腺苷2A受體激活抑制大鼠T淋巴細胞功能_《中國普外基礎與臨床雜志》

作者：

朱劍飛 ¹ , 湯黎明 ¹ , 王鳳 ² , 陸華虎 ² , 任俊 ¹

1.南京醫科大學附屬常州第二人民醫院普外科（江蘇常州 213003）;;
2.南京醫科大學附屬第一醫院肝臟中心(南京 210029);

關鍵詞：

腺苷受體 T淋巴細胞活化 T淋巴細胞增殖細胞毒效應

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的研究激活腺苷2A受體對大鼠T淋巴細胞體外活化、增殖及細胞毒效應的影響。方法植物血凝素（phytohemagglutinin，PHA）刺激小鼠脾臟來源的T淋巴細胞，并以終濃度為0.01 μmol/L、0.1 μmol/L、1 μmol/L及10 μmol/L的腺苷2A受體激動劑CGS21680與其共培養。熒光抗體染色結合流式細胞術檢測T淋巴細胞活化抗原CD69及CD25的表達及細胞增殖情況，ELISA法檢測T淋巴細胞分泌IL-2及INF-γ的水平。結果各濃度的CGS21680均可明顯抑制T淋巴細胞表面CD25及CD69分子的表達（P lt;0.05，P lt;0.01），抑制T淋巴細胞增殖（P lt;0.01），也對IL-2及INF-γ的分泌產生明顯抑制作用（P lt;0.01）。結論激活腺苷2A受體能有效地抑制小鼠T淋巴細胞的體外活化、增殖及細胞毒作用。

引用本文： 朱劍飛,湯黎明,王鳳,陸華虎,任俊. 腺苷2A受體激活抑制大鼠T淋巴細胞功能. 中國普外基礎與臨床雜志, 2010, 17(11): 1136-1140. doi: 復制

1.	Zarek PE, Huang CT, Lutz ER, et al. A2A receptor signaling promotes peripheral tolerance by inducing T-cell anergy and the generation of adaptive regulatory T cells [J]. Blood, 2008; 111(1): 251-259.
2.	Tang LM, Wang YP, Wang K, et al. Protective effect of adenosine A2A receptor activation in small-for-size liver transplantation [J]. Transpl Int, 2007; 20(1): 93-101.
3.	Tan EY, Richard CL, Zhang H, et al. Adenosine downregulates DPPIV on HT-29 colon cancer cells by stimulating protein tyrosine phosphatase(s) and reducing ERK1/2 activity via a novel pathway [J]. Am J Physiol Cell Physiol, 2006; 291(3): C433-C444.
4.	Liang X, Yu JJ, Reed E. Protein phosphatase 2A interacts with Chk2 and regulates its phosphorylation at Thr-68 after cisplatin treatment of human ovarian cancer cells [A]. AACR Meeting Abstracts. Anaheim, CA, 2005: 1309.
5.	Liang X, Reed E, Yu JJ. Caffeine enhances cisplatin-induced phosphorylation of Chk 2 at Thr-68 via inhibition of adenosine receptor signaling in ovarian cancer cells [A]. AACR Meeting Abstracts. Orlando, Florida, 2004: 605.
6.	Lau CL, Zhao Y, Kron IL, et al. The role of adenosine A2A receptor signaling in bronchiolitis obliterans [J]. Ann Thorac Surg, 2009; 88(4): 1071-1078.
7.	Csóka B, Németh ZH, Virág L, et al. A2A adenosine receptors and C/EBPbeta are crucially required for IL-10 production by macrophages exposed to Escherichia coli [J]. Blood, 2007; 110(7): 2685-2695.
8.	Luijk B, van den Berge M, Kerstjens HA, et al. Effect of an inhaled adenosine A2A agonist on the allergen-induced late asthmatic response [J]. Allergy, 2008; 63(1): 75-80.
9.	Grenz A, Baier D, Petroktistis F, et al. Theophylline improves early allograft function in rat kidney transplantation [J]. J Pharmacol Exp Ther, 2006; 317(2): 473-479.
10.	Reece TB, Ellman PI, Maxey TS, et al. Adenosine A2A receptor activation reduces inflammation and preserves pulmonary function in an in vivo model of lung transplantation [J]. J Thorac Cardiovasc Surg, 2005; 129(5): 1137-1143.
11.	Varani K, Laghi-Pasini F, Camurri A, et al. Changes of peripheral A2A adenosine receptors in chronic heart failure and cardiac transplantation [J]. FASEB J, 2003; 17(2): 280-282.
12.	Lappas CM, Liu PC, Linden J, et al. Adenosine A2A receptor activation limits graft-versus-host disease after allogenic hematopoietic stem cell transplantation [J]. J Leukoc Biol, 2010; 87(2): 345-354.
13.	Schmitz ML, Bacher S, Dienz O. NF-kappaB activation pathways induced by T cell costimulation [J]. FASEB J, 2003; 17(15): 2187-2193.
14.	Hughes K, Edin S, Antonsson A, et al. Calmodulin-dependent kinase Ⅱ mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase [J]. J Biol Chem, 2001; 276(38): 36008-36013.
15.	Pule MA, Straathof KC, Dotti G, et al. Three-module signaling endo-domain artifical T-cell receptor which transmits CD28, OX40 and CD3-zeta signals enhances IL-2 release and proliferative response in transduced primary T-cells [J]. Blood (ASH Annual Meeting Abstracts), 2004; 104(11): 1747.
16.	Srinivasan S, Bolick DT, Lukashev D, et al. Sphingosine-1-phosphate reduces CD4+ T-cell activation in type 1 diabetes through regulation of hypoxia-inducible factor short isoform I.1 and CD69 [J]. Diabetes, 2008; 57(2): 484-493.
17.	Hermann-Kleiter N, Thuille N, Pfeifhofer C, et al. PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes [J]. Blood, 2006; 107(12): 4841-4848.
18.	Nakamura K, Kitani A, Fuss I, et al. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice [J]. J Immunol, 2004; 172(2): 834-842.
19.	Oppenheim JJ. IL-2: more than a T cell growth factor [J]. J Immunol, 2007; 179(3): 1413-1414.
20.	Santoli D, Clark SC, Kreider BL, et al. Amplification of IL-2-driven T cell proliferation by recombinant human IL-3 and granulocyte-macrophage colony-stimulating factor [J]. J Immunol, 1988; 141(2): 519-526.
21.	Carrizosa E, Gomez TS, Labno CM, et al. Hematopoietic lineage cell-specific protein 1 is recruited to the immunological synapse by IL-2-inducible T cell kinase and regulates phospholipase Cgamma1 Microcluster dynamics during T cell spreading [J]. J Immunol, 2009; 183(11): 7352-7761.
22.	Erdmann AA, Gao ZG, Jung U, et al. Activation of Th1 and Tc1 cell adenosine A2A receptors directly inhibits IL-2 secretion in vitro and IL-2-driven expansion in vivo [J]. Blood, 2005; 105(12): 4707-4714.
23.	Yamamoto S, Yamamoto T, Kataoka T, et al. Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN 〔correction of INF〕 and augment IFN-mediated 〔correction of INF〕 natural killer activity [J]. J Immunol, 1992; 148(12): 4072-4076.
24.	Gallin M, Edmonds K, Ellner JJ, et al. Cell-mediated immune responses in human infection with Onchocerca volvulus [J]. J Immunol, 1988; 140(6): 1999-2007.
25.	Niessner A, Sato K, Chaikof EL, et al. Pathogen-sensing plasmacytoid dendritic cells stimulate cytotoxic T-cell function in the atherosclerotic plaque through interferon-alpha [J]. Circulation, 2006; 114(23): 2482-2489.

1. Zarek PE, Huang CT, Lutz ER, et al. A2A receptor signaling promotes peripheral tolerance by inducing T-cell anergy and the generation of adaptive regulatory T cells [J]. Blood, 2008; 111(1): 251-259.
2. Tang LM, Wang YP, Wang K, et al. Protective effect of adenosine A2A receptor activation in small-for-size liver transplantation [J]. Transpl Int, 2007; 20(1): 93-101.
3. Tan EY, Richard CL, Zhang H, et al. Adenosine downregulates DPPIV on HT-29 colon cancer cells by stimulating protein tyrosine phosphatase(s) and reducing ERK1/2 activity via a novel pathway [J]. Am J Physiol Cell Physiol, 2006; 291(3): C433-C444.
4. Liang X, Yu JJ, Reed E. Protein phosphatase 2A interacts with Chk2 and regulates its phosphorylation at Thr-68 after cisplatin treatment of human ovarian cancer cells [A]. AACR Meeting Abstracts. Anaheim, CA, 2005: 1309.
5. Liang X, Reed E, Yu JJ. Caffeine enhances cisplatin-induced phosphorylation of Chk 2 at Thr-68 via inhibition of adenosine receptor signaling in ovarian cancer cells [A]. AACR Meeting Abstracts. Orlando, Florida, 2004: 605.
6. Lau CL, Zhao Y, Kron IL, et al. The role of adenosine A2A receptor signaling in bronchiolitis obliterans [J]. Ann Thorac Surg, 2009; 88(4): 1071-1078.
7. Csóka B, Németh ZH, Virág L, et al. A2A adenosine receptors and C/EBPbeta are crucially required for IL-10 production by macrophages exposed to Escherichia coli [J]. Blood, 2007; 110(7): 2685-2695.
8. Luijk B, van den Berge M, Kerstjens HA, et al. Effect of an inhaled adenosine A2A agonist on the allergen-induced late asthmatic response [J]. Allergy, 2008; 63(1): 75-80.
9. Grenz A, Baier D, Petroktistis F, et al. Theophylline improves early allograft function in rat kidney transplantation [J]. J Pharmacol Exp Ther, 2006; 317(2): 473-479.
10. Reece TB, Ellman PI, Maxey TS, et al. Adenosine A2A receptor activation reduces inflammation and preserves pulmonary function in an in vivo model of lung transplantation [J]. J Thorac Cardiovasc Surg, 2005; 129(5): 1137-1143.
11. Varani K, Laghi-Pasini F, Camurri A, et al. Changes of peripheral A2A adenosine receptors in chronic heart failure and cardiac transplantation [J]. FASEB J, 2003; 17(2): 280-282.
12. Lappas CM, Liu PC, Linden J, et al. Adenosine A2A receptor activation limits graft-versus-host disease after allogenic hematopoietic stem cell transplantation [J]. J Leukoc Biol, 2010; 87(2): 345-354.
13. Schmitz ML, Bacher S, Dienz O. NF-kappaB activation pathways induced by T cell costimulation [J]. FASEB J, 2003; 17(15): 2187-2193.
14. Hughes K, Edin S, Antonsson A, et al. Calmodulin-dependent kinase Ⅱ mediates T cell receptor/CD3- and phorbol ester-induced activation of IkappaB kinase [J]. J Biol Chem, 2001; 276(38): 36008-36013.
15. Pule MA, Straathof KC, Dotti G, et al. Three-module signaling endo-domain artifical T-cell receptor which transmits CD28, OX40 and CD3-zeta signals enhances IL-2 release and proliferative response in transduced primary T-cells [J]. Blood (ASH Annual Meeting Abstracts), 2004; 104(11): 1747.
16. Srinivasan S, Bolick DT, Lukashev D, et al. Sphingosine-1-phosphate reduces CD4+ T-cell activation in type 1 diabetes through regulation of hypoxia-inducible factor short isoform I.1 and CD69 [J]. Diabetes, 2008; 57(2): 484-493.
17. Hermann-Kleiter N, Thuille N, Pfeifhofer C, et al. PKCtheta and PKA are antagonistic partners in the NF-AT transactivation pathway of primary mouse CD3+ T lymphocytes [J]. Blood, 2006; 107(12): 4841-4848.
18. Nakamura K, Kitani A, Fuss I, et al. TGF-beta 1 plays an important role in the mechanism of CD4+CD25+ regulatory T cell activity in both humans and mice [J]. J Immunol, 2004; 172(2): 834-842.
19. Oppenheim JJ. IL-2: more than a T cell growth factor [J]. J Immunol, 2007; 179(3): 1413-1414.
20. Santoli D, Clark SC, Kreider BL, et al. Amplification of IL-2-driven T cell proliferation by recombinant human IL-3 and granulocyte-macrophage colony-stimulating factor [J]. J Immunol, 1988; 141(2): 519-526.
21. Carrizosa E, Gomez TS, Labno CM, et al. Hematopoietic lineage cell-specific protein 1 is recruited to the immunological synapse by IL-2-inducible T cell kinase and regulates phospholipase Cgamma1 Microcluster dynamics during T cell spreading [J]. J Immunol, 2009; 183(11): 7352-7761.
22. Erdmann AA, Gao ZG, Jung U, et al. Activation of Th1 and Tc1 cell adenosine A2A receptors directly inhibits IL-2 secretion in vitro and IL-2-driven expansion in vivo [J]. Blood, 2005; 105(12): 4707-4714.
23. Yamamoto S, Yamamoto T, Kataoka T, et al. Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN 〔correction of INF〕 and augment IFN-mediated 〔correction of INF〕 natural killer activity [J]. J Immunol, 1992; 148(12): 4072-4076.
24. Gallin M, Edmonds K, Ellner JJ, et al. Cell-mediated immune responses in human infection with Onchocerca volvulus [J]. J Immunol, 1988; 140(6): 1999-2007.
25. Niessner A, Sato K, Chaikof EL, et al. Pathogen-sensing plasmacytoid dendritic cells stimulate cytotoxic T-cell function in the atherosclerotic plaque through interferon-alpha [J]. Circulation, 2006; 114(23): 2482-2489.

《中國普外基礎與臨床雜志》

腺苷2A受體激活抑制大鼠T淋巴細胞功能

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

腺苷2A受體激活抑制大鼠T淋巴細胞功能

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料