嵌合錨定T淋巴細胞對肝癌細胞增殖的阻滯效應_《中國普外基礎與臨床雜志》

作者：

徐宏勇 ¹ , 徐立 ² , 李開宗 ³

1.解放軍第451醫院肝膽普外科(陜西西安 710054);;
2.第四軍醫大學西京醫院消化病研究所(陜西西安 710032);;
3.第四軍醫大學西京醫院肝膽外科(陜西西安 710032);

關鍵詞：

肝癌腫瘤相關糖蛋白72抗原單鏈抗體片段 T淋巴細胞

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目的探討腫瘤相關糖蛋白72(TAG72)靶向性嵌合錨定T淋巴細胞的制備方法，并檢測它對TAG72陽性肝癌細胞增殖的阻滯效應。方法分離外周血單核細胞(PBMC)，然后用免疫磁珠法分離得到CD8+ T淋巴細胞。將重組真核表達載體anti-TAG72-scFv-CD28-pcDNA3.0采用脂質體介導的細胞轉染和細胞培養，以制備TAG72靶向性的嵌合錨定T淋巴細胞; 將嵌合錨定T淋巴細胞與TAG72陽性肝癌細胞SMMC7721共培養，通過流式細胞儀檢測肝癌細胞的周期變化，分析嵌合錨定T淋巴細胞對肝癌細胞增殖的抑制效應。結果TAG72靶向性嵌合錨定T淋巴細胞可識別肝癌細胞SMMC7721；用流式細胞儀檢測發現，嵌合錨定T淋巴細胞可引起肝癌細胞SMMC7721的增殖阻滯。結論TAG72靶向性嵌合錨定T淋巴細胞可特異性識別TAG72陽性肝癌細胞SMMC7721并引起其增殖阻滯。

引用本文： 徐宏勇,徐立,李開宗. 嵌合錨定T淋巴細胞對肝癌細胞增殖的阻滯效應. 中國普外基礎與臨床雜志, 2011, 18(4): 370-373. doi: 復制

1.	徐宏勇, 徐立, 李開宗, 等. 抗TAG72抗原單鏈抗體的原核表達及對人肝癌的檢測 [J]. 中國普外基礎與臨床雜志, 2010, 17(7): 699702.
2.	Ponnusamy MP, Venkatraman G, Singh AP, et al. Expression of TAG72 in ovarian cancer and its correlation with tumor stage and patient prognosis [J]. Cancer Lett，2007, 251(2): 247257.
3.	Chauhan SC, Vinayek N, Maher DM, et al. Combined staining of TAG72, MUC1, and CA125 improves labeling sensitivity in ovarian cancer: antigens for multitargeted antibodyguided therapy [J]. J Histochem Cytochem, 2007, 55(8): 867875.
4.	June CH, Bluestone JA, Nadler LM, et al. The B7 and CD28 receptor families [J]. Immunol Today, 1994, 15(7): 321331.
5.	Petersen CC, Petersen MS, Agger R, et al. Accumulation in tumor tissue of adoptively transferred T cells: A comparison between intravenous and intraperitoneal injection [J]. J Immunother, 2006, 29(3): 241249.
6.	徐宏勇, 徐立, 李開宗, 等. 靶向性腫瘤相關抗原TAG72的scFvCD28嵌合基因的真核表達載體的構建 [J].中國普外基礎與臨床雜志, 2002, 9(6): 398401.
7.	徐宏勇, 徐立, 高建宏, 等. 嵌合錨定T細胞致癌胚抗原陽性胃癌細胞的凋亡效應 [J]. 中華醫學雜志, 2007, 87(15): 10531057.
8.	Willemsen RA, Weijtens ME, Ronteltap C, et al. Grafting primary human T lymphocytes with cancerspecific chimeric single chain and two chain TCR [J]. Gene Ther, 2000, 7(16): 13691377.
9.	Ychou M, Duffour J, Kramar A, et al. Clinical significance and prognostic value of CA724 compared with TAG72 and CA199 in patients with gastric cancer [J]. Dis Markers, 2000, 16(34): 105110.
10.	Hombach A, Heuser C, Marquardt T, et al. CD4+ T cells engrafted with a recombination immunorecrpter efficiently lyse target cells in a MHC antigen and Fas independent fashion [J]. J Immuno, 2001, 167(2): 10901095.
11.	Hombach A, Wieczarkowiecz A, Marquardt T, et al. Tumorspecific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient IL2 secretion and can be integrated into one combined CD28/CD3 zeta signaling receptor molecule [J]. J Immunol, 2001, 167(11): 61236130.
12.	Lee EY, Kim C, Kim MJ, et al. Signet ring cell carcinoma of the extrahepatic bile duct [J]. Gut Liver, 2010, 4(3): 402406.
13.	Gupta S, Sathishkumar S, Ahmed MM. influence of cell cycle checkpoints and p53 function on the toxicity of temozolomide in human pancreatic cancer cells [J]. Pancreatology, 2010, 10(5): 565579.
14.	Spitzner M, Emons G, Kramer F, et al. A gene expression signature for chemoradiosensitivity of colorectal cancer cells [J]. Int J Radiat Oncol Biol Phys, 2010, 78(4): 11841192.
15.	WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest and apoptosis induced by oxaliplatin (LOHP) on four human cancer cell lines [J]. Anticancer Res, 2006, 26(3A): 20932099.
16.	WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest by oxaliplatin on cancer cells [J]. Fundam Clin Pharmacol, 2007, 21(2): 165172.

1. 徐宏勇, 徐立, 李開宗, 等. 抗TAG72抗原單鏈抗體的原核表達及對人肝癌的檢測 [J]. 中國普外基礎與臨床雜志, 2010, 17(7): 699702.
2. Ponnusamy MP, Venkatraman G, Singh AP, et al. Expression of TAG72 in ovarian cancer and its correlation with tumor stage and patient prognosis [J]. Cancer Lett，2007, 251(2): 247257.
3. Chauhan SC, Vinayek N, Maher DM, et al. Combined staining of TAG72, MUC1, and CA125 improves labeling sensitivity in ovarian cancer: antigens for multitargeted antibodyguided therapy [J]. J Histochem Cytochem, 2007, 55(8): 867875.
4. June CH, Bluestone JA, Nadler LM, et al. The B7 and CD28 receptor families [J]. Immunol Today, 1994, 15(7): 321331.
5. Petersen CC, Petersen MS, Agger R, et al. Accumulation in tumor tissue of adoptively transferred T cells: A comparison between intravenous and intraperitoneal injection [J]. J Immunother, 2006, 29(3): 241249.
6. 徐宏勇, 徐立, 李開宗, 等. 靶向性腫瘤相關抗原TAG72的scFvCD28嵌合基因的真核表達載體的構建 [J].中國普外基礎與臨床雜志, 2002, 9(6): 398401.
7. 徐宏勇, 徐立, 高建宏, 等. 嵌合錨定T細胞致癌胚抗原陽性胃癌細胞的凋亡效應 [J]. 中華醫學雜志, 2007, 87(15): 10531057.
8. Willemsen RA, Weijtens ME, Ronteltap C, et al. Grafting primary human T lymphocytes with cancerspecific chimeric single chain and two chain TCR [J]. Gene Ther, 2000, 7(16): 13691377.
9. Ychou M, Duffour J, Kramar A, et al. Clinical significance and prognostic value of CA724 compared with TAG72 and CA199 in patients with gastric cancer [J]. Dis Markers, 2000, 16(34): 105110.
10. Hombach A, Heuser C, Marquardt T, et al. CD4+ T cells engrafted with a recombination immunorecrpter efficiently lyse target cells in a MHC antigen and Fas independent fashion [J]. J Immuno, 2001, 167(2): 10901095.
11. Hombach A, Wieczarkowiecz A, Marquardt T, et al. Tumorspecific T cell activation by recombinant immunoreceptors: CD3 zeta signaling and CD28 costimulation are simultaneously required for efficient IL2 secretion and can be integrated into one combined CD28/CD3 zeta signaling receptor molecule [J]. J Immunol, 2001, 167(11): 61236130.
12. Lee EY, Kim C, Kim MJ, et al. Signet ring cell carcinoma of the extrahepatic bile duct [J]. Gut Liver, 2010, 4(3): 402406.
13. Gupta S, Sathishkumar S, Ahmed MM. influence of cell cycle checkpoints and p53 function on the toxicity of temozolomide in human pancreatic cancer cells [J]. Pancreatology, 2010, 10(5): 565579.
14. Spitzner M, Emons G, Kramer F, et al. A gene expression signature for chemoradiosensitivity of colorectal cancer cells [J]. Int J Radiat Oncol Biol Phys, 2010, 78(4): 11841192.
15. WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest and apoptosis induced by oxaliplatin (LOHP) on four human cancer cell lines [J]. Anticancer Res, 2006, 26(3A): 20932099.
16. WilliamFaltaos S, Rouillard D, Lechat P, et al. Cell cycle arrest by oxaliplatin on cancer cells [J]. Fundam Clin Pharmacol, 2007, 21(2): 165172.

《中國普外基礎與臨床雜志》

嵌合錨定T淋巴細胞對肝癌細胞增殖的阻滯效應

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《中國普外基礎與臨床雜志》

嵌合錨定T淋巴細胞對肝癌細胞增殖的阻滯效應

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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