礎與臨床雜志， 2005； 12（2）∶117.
9. Broelsch CE, Testa G, Alexandrou A, et al. Living related liver transplantation: medical and social aspects of a controversial therapy [J]. Gut, 2002; 50(2)∶143.
10. Broelsch CE, Burdelski M, Rogiers X, et al. Living donor for liver transplantation [J]. Hepatology, 1994; 20(1 Pt 2)∶49S.
11. Man K, Fan ST, Lo CM, et al. Graft injury in relation to graft size in right lobe live donor liver transplantation: a study of hepatic sinusoidal injury in correlation with portal hemodynamics and intragraft gene expression [J]. Ann Surg, 2003; 237(2)∶256.
12. Man K, Lo CM, Ng IO, et al. Liver transplantation in rats using smallforsize grafts: a study of hemodynamic and morphological changes [J]. Arch Surg, 2001; 136(3)∶280.
13. Liang TB, Man K, KinWah Lee T, et al. Distinct intragraft response pattern in relation to graft size in liver transplantation [J]. Transplantation, 2003; 75(5)∶673.
14. Man K, Lo CM, Lee TK, et al. Intragraft gene expression profiles by cDNA microarray in smallforsize liver grafts [J]. Liver Transpl, 2003; 9(4)∶425.
15. Khai NC, Takahashi T, Ushikoshi H, et al. In vivo hepatic HBEGF gene transduction inhibits Fasinduced liver injury and induces liver regeneration in mice: a comparative study to HGF [J]. J Hepatol, 2006; 44(6)∶1046.
16. Khandoga A, Biberthaler P, Enders G, et al. Pselectin mediates plateletendothelial cell interactions and reperfusion injury in the mouse liver in vivo [J]. Shock, 2002; 18(6)∶529.
17. Rocourt DV, Mehta VB, Besner GE. Heparinbinding EGFlike growth factor decreases inflammatory cytokine expression after intestinal ischemia/reperfusion injury [J]. J Surg Res, 2007; 139(2)∶269.
18. Shimizu H, Miyazaki M, Ito H, et al. Mechanism of cold ischemiareperfusioninduced graft injury after orthotopic liver transplantation in rats [J]. Hepatogastroenterology, 2001; 48(37)∶216.
19. Wang J, Tahara K, Hakamata Y, et al. Auxiliary partial liver grafting in rats: effect of host hepatectomy on graft regeneration, and review of literature on surgical technique [J]. Microsurgery, 2002; 22(8)∶371.
20. 李相成, 王學浩, 俞悅, 等. 大鼠肝移植后肝再生的實驗研究 [J]. 中國普外基礎與臨床雜志, 2002； 9(4)∶228.

《中國普外基礎與臨床雜志》

肝素結合的類表皮生長因子促進大鼠減體積肝移植術后移植肝細胞的再生

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《中國普外基礎與臨床雜志》

肝素結合的類表皮生長因子促進大鼠減體積肝移植術后移植肝細胞的再生

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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