重癥急性胰腺炎大鼠肝臟毛細血管滲漏的發生機理_《中國普外基礎與臨床雜志》

作者：

李洪森 , 黃鶴光

福建醫科大學附屬協和醫院普外科(福建福州 350001);

關鍵詞：

重癥急性胰腺炎腫瘤壞死因子-α occludin 毛細血管滲漏

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討重癥急性胰腺炎(SAP)大鼠肝臟毛細血管滲漏的發生機理。方法40只SD大鼠采用完全隨機法隨機分為假手術(sham operation，SO)組和SAP組，SAP按不同的取材時間又分為3、6、12及24 h 4個亞組，每組8只。以胰膽管逆行注射5%牛磺膽酸鈉建立SAP模型，觀察各組肝臟組織病理改變，檢測血清腫瘤壞死因子(TNF)-α水平，干濕重法檢測肝臟組織含水率，免疫組織化學方法檢測肝臟組織中occludin蛋白表達水平，RTPCR法檢測肝臟組織中occludin mRNA表達水平。結果SO組肝臟組織病理無明顯改變; SAP組肝臟組織表現為典型SAP病理改變，包括血管擴張明顯、組織疏松水腫、炎性細胞浸潤。SAP組各時相TNF-α水平、肝臟組織含水率逐漸升高，在觀察時間范圍內，24 h時均達最高，TNF-α水平、肝臟組織含水率在SAP組不同時相比較差異均有統計學意義(P lt;0.05)，且SAP組不同時相均明顯高于SO組(P lt;0.05)。SAP 組3 h時occludin蛋白和mRNA水平均開始降低，6 h時達最低值，12 h和24 h時較6 h時明顯升高(P lt;0.05)，但仍均低于SO組(P lt;0.05)。結論SAP大鼠肝臟發生嚴重的毛細血管滲漏，可能與SAP時TNF-α表達上調，導致緊密連接蛋白occludin及其mRNA表達下調有關。

引用本文： 李洪森,黃鶴光. 重癥急性胰腺炎大鼠肝臟毛細血管滲漏的發生機理. 中國普外基礎與臨床雜志, 2011, 18(6): 599-602. doi: 復制

1.	楊波, 黃鶴光, 陳大良, 等. 逆行性胰膽管注射法制作重癥急性胰腺炎大鼠模型 [J]. 福建醫科大學學報, 2002, 36(1): 7172.
2.	陳德招, 黃鶴光. 羥乙基淀粉(130/0.4)對急性壞死性胰腺炎大鼠腦毛細血管滲漏的影響 [J]. 中華胰腺病雜志, 2010, 10(3): 213214.
3.	Kingsnorth A. Role of cytokines and their inhibitors in acute pancreatitis [J]. Gut, 1997, 40(1): 14.
4.	Karne S, Gorelick FS. Etiopathogenesis of acute pancreatitis [J]. Surg Clin North Am, 1999, 79(4): 699710．.
5.	藤澤林. 非手術治療重癥急性胰腺炎23例報告 [J]. 新醫學, 2001, 32(5): 285286．.
6.	Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [J]. J Surg Res, 2000, 90(2): 95101．.
7.	Chui DW, Grendell JH. Acute pancreatitis [J]. Curr Opin Gastroenterol, 1993, 9(6): 760766.
8.	Fink MP, Delude RL. Epithelial barrier dysfunction: a unifying theme to explain the pathogenesis of multiple organ dysfunction at the cellular level [J]. Crit Care Clin, 2005, 21(2): 177196.
9.	Fallon MB, Brecher AR, Balda MS, et al. Altered hepatic localization and expression of occludin after common bile duct ligation [J]. Am J Physiol, 1995, 269(4 Pt 1): C1057C1062.
10.	Malminen M, Koivukangas V, Peltonen J, et al. Immunohistological distribution of the tight junction components ZO1 and occludin in regenerating human epidermis [J]. Br J Dermatol, 2003, 149(2): 255260.
11.	Kevil CG, Okayama N, Trocha SD, et al. Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: role of occludin in endothelial solute barriers [J]. Microcirculation, 1998, 5(23): 197210.
12.	Hirase T, Staddon JM, Saitou M, et al. Occludin as a possible determinant of tight junction permeability in endothelial cells [J]. J Cell Sci, 1997, 110(Pt 14): 16031613.
13.	Morcos Y, Hosie MJ, Bauer HC, et al. Immunolocalization of occludin and claudin1 to tight junctions in intact CNS vessels of mammalian retina [J]. J Neurocytol, 2001, 30(2): 107123.
14.	Panek J, Zasada J, Po＇zniczek M. Microcirculatory disturbance in the course of acute pancreatitis [J]. Przegl Lek, 2007, 64(6): 435437.

1. 楊波, 黃鶴光, 陳大良, 等. 逆行性胰膽管注射法制作重癥急性胰腺炎大鼠模型 [J]. 福建醫科大學學報, 2002, 36(1): 7172.
2. 陳德招, 黃鶴光. 羥乙基淀粉(130/0.4)對急性壞死性胰腺炎大鼠腦毛細血管滲漏的影響 [J]. 中華胰腺病雜志, 2010, 10(3): 213214.
3. Kingsnorth A. Role of cytokines and their inhibitors in acute pancreatitis [J]. Gut, 1997, 40(1): 14.
4. Karne S, Gorelick FS. Etiopathogenesis of acute pancreatitis [J]. Surg Clin North Am, 1999, 79(4): 699710．.
5. 藤澤林. 非手術治療重癥急性胰腺炎23例報告 [J]. 新醫學, 2001, 32(5): 285286．.
6. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [J]. J Surg Res, 2000, 90(2): 95101．.
7. Chui DW, Grendell JH. Acute pancreatitis [J]. Curr Opin Gastroenterol, 1993, 9(6): 760766.
8. Fink MP, Delude RL. Epithelial barrier dysfunction: a unifying theme to explain the pathogenesis of multiple organ dysfunction at the cellular level [J]. Crit Care Clin, 2005, 21(2): 177196.
9. Fallon MB, Brecher AR, Balda MS, et al. Altered hepatic localization and expression of occludin after common bile duct ligation [J]. Am J Physiol, 1995, 269(4 Pt 1): C1057C1062.
10. Malminen M, Koivukangas V, Peltonen J, et al. Immunohistological distribution of the tight junction components ZO1 and occludin in regenerating human epidermis [J]. Br J Dermatol, 2003, 149(2): 255260.
11. Kevil CG, Okayama N, Trocha SD, et al. Expression of zonula occludens and adherens junctional proteins in human venous and arterial endothelial cells: role of occludin in endothelial solute barriers [J]. Microcirculation, 1998, 5(23): 197210.
12. Hirase T, Staddon JM, Saitou M, et al. Occludin as a possible determinant of tight junction permeability in endothelial cells [J]. J Cell Sci, 1997, 110(Pt 14): 16031613.
13. Morcos Y, Hosie MJ, Bauer HC, et al. Immunolocalization of occludin and claudin1 to tight junctions in intact CNS vessels of mammalian retina [J]. J Neurocytol, 2001, 30(2): 107123.
14. Panek J, Zasada J, Po＇zniczek M. Microcirculatory disturbance in the course of acute pancreatitis [J]. Przegl Lek, 2007, 64(6): 435437.

《中國普外基礎與臨床雜志》

重癥急性胰腺炎大鼠肝臟毛細血管滲漏的發生機理

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

重癥急性胰腺炎大鼠肝臟毛細血管滲漏的發生機理

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料