2種體外腫瘤細胞藥敏檢測方法的比較研究_《中國普外基礎與臨床雜志》

作者：

周濤 , 胡亞 , 廖泉 , 趙玉沛

中國醫學科學院,中國協和醫科大學,北京協和醫院基本外科(北京100730);

關鍵詞：

膠原凝膠微滴腫瘤藥物敏感性檢測藥物敏感性測定腫瘤

視頻：

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目的通過WST-8和膠原凝膠微滴腫瘤藥物敏感性檢測(CD-DST) 2種藥敏檢測方法的比較，對CD-DST法進行評價。方法以胰腺癌細胞系SW1990、PCT-3和ASPC-1為對象，分別采用WST-8和CD-DST法測定其對5-氟尿嘧啶（5-FU）、健擇（GEM）和奧沙利鉑（OXA）3種藥物的敏感性。結果在一定的活細胞數目范圍內（500~10 000個），CD-DST法所測得的細胞積分光密度與細胞數目成線性正相關（r=0.991 1, P＜0.05）； 3種腫瘤細胞的生長抑制率CD-DST法均高于WST-8法(P＜0.05)，2種方法測定的5-FU、GEM和OXA藥物敏感性結果一致。結論 CD-DST法可用于胰腺癌的體外藥敏檢測，具有一定的臨床應用價值。

引用本文： 周濤,胡亞,廖泉,趙玉沛. 2種體外腫瘤細胞藥敏檢測方法的比較研究. 中國普外基礎與臨床雜志, 2008, 15(11): 825-828. doi: 復制

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3. Kobayashi H, Higashiyama M, Minamigawa K, et al. Examination of in vitro chemosensitivity test using collagen gel droplet culture method with colorimetric endpoint quantification [J]. Jpn J Cancer Res, 2001; 92(2)∶203.
4. Alley MC, Scudiero DA, Monks A, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay [J]. Cancer Res, 1988; 48(3)∶589.
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6. Richards J, Larson L, Yang J, et al. Method for culturing mammary epithelial cells in a rat tail collagen gel matrix [J]. Methods Cell Sci, 1983; 8(1)∶ 31.
7. Kawamura M, Gika M, Abiko T, et al. Clinical evaluation of chemosensitivity testing for patients with unresectable non-small cell lung cancer (NSCLC) using collagen gel droplet embedded culture drug sensitivity test (CD-DST) [J]. Cancer Chemother Pharmacol, 2007; 59(4)∶507.
8. Vaccarello L, Wang YL, Whiteside TL. Sustained outgrowth of autotumor-reactive T lymphocytes from human ovarian carcinomas in the presence of tumor necrosis factor alpha and interleukin 2 [J]. Hum Immunol, 1990; 28(2)∶216.
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10. Fricker SP, Buckley RG. Comparison of two colorimetric assays as cytotoxicity endpoints for an in vitro screen for antitumour agents [J]. Anticancer Res, 1996; 16(6B)∶3755.
11. Chen CF, Hwang JM, Wu CH, et al. Evaluation of a rapid tetrazolium-based colorimetric assay for selecting anticancer drugs [J]. Zhonghua Yi Xue Za Zhi (Taipei), 1990; 46(1)∶7.

《中國普外基礎與臨床雜志》

2種體外腫瘤細胞藥敏檢測方法的比較研究

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《中國普外基礎與臨床雜志》

2種體外腫瘤細胞藥敏檢測方法的比較研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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