103鈀放射性支架誘導人膽管癌細胞凋亡及對Fas基因表達的影響_《中國普外基礎與臨床雜志》

作者：

趙曉丹 ¹ , 何貴金 ²

1.東港市中心醫院普外科(遼寧東港 118300);;
2.中國醫科大學盛京醫院肝膽乳腺外科(沈陽 110004);

關鍵詞：

人膽管癌細胞 103Pd放射性支架 Fas基因細胞凋亡 TUNEL法免疫組織化學法

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目的探討103鈀(Pd)放射性支架釋放的γ射線對Fas基因表達的影響以及與膽管癌細胞凋亡的關系及意義
方法建立人膽管癌裸鼠移植瘤模型；將建模成功的裸鼠分為實驗組和對照組，實驗組置入37 MBq 103Pd膽道放射性支架，對照組置入普通金屬支架；支架置入后10 d計算腫瘤體積; 應用TUNEL法檢測膽管癌細胞凋亡的情況; 免疫組化染色方法檢測膽管癌細胞中Fas基因表達的情況。
結果實驗組腫瘤體積較對照組增長明顯緩慢(P lt;0.05); 膽管癌細胞的Fas基因表達陽性率較對照組明顯增高(P lt;0.05)，且膽管癌細胞出現明顯凋亡，細胞凋亡率明顯高于對照組(P lt;0.01)。
結論 103Pd放射性支架可誘導人膽管癌裸鼠移植瘤中膽管癌細胞凋亡，明顯抑制膽管癌細胞生長，其可能通過增強Fas基因表達促進膽管癌細胞凋亡，可能是103Pd放射性支架治療膽管癌的重要機理之一，進一步為臨床應用103Pd放射性支架治療膽管癌提供了理論依據。

引用本文： 趙曉丹,何貴金. 103鈀放射性支架誘導人膽管癌細胞凋亡及對Fas基因表達的影響. 中國普外基礎與臨床雜志, 2010, 17(3): 224-228. doi: 復制

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12.	Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin2activated cytotoxic lymphocytes combined with radiation and anticancer drugs [J]. Eur J Cancer, 2000; 36(15): 20072017.
13.	Mundle SD, Raza A. Defining the dynamics of selfassembled Fasreceptor activation [J]. Trends Immunol， 2002; 23(4): 187194.
14.	Hengartner MO. The biochemistry of apoptosis [J]. Nature, 2000; 407(6805): 770776.
15.	Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy [J]. Exp Cell Res, 2000; 256(1): 4249.
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1. Itamochi H, Yamasaki F, Sudo T, et al. Reduction of radiationinduced apoptosis by specific expression of Bcl2 in normal cells [J]. Cancer Gene Ther, 2006; 13(5): 451459.
2. Peschel RE, Colberg JW, Chen Z, et al. Iodine 125 versus palladium 103 implants for prostate cancer: clinical outcomes and complications [J]. Cancer J, 2004; 10(3): 170174.
3. Waksman R, Raizner AE, Yeung AC, et al. Use of localised intracoronary beta radiation in treatment of in stent restenosis: the INHIBIT randomised controlled trial [J]. Lancet, 2002; 359(9306): 551557.
4. 何貴金, 于發強, 高沁怡, 等. 103鈀放射性支架對Fas基因表達影響及與膽管癌細胞凋亡關系研究 [J]. 中國普外基礎與臨床雜志, 2007; 14(3): 284286.
5. He GJ, Sun DD, Ji DW, et al. Induction of biliary cholangiocarcinoma cell apoptosis by 103Pd cholangial radioactive stent γrays [J]. Chin Med J (Engl), 2008; 121(11): 10201024.
6. Patel T. Cholangiocarcinoma [J]. Nat Clin Pract Gastroenterol Hepatol, 2006; 3(1): 3342.
7. Nakeeb A, Pitt HA, Sohn TA, et al. Cholangiocarcinoma. A spectrum of intrahepatic, perihilar, and distal tumors [J]. Ann Surg, 1996; 224(4): 463473.
8. Ahrendt SA, Pitt HA. Biliary tract [A]. In: Townsend. Sabiston Textbook of Surgery [M]. 16th ed. Philadelphia: WB Saunders Company, 2003: 10761111.
9. Catelas I, Petit A, Zukor DJ, et al. Cytotoxic and apoptotic effects of cobalt and chromium ions on J774 macrophages-Implication of caspase3 in the apoptotic pathway [J]. J Mater Sci Mater Med, 2001; 12(1012): 949953.
10. Uno M, Otsuki T, Yata K, et al. Participation of Fasmediated apoptotic pathway in KB, a human head and neck squamous cell carcinoma cell line, after irradiation [J]. Int J Oncol, 2002; 20(3): 617622.
11. Chakraborty M, Abrams SI, Coleman CN, et al. External beam radiation of tumors alters phenotype of tumor cells to render them susceptible to vaccinemediated Tcell killing [J]. Cancer Res, 2004; 64(12): 43284337.
12. Yamamoto T, Yoneda K, Ueta E, et al. Enhanced apoptosis of squamous cell carcinoma cells by interleukin2activated cytotoxic lymphocytes combined with radiation and anticancer drugs [J]. Eur J Cancer, 2000; 36(15): 20072017.
13. Mundle SD, Raza A. Defining the dynamics of selfassembled Fasreceptor activation [J]. Trends Immunol， 2002; 23(4): 187194.
14. Hengartner MO. The biochemistry of apoptosis [J]. Nature, 2000; 407(6805): 770776.
15. Kaufmann SH, Earnshaw WC. Induction of apoptosis by cancer chemotherapy [J]. Exp Cell Res, 2000; 256(1): 4249.
16. Khosravi R, Maya R, Gottlieb T, et al. Rapid ATMdependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage [J]. Proc Natl Acad Sci USA, 1999; 96(26): 1497314977.
17. Schuler M, Green DR. Mechanisms of p53dependent apoptosis [J]. Biochem Soc Trans, 2001; 29(Pt 6): 684688.
18. Sheard MA. Ionizing radiation as a responseenhancing agent for CD95mediated apoptosis [J]. Int J Cancer, 2001; 96(4): 213220.
19. Bratton SB, MacFarlane M, Cain K, et al. Protein complexes activate distinct caspase cascades in death receptor and stressinduced apoptosis [J]. Exp Cell Res, 2000; 256(1): 2733.

《中國普外基礎與臨床雜志》

103鈀放射性支架誘導人膽管癌細胞凋亡及對Fas基因表達的影響

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《中國普外基礎與臨床雜志》

103鈀放射性支架誘導人膽管癌細胞凋亡及對Fas基因表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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