iNOS及eNOS mRNA表達在急性壞死性胰腺炎肺損傷中的作用_《中國普外基礎與臨床雜志》

作者：

劉壘 , 王衛星 , 余佳 , 鄧文宏 , 陳辰 , 陳曉燕 , 崔周軍

武漢大學人民醫院普通外科(武漢 430060);

關鍵詞：

急性胰腺炎肺損傷誘導型一氧化氮合酶內皮型一氧化氮合酶基因表達

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目的通過觀察急性壞死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肺組織中誘導型一氧化氮合酶(inducible nitric oxide synthase,iNOS)mRNA和內皮型一氧化氮合酶(endothelial nitric oxide synthase,eNOS)mRNA的表達，探討內源性NOS在ANP肺損傷中的作用。
方法 40只Wistar大鼠隨機分為ANP組（n=30）和假手術組(SO組, n=10)，采用5%牛磺膽酸鈉逆行膽胰管注射法建立大鼠ANP模型。光鏡下觀察ANP造模后3 h、6 h及12 h時大鼠肺組織病理變化，并應用RT-PCR法檢測相應時相肺組織中iNOS及eNOS mRNA的表達水平。
結果隨著病程延長，ANP組肺組織可見不同程度的充血、水腫、炎性細胞浸潤、出血、壞死等病理損害，各時間點其肺損傷評分均明顯高于SO組(P lt;0.05)，且呈逐漸升高趨勢(P lt;0.05)。ANP組3 h、6 h及12 h iNOS和eNOS mRNA的表達水平較SO組均有明顯升高（P lt;0.05）。
結論肺組織中iNOS及eNOS mRNA的過度表達可能是大鼠ANP肺損傷發生的重要原因之一，這為采用抑制iNOS及eNOS mRNA的表達減輕ANP肺損傷的治療手段提供了理論依據。

引用本文： 劉壘,王衛星,余佳,鄧文宏,陳辰,陳曉燕,崔周軍. iNOS及eNOS mRNA表達在急性壞死性胰腺炎肺損傷中的作用. 中國普外基礎與臨床雜志, 2010, 17(5): 449-453. doi: 復制

1.	Lund H, Tnnesen H, Tnnesen MH, et al. Long-term recurrence and death rates after acute pancreatitis [J]. Scand J Gastroenterol, 2006; 41(2): 234-238.
2.	Ozturk M, Mas MR, Yasar M, et al. The role of inducible nitric oxide synthase inhibitor, meropenem, and taurine in experimental acute necrotizing pancreatitis [J]. Pancreas, 2003; 26(4): 357-362.
3.	von Dobschuetz E, Schmidt R, Scholtes M, et al. Protective role of heme oxygenase-1 in pancreatic microcirculatory dysfunction after ischemia/reperfusion in rats [J]. Pancreas, 2008; 36(4): 377-384.
4.	張瑩, 石承先, 別平, 等. 重癥急性胰腺炎大鼠肺臟iNOS mRNA表達及丹參的干預效應 [J]. 第三軍醫大學學報, 2005; 27(15): 1569-1572.
5.	Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease [J]. Physiol Rev, 2007; 87(1): 315-424.
6.	Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat [J]. Int J Pancreatol, 1992; 12(1): 41-51.
7.	Osman MO, Kristensen JU, Jacobsen NO, et al. A monoclonal anti-interleukin 8 antibody (WS-4) inhibits cytokine response and acute lung injury in experimental severe acute necrotising pancreatitis in rabbits [J]. Gut, 1998; 43(2): 232-239.
8.	Moncada S, Higgs A. The L-arginine-nitric oxide pathway [J]. N Engl J Med, 1993; 329(27): 2002-2012.
9.	Vassalli G, Dichek DA. Gene therapy for arterial thrombosis [J]. Cardiovasc Res, 1997; 35(3): 459-469.
10.	Werner J, Fernández-del Castillo C, Rivera JA, et al. On the protective mechanisms of nitric oxide in acute pancreatitis [J]. Gut, 1998; 43(3): 401-407.
11.	Molero X, Guarner F, Salas A, et al. Nitric oxide modulates pancreatic basal secretion and response to cerulein in the rat: effects in acute pancreatitis [J]. Gastroenterology, 1995; 108(6): 1855-1862.
12.	Hauser B, Matejovic M, Radermacher P. Nitric oxide, leukocytes and microvascular permeability: causality or bystanders? [J]. Crit Care, 2008; 12(1): 104.
13.	劉會芳, 趙燕燕, 崔玉英, 等. 川芎嗪對急性百草枯中毒大鼠一氧化氮和誘導型一氧化氮合酶的影響及其治療作用 [J]. 時珍國醫國藥, 2009; 20(7): 1631-1632.
14.	Liaudet L, Soriano FG, Szabó C. Biology of nitric oxide signaling [J]. Crit Care Med, 2000; 28(4 Suppl): N37-N52.
15.	Thomas DD, Ridnour LA, Isenberg JS, et al. The chemical biology of nitric oxide: implications in cellular signaling [J]. Free Radic Biol Med, 2008; 45(1): 18-31.
16.	Mohr S, Hallak H, de Boitte A, et al. Nitric oxide-induced S-glutathionylation and inactivation of glyceraldehyde-3-phosphate dehydrogenase [J]. J Biol Chem, 1999; 274(14): 9427-9430.
17.	孫雄, 龔鐳, 談春曉, 等. 急性膽源性胰腺炎患者血清NO、SOD、CAT 的變化及其臨床意義 [J]. 南京醫科大學學報(自然科學版), 2008; 28(10): 1290-1292.
18.	Camargo EA, Delbin MA, Ferreira T, et al. Influence of acute pancreatitis on the in vitro responsiveness of rat mesenteric and pulmonary arteries [J]. BMC Gastroenterol, 2008; 8: 19.
19.	張玉清, 喬建國, 譚最. 選擇性iNOS 抑制劑治療重癥急性胰腺炎的實驗研究 [J]. 陜西醫學雜志, 2003; 32(5): 473-475.
20.	Kutzsche S, Lyberg T, Bjertnaes LJ. et al. Effects of adenosine on extravascular lung water content in endotoxemic pigs [J]. Crit Care Med, 2001; 29(12): 2371-3276.
21.	Ang AD, Adhikari S, Ng SW, et al. Expression of nitric oxide synthase isoforms and nitric oxide production in acute pancreatitis and associated lung injury [J]. Pancreatology, 2009; 9(1-2): 150-159.
22.	Adhikari NK, Burns KE, Friedrich JO, et al. Effect of nitric oxide on oxygenation and mortality in acute lung injury: systematic review and meta-analysis [J]. BMJ, 2007; 334(7597): 779.
23.	張劍, 何生, 李茂德. 肝細胞癌中iNOS和p53蛋白的表達及其與腫瘤血管形成的關系 [J]. 中國普外基礎與臨床雜志, 2004; 11(5): 396-398.
24.	Ayub K, Serracino-Inglott F, Williamson RC, et al. Expression of inducible nitric oxide synthase contributes to the development of pancreatitis following pancreatic ischaemia and reperfusion [J]. Br J Surg, 2001; 88(9): 1189-1193.
25.	Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide [J]. Free Radic Biol Med, 1998; 25(4-5): 434-456.
26.	宋文哲, 宋燕, 譚巖, 等. 氨基胍對內毒素休克大鼠肝損傷的保護作用研究 [J]. 中國普外基礎與臨床雜志, 2003; 10(4): 347-350.
27.	Szabó C. Multiple pathways of peroxynitrite cytotoxicity [J]. Toxicol Lett, 2003; 140-141: 105-112.

1. Lund H, Tnnesen H, Tnnesen MH, et al. Long-term recurrence and death rates after acute pancreatitis [J]. Scand J Gastroenterol, 2006; 41(2): 234-238.
2. Ozturk M, Mas MR, Yasar M, et al. The role of inducible nitric oxide synthase inhibitor, meropenem, and taurine in experimental acute necrotizing pancreatitis [J]. Pancreas, 2003; 26(4): 357-362.
3. von Dobschuetz E, Schmidt R, Scholtes M, et al. Protective role of heme oxygenase-1 in pancreatic microcirculatory dysfunction after ischemia/reperfusion in rats [J]. Pancreas, 2008; 36(4): 377-384.
4. 張瑩, 石承先, 別平, 等. 重癥急性胰腺炎大鼠肺臟iNOS mRNA表達及丹參的干預效應 [J]. 第三軍醫大學學報, 2005; 27(15): 1569-1572.
5. Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease [J]. Physiol Rev, 2007; 87(1): 315-424.
6. Schmidt J, Lewandrowsi K, Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat [J]. Int J Pancreatol, 1992; 12(1): 41-51.
7. Osman MO, Kristensen JU, Jacobsen NO, et al. A monoclonal anti-interleukin 8 antibody (WS-4) inhibits cytokine response and acute lung injury in experimental severe acute necrotising pancreatitis in rabbits [J]. Gut, 1998; 43(2): 232-239.
8. Moncada S, Higgs A. The L-arginine-nitric oxide pathway [J]. N Engl J Med, 1993; 329(27): 2002-2012.
9. Vassalli G, Dichek DA. Gene therapy for arterial thrombosis [J]. Cardiovasc Res, 1997; 35(3): 459-469.
10. Werner J, Fernández-del Castillo C, Rivera JA, et al. On the protective mechanisms of nitric oxide in acute pancreatitis [J]. Gut, 1998; 43(3): 401-407.
11. Molero X, Guarner F, Salas A, et al. Nitric oxide modulates pancreatic basal secretion and response to cerulein in the rat: effects in acute pancreatitis [J]. Gastroenterology, 1995; 108(6): 1855-1862.
12. Hauser B, Matejovic M, Radermacher P. Nitric oxide, leukocytes and microvascular permeability: causality or bystanders? [J]. Crit Care, 2008; 12(1): 104.
13. 劉會芳, 趙燕燕, 崔玉英, 等. 川芎嗪對急性百草枯中毒大鼠一氧化氮和誘導型一氧化氮合酶的影響及其治療作用 [J]. 時珍國醫國藥, 2009; 20(7): 1631-1632.
14. Liaudet L, Soriano FG, Szabó C. Biology of nitric oxide signaling [J]. Crit Care Med, 2000; 28(4 Suppl): N37-N52.
15. Thomas DD, Ridnour LA, Isenberg JS, et al. The chemical biology of nitric oxide: implications in cellular signaling [J]. Free Radic Biol Med, 2008; 45(1): 18-31.
16. Mohr S, Hallak H, de Boitte A, et al. Nitric oxide-induced S-glutathionylation and inactivation of glyceraldehyde-3-phosphate dehydrogenase [J]. J Biol Chem, 1999; 274(14): 9427-9430.
17. 孫雄, 龔鐳, 談春曉, 等. 急性膽源性胰腺炎患者血清NO、SOD、CAT 的變化及其臨床意義 [J]. 南京醫科大學學報(自然科學版), 2008; 28(10): 1290-1292.
18. Camargo EA, Delbin MA, Ferreira T, et al. Influence of acute pancreatitis on the in vitro responsiveness of rat mesenteric and pulmonary arteries [J]. BMC Gastroenterol, 2008; 8: 19.
19. 張玉清, 喬建國, 譚最. 選擇性iNOS 抑制劑治療重癥急性胰腺炎的實驗研究 [J]. 陜西醫學雜志, 2003; 32(5): 473-475.
20. Kutzsche S, Lyberg T, Bjertnaes LJ. et al. Effects of adenosine on extravascular lung water content in endotoxemic pigs [J]. Crit Care Med, 2001; 29(12): 2371-3276.
21. Ang AD, Adhikari S, Ng SW, et al. Expression of nitric oxide synthase isoforms and nitric oxide production in acute pancreatitis and associated lung injury [J]. Pancreatology, 2009; 9(1-2): 150-159.
22. Adhikari NK, Burns KE, Friedrich JO, et al. Effect of nitric oxide on oxygenation and mortality in acute lung injury: systematic review and meta-analysis [J]. BMJ, 2007; 334(7597): 779.
23. 張劍, 何生, 李茂德. 肝細胞癌中iNOS和p53蛋白的表達及其與腫瘤血管形成的關系 [J]. 中國普外基礎與臨床雜志, 2004; 11(5): 396-398.
24. Ayub K, Serracino-Inglott F, Williamson RC, et al. Expression of inducible nitric oxide synthase contributes to the development of pancreatitis following pancreatic ischaemia and reperfusion [J]. Br J Surg, 2001; 88(9): 1189-1193.
25. Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide [J]. Free Radic Biol Med, 1998; 25(4-5): 434-456.
26. 宋文哲, 宋燕, 譚巖, 等. 氨基胍對內毒素休克大鼠肝損傷的保護作用研究 [J]. 中國普外基礎與臨床雜志, 2003; 10(4): 347-350.
27. Szabó C. Multiple pathways of peroxynitrite cytotoxicity [J]. Toxicol Lett, 2003; 140-141: 105-112.

《中國普外基礎與臨床雜志》

iNOS及eNOS mRNA表達在急性壞死性胰腺炎肺損傷中的作用

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《中國普外基礎與臨床雜志》

iNOS及eNOS mRNA表達在急性壞死性胰腺炎肺損傷中的作用

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