半定量檢測膽管癌組織中人fxyd6基因的表達_《中國普外基礎與臨床雜志》

作者：

1.中國人民解放軍空軍總醫院（北京100036）;;
2.解放軍總醫院（北京100853）;;
3.軍事醫學科學院（北京100853）;

關鍵詞：

膽管癌人fxyd6基因離子轉運生物信息學聚合酶鏈反應

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目的研究人fxyd6基因在正常膽管與膽管癌組織中的表達,檢測該基因在膽管癌組織切片中的亞細胞表達定位.方法采用逆轉錄-聚合酶鏈反應(RT-PCR)方法檢測人fxyd6基因在正常膽管和膽管癌新鮮組織中的表達; 采用原位RT-PCR(IS-RT-PCR)方法原位檢測該基因在膽管癌石蠟組織切片中的亞細胞表達定位,并應用多媒體圖像分析軟件進行半定量分析.結果人fxyd6基因在正常膽管和膽管癌組織中存在差異性表達; 原位RT-PCR檢測表明人fxyd6基因定位于膽管癌上皮細胞胞漿內,在腫瘤細胞中的表達明顯高于在正常膽管細胞中的表達(P lt;0.05).結論人fxyd6基因在膽管癌的轉化、演進過程中可能起重要作用.

引用本文： 肖梅,周寧新,高麗杰,張愛群,郭維,高亞兵,彭瑞云,張效東,萬濤. 半定量檢測膽管癌組織中人fxyd6基因的表達. 中國普外基礎與臨床雜志, 2008, 15(2): 83-87. doi: 復制

1.	萬濤,倪彬,劉建偉,等.人膽管癌相關基因CCR-L2/FXYD6大片段克隆與鑒定[J].中華實驗外科雜志,2003; 20(5):393.
2.	Sweadner KJ,Rael E.The FXYD gene family of small ion transport regulators or channels:cDNA sequence,protein signature sequence,and expression[J].Genomics,2000; 68(1):41.
3.	申洪.免疫組織化學染色定量方法研究(Ⅲ)[J].中國組織化學與細胞化學雜志,1995; 4(1):90.
4.	Kowdley GC,Ackerman SJ,Chen Z,et al.Anion,cation,and zwitterion selectivity of phospholemman channel molecules[J].Biophys J,1997; 72(1):141.
5.	Minor NT,Sha Q,Nichols CG,et al.The gamma subunit of the Na,K-ATPase induces cation channel activity[J].Proc Natl Acad Sci USA,1998; 95(11):6521.
6.	Crambert G,Geering K.FXYD proteins:new tissue-specific regulators of the ubiquitous Na,K-ATPase[J].Sci STKE,2003; 2003(166):RE1.
7.	Garty H,Karlish SJ.Role of FXYD proteins in ion transport[J].Annu Rev Physiol,2006; 68:431.
8.	Clark HF,Gurney AL,Abaya E,et al.The secreted protein discovery initiative (SPDI),a large-scale effort to identify novel human secreted and transmembrane proteins:a bioinformatics assessment[J].Genome Res,2003; 13(10):2265.
9.	http://www.ncbi.nlm.nih.gov/Nucleotied/NCBI Nucleotide Data Web Page[DB].
10.	Rijken AM,Hu J,Perlman EJ,et al.Genomic alterations in distal bile duct carcinoma by comparative genomic hybridization and karyotype analysis[J].Genes Chromosomes Cancer,1999; 26(3):185.
11.	Mahlamki EH,Hglund M,Gorunova L,et al.Comparative genomic hybridization reveals frequent gains of 20q,8q,11q,12p,and 17q,and losses of 18q,9p,and 15q in pancreatic cancer[J].Genes Chromosomes Cancer,1997; 20(4):383.
12.	Gorunova L,Hglund M,Andrén-Sandberg A,et al.Cytogenetic analysis of pancreatic carcinomas:intratumor heterogeneity and nonrandom pattern of chromosome aberrations[J].Genes Chromosomes Cancer,1998; 23(2):81.
13.	Chen YJ,Chen PJ,Lee MC,et al.Chromosomal analysis of hepatic adenoma and focal nodular hyperplasia by comparative genomic hybridization[J].Genes Chromosomes Cancer,2002; 35(2):138.
14.	Pack SD,Karkera JD,Zhuang Z,et al.Molecular cytogenetic fingerprinting of esophageal squamous cell carcinoma by comparative genomic hybridization reveals a consistent pattern of chromosomal alterations[J].Genes Chromosomes Cancer,1999; 25(2):160.
15.	Chen YJ,Ko JY,Chen PJ,et al.Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization[J].Genes Chromosomes Cancer,1999; 25(2):169.

1. 萬濤,倪彬,劉建偉,等.人膽管癌相關基因CCR-L2/FXYD6大片段克隆與鑒定[J].中華實驗外科雜志,2003; 20(5):393.
2. Sweadner KJ,Rael E.The FXYD gene family of small ion transport regulators or channels:cDNA sequence,protein signature sequence,and expression[J].Genomics,2000; 68(1):41.
3. 申洪.免疫組織化學染色定量方法研究(Ⅲ)[J].中國組織化學與細胞化學雜志,1995; 4(1):90.
4. Kowdley GC,Ackerman SJ,Chen Z,et al.Anion,cation,and zwitterion selectivity of phospholemman channel molecules[J].Biophys J,1997; 72(1):141.
5. Minor NT,Sha Q,Nichols CG,et al.The gamma subunit of the Na,K-ATPase induces cation channel activity[J].Proc Natl Acad Sci USA,1998; 95(11):6521.
6. Crambert G,Geering K.FXYD proteins:new tissue-specific regulators of the ubiquitous Na,K-ATPase[J].Sci STKE,2003; 2003(166):RE1.
7. Garty H,Karlish SJ.Role of FXYD proteins in ion transport[J].Annu Rev Physiol,2006; 68:431.
8. Clark HF,Gurney AL,Abaya E,et al.The secreted protein discovery initiative (SPDI),a large-scale effort to identify novel human secreted and transmembrane proteins:a bioinformatics assessment[J].Genome Res,2003; 13(10):2265.
9. http://www.ncbi.nlm.nih.gov/Nucleotied/NCBI Nucleotide Data Web Page[DB].
10. Rijken AM,Hu J,Perlman EJ,et al.Genomic alterations in distal bile duct carcinoma by comparative genomic hybridization and karyotype analysis[J].Genes Chromosomes Cancer,1999; 26(3):185.
11. Mahlamki EH,Hglund M,Gorunova L,et al.Comparative genomic hybridization reveals frequent gains of 20q,8q,11q,12p,and 17q,and losses of 18q,9p,and 15q in pancreatic cancer[J].Genes Chromosomes Cancer,1997; 20(4):383.
12. Gorunova L,Hglund M,Andrén-Sandberg A,et al.Cytogenetic analysis of pancreatic carcinomas:intratumor heterogeneity and nonrandom pattern of chromosome aberrations[J].Genes Chromosomes Cancer,1998; 23(2):81.
13. Chen YJ,Chen PJ,Lee MC,et al.Chromosomal analysis of hepatic adenoma and focal nodular hyperplasia by comparative genomic hybridization[J].Genes Chromosomes Cancer,2002; 35(2):138.
14. Pack SD,Karkera JD,Zhuang Z,et al.Molecular cytogenetic fingerprinting of esophageal squamous cell carcinoma by comparative genomic hybridization reveals a consistent pattern of chromosomal alterations[J].Genes Chromosomes Cancer,1999; 25(2):160.
15. Chen YJ,Ko JY,Chen PJ,et al.Chromosomal aberrations in nasopharyngeal carcinoma analyzed by comparative genomic hybridization[J].Genes Chromosomes Cancer,1999; 25(2):169.

《中國普外基礎與臨床雜志》

半定量檢測膽管癌組織中人fxyd6基因的表達

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《中國普外基礎與臨床雜志》

半定量檢測膽管癌組織中人fxyd6基因的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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