胰腺癌細胞survivin表達RNAi抑制載體的構建及其抑制作用的研究_《中國普外基礎與臨床雜志》

作者：

何滿西 ¹ , 李耿 ² , 姚有貴 ² , 蒲道深 ² , 田伯樂 ¹ , 胡偉明 ¹ , 張肇達 ¹

1.四川大學華西醫院普外科(成都610041);;
2.四川省雅安市人民醫院肝膽外科(雅安625000);

關鍵詞：

RNA干擾胰腺癌生存蛋白凋亡

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的構建胰腺癌細胞生存蛋白(survivin)表達的RNA干擾(RNAi)抑制載體，并研究其對survivin表達的抑制作用。方法用免疫熒光技術和RT-PCR檢測胰腺癌細胞PANC-1中survivin及其mRNA的表達，并把survivin基因克隆到T載體進行測序；構建針對survivin缺失堿基基因和survivin基因的RNAi載體si-svv-1和si-svv-2，用脂質體轉染胰腺癌細胞PANC-1后，用RT-PCR、DNA梯狀電泳及流式細胞術分析比較2種干擾載體對survivin mRNA表達的抑制情況和檢測細胞凋亡。結果 survivin在胰腺癌細胞PANC-1中高表達； si-svv-2對survivin mRNA的表達抑制率達(72.43±8.04)%，而si-svv-1為0； si-svv-2能誘導胰腺癌細胞PANC-1的凋亡, 72 h細胞凋亡率達（12.36±1.44）%。結論本研究成功建立胰腺癌細胞survivin表達RNAi抑制載體，該載體可特異有效地抑制胰腺癌細胞PANC-1 survivin的表達并誘導胰腺癌細胞PANC-1凋亡。

引用本文： 何滿西,李耿,姚有貴,蒲道深,田伯樂,胡偉明,張肇達. 胰腺癌細胞survivin表達RNAi抑制載體的構建及其抑制作用的研究. 中國普外基礎與臨床雜志, 2008, 15(11): 829-834. doi: 復制

1.	Rubin P， Williams JP 主編．孫燕, 湯釗猷主譯. 臨床腫瘤學 [M]. 第8版．北京：人民衛生出版社， 2006∶768.
2.	陸星華．胰腺癌研究現狀與展望 [J]. 美中醫學， 2005； 2（3）∶73.
3.	MacKenzie MJ. Molecular therapy in pancreatic adenocarcinoma [J]. Lancet Oncol, 2004; 5(9)∶541.
4.	Lieberman J, Song E, Lee SK, et al. Interfering with disease: opportunities and roadblocks to harnessing RNA interference [J]. Trends Mol Med, 2003; 9(9)∶397.
5.	Olie RA, Simes-Wüst AP, Baumann B, et al. A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy [J]. Cancer Res, 2000; 60(11)∶2805.
6.	何滿西，孟令祥，唐勇，等． RNAi抑制胰腺癌細胞survivin表達并誘導細胞凋亡的實驗研究 [J]. 四川大學學報（醫學版）， 2006； 37（4）∶520.
7.	Tamm I, Wang Y, Sausville E, et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs [J]. Cancer Res, 1998; 58(23)∶5315.
8.	Islam A, Kageyama H, Takada N, et al. High expression of Survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma [J]. Oncogene, 2000; 19(5)∶617.
9.	李哲夫，陳孝平，張萬廣. 肝細胞癌合并門靜脈癌栓組織中PDGF和survivin表達的意義 [J]. 中國普外基礎與臨床雜志, 2007； 14（1）∶46.
10.	謝貽祥，孟剛. survivin在乳腺癌中的表達及其意義 [J]. 中國普外基礎與臨床雜志, 2007； 14（3）∶263.
11.	Wang Z, Xie Y, Wang H. Changes in survivin messenger RNA level during chemotherapy treatment in ovarian cancer cells[J].Cancer Biol Ther, 2005; 4(7)∶716.
12.	Van Valen F, Fulda S, Schfer KL, et al. Selective and nonselective toxicity of TRAIL/Apo2L combined with chemotherapy in human bone tumour cells vs. normal human cells [J]. Int J Cancer, 2003; 107(6)∶929.
13.	陳曦海，張豈凡，馬榮. 反義survivin誘導胃癌SGC7901細胞凋亡及逆轉耐藥的研究 [J].中國普外基礎與臨床雜志, 2007； 14（1）∶63.
14.	Satoh K, Kaneko K, Hirota M, et al. Expression of survivin is correlated with cancer cell apoptosis and is involved in the development of human pancreatic duct cell tumors [J]. Cancer, 2001; 92(2)∶271.
15.	Jinfeng M, Kimura W, Sakurai F,et al. Histopathological study of intraductal papillary mucinous tumor of the pancreas: special reference to the roles of Survivin and p53 in tumorigenesis of IPMT [J]. Int J Gastrointest Cancer, 2002; 32(2-3)∶73.
16.	王亞利，宋天保，王西京，等． Survivin mRNA反義寡核苷酸誘導胰腺癌細胞凋亡 [J]. 世界華人消化雜志， 2004； 12(8)∶1872.
17.	戴德堅, 陸才德, 賴日勇, 等．靶向抑制survivin對胰腺癌細胞增殖和凋亡效應的影響 [J]. 中華醫學雜志, 2004； 84（22）∶1894.
18.	Lu B, Mu Y, Cao C, et al. Survivin as a therapeutic target for radiation sensitization in lung cancer [J]. Cancer Res, 2004; 64(8)∶2840.
19.	Fulda S, Debatin KM. Sensitization for tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by the chemopreventive agent resveratrol [J]. Cancer Res, 2004; 64(1)∶337.
20.	唐朝暉, 鄒聲泉, 余彥, 等．不同劑量的X射線輻照對胰腺癌細胞Survivin表達的影響 [J]. 臨床外科雜志， 2007； 15（5）∶328.
21.	Williams NS, Gaynor RB, Scoggin S, et al. Identification and validation of genes involved in the pathogenesis of colorectal cancer using cDNA microarrays and RNA interference [J]. Clin Cancer Res, 2003; 9(3)∶931.
22.	Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J]. Nature, 1998; 391(6669)∶806.
23.	Miyagishi M, Taira K. Development and application of siRNA expression vector [J]. Nucleic Acids Res Suppl, 2002; 2(2)∶113.

1. Rubin P， Williams JP 主編．孫燕, 湯釗猷主譯. 臨床腫瘤學 [M]. 第8版．北京：人民衛生出版社， 2006∶768.
2. 陸星華．胰腺癌研究現狀與展望 [J]. 美中醫學， 2005； 2（3）∶73.
3. MacKenzie MJ. Molecular therapy in pancreatic adenocarcinoma [J]. Lancet Oncol, 2004; 5(9)∶541.
4. Lieberman J, Song E, Lee SK, et al. Interfering with disease: opportunities and roadblocks to harnessing RNA interference [J]. Trends Mol Med, 2003; 9(9)∶397.
5. Olie RA, Simes-Wüst AP, Baumann B, et al. A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy [J]. Cancer Res, 2000; 60(11)∶2805.
6. 何滿西，孟令祥，唐勇，等． RNAi抑制胰腺癌細胞survivin表達并誘導細胞凋亡的實驗研究 [J]. 四川大學學報（醫學版）， 2006； 37（4）∶520.
7. Tamm I, Wang Y, Sausville E, et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs [J]. Cancer Res, 1998; 58(23)∶5315.
8. Islam A, Kageyama H, Takada N, et al. High expression of Survivin, mapped to 17q25, is significantly associated with poor prognostic factors and promotes cell survival in human neuroblastoma [J]. Oncogene, 2000; 19(5)∶617.
9. 李哲夫，陳孝平，張萬廣. 肝細胞癌合并門靜脈癌栓組織中PDGF和survivin表達的意義 [J]. 中國普外基礎與臨床雜志, 2007； 14（1）∶46.
10. 謝貽祥，孟剛. survivin在乳腺癌中的表達及其意義 [J]. 中國普外基礎與臨床雜志, 2007； 14（3）∶263.
11. Wang Z, Xie Y, Wang H. Changes in survivin messenger RNA level during chemotherapy treatment in ovarian cancer cells[J].Cancer Biol Ther, 2005; 4(7)∶716.
12. Van Valen F, Fulda S, Schfer KL, et al. Selective and nonselective toxicity of TRAIL/Apo2L combined with chemotherapy in human bone tumour cells vs. normal human cells [J]. Int J Cancer, 2003; 107(6)∶929.
13. 陳曦海，張豈凡，馬榮. 反義survivin誘導胃癌SGC7901細胞凋亡及逆轉耐藥的研究 [J].中國普外基礎與臨床雜志, 2007； 14（1）∶63.
14. Satoh K, Kaneko K, Hirota M, et al. Expression of survivin is correlated with cancer cell apoptosis and is involved in the development of human pancreatic duct cell tumors [J]. Cancer, 2001; 92(2)∶271.
15. Jinfeng M, Kimura W, Sakurai F,et al. Histopathological study of intraductal papillary mucinous tumor of the pancreas: special reference to the roles of Survivin and p53 in tumorigenesis of IPMT [J]. Int J Gastrointest Cancer, 2002; 32(2-3)∶73.
16. 王亞利，宋天保，王西京，等． Survivin mRNA反義寡核苷酸誘導胰腺癌細胞凋亡 [J]. 世界華人消化雜志， 2004； 12(8)∶1872.
17. 戴德堅, 陸才德, 賴日勇, 等．靶向抑制survivin對胰腺癌細胞增殖和凋亡效應的影響 [J]. 中華醫學雜志, 2004； 84（22）∶1894.
18. Lu B, Mu Y, Cao C, et al. Survivin as a therapeutic target for radiation sensitization in lung cancer [J]. Cancer Res, 2004; 64(8)∶2840.
19. Fulda S, Debatin KM. Sensitization for tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by the chemopreventive agent resveratrol [J]. Cancer Res, 2004; 64(1)∶337.
20. 唐朝暉, 鄒聲泉, 余彥, 等．不同劑量的X射線輻照對胰腺癌細胞Survivin表達的影響 [J]. 臨床外科雜志， 2007； 15（5）∶328.
21. Williams NS, Gaynor RB, Scoggin S, et al. Identification and validation of genes involved in the pathogenesis of colorectal cancer using cDNA microarrays and RNA interference [J]. Clin Cancer Res, 2003; 9(3)∶931.
22. Fire A, Xu S, Montgomery MK, et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J]. Nature, 1998; 391(6669)∶806.
23. Miyagishi M, Taira K. Development and application of siRNA expression vector [J]. Nucleic Acids Res Suppl, 2002; 2(2)∶113.

《中國普外基礎與臨床雜志》

胰腺癌細胞survivin表達RNAi抑制載體的構建及其抑制作用的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

胰腺癌細胞survivin表達RNAi抑制載體的構建及其抑制作用的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料