ORP150在重癥急性胰腺炎大鼠胰腺中表達變化的研究_《中國普外基礎與臨床雜志》

作者：

熊星鋮 , 陳辰 , 王衛星 , 鄧文宏 , 李金友 , 余佳 , 孫海濤

武漢大學人民醫院普通外科（湖北武漢 430060）;

關鍵詞：

重癥急性胰腺炎氧調節蛋白150 胰腺損傷

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目的探討氧調節蛋白150（ORP150）在重癥急性胰腺炎（SAP）大鼠胰腺損傷中的表達變化。方法將40只雄性Wistar大鼠隨機分為：假手術組（SO組，10只）、SAP 組（30只，其中SAP 3、6及12 h 3個時相各10只）。 SO組開腹后僅翻動胰腺則關腹， SAP組則經膽胰管逆行注射5%牛磺膽酸鈉制備SAP模型；測定SO組術后12 h和SAP組造模后3、6及12 h的腹水量、血清淀粉酶（AMY）及谷氨酸氨基轉移酶（ALT）水平；于光鏡下進行胰腺組織病理學評分；用RT-PCR 法檢測胰腺組織中ORP150 mRNA的表達。結果SAP組各時相腹水量，AMY及ALT 水平和胰腺病理評分均明顯高于SO 組（P lt;0.05）。 SAP組腹水量在造模后3 h 和6 h 之間差異無統計學意義（P gt;0.05），而12 h 較前2個時相均明顯增高（P lt;0.05）。 SAP組的AMY及ALT 水平和胰腺組織病理學評分在造模后各時相兩兩間比較差異均有統計學意義（P lt;0.05）。 ORP150 mRNA 在SO組呈低表達，在SAP組造模后各時相的表達強度均較SO組增強，其中造模后 3 h 表達強度最高，6 h和12 h依次降低。結論ORP150 mRNA 在SAP 胰腺組織中呈高表達，提示ORP150 可能在SAP 胰腺損傷的發生、發展過程中起著一定的作用。

引用本文： 熊星鋮,陳辰,王衛星,鄧文宏,李金友,余佳,孫海濤. ORP150在重癥急性胰腺炎大鼠胰腺中表達變化的研究. 中國普外基礎與臨床雜志, 2011, 18(11): 1165-1168. doi: 復制

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6.	Kobayashi T, Ogawa S, Yura T, et al. Abundant expression of 150kDa oxygenregulated protein in mouse pancreatic beta cells is correlated with insulin secretion [J]. Biochem Biophys Rese Commun, 2000， 267(3): 831837.
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10.	Eibl G, Buhr HJ, Foitzik T. Therapy of mircrocirculatory disorders in severe acute pancreatitis: what mediators should we block? [J]. Intensive Care Med， 2002， 28(2): 139146.
11.	Kaneda S, Yura T, Yanagi H. Production of three distinct mRNAs of 150 kDa oxygenregulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions [J]. J Biochem, 2000, 128(3): 529538.
12.	Kitao Y, Hashimoto K, Matsuyama T, et al. ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development [J]. J Neurosci, 2004, 24(6): 14861496.
13.	Aleshin AN, Sawa Y, KitagawaSakakida S, et al. 150kDa oxygenregulated protein attenuates myocardial ischemiareperfusion injury in rat hear [J]. J Mol Cell Cardiol, 2005, 38(3): 517525.
14.	Wang Y, Wu Z, Li D, et al. Involvement of oxygenregulated protein 150 in AMPactivated protein kinasemediated alleviation of lipidinduced endoplasmic reticulum stress [J]. J Biol Chem., 2011, 286(13): 1111911131.
15.	Telek G, Ducroc R, Scoazec JY, et al. Differential upregulation of cellular adhesion molecules at the sites of oxidative stress in experimental acute pancreatitis [J]. J Surg Res, 2001, 96(1): 5667.
16.	Seo JY, Kim H, Seo JT, et al. Oxidative stress induced cytokine production in isolated rat pancreatic acinar cells: effects of smallmolecule antioxidants [J]. Pharmacology, 2002, 64(2): 6370.
17.	Chen H, Zheng C, Zhang X, et al. Apelin alleviates diabetesassociated endoplasmic reticulum stress in the pancreas of Akita mice [J]. Peptides, 2011, 32(8): 16341639.
18.	Modak MA, Parab PB, Ghaskadbi SS. Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets [J]. Islets, 2011, 3(5): 234240.
19.	Deng WH, Chen C, Wang WX, et al. Effects of ORP150 on appearance and function of pancreatic beta cells following acute necrotizing pancreatitis [J]. Pathol Res Pract, 2011, 207(6): 370376.
20.	Kitao Y, Matsuyama T, Takano K, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)induced neurotoxicity? [J]. Antioxid Redox Signal, 2007, 9(5): 589595.
21.	Arrington DD, Schnellmann RG. Targeting of the molecular chaperone oxygenregulated protein 150 (ORP150) to mitochondria and its induction by cellular stress [J]. Am J Physiol Cell Physiol, 2008, 294(2): C641C650.
22.	Paszkowski AS, Rau B, Mayer JM, et al. Therapeutic application of caspase 1/interleukin1betaconverting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis [J]. Ann Surg, 2002, 235(1): 6876.
23.	Chen C, Xu S, Wang WX, et al. Rosiglitazone attenuates the severity of sodium taurocholateinduced acute pancreatitis and pancreatitisassociated lung injury [J]. Arch Med Res, 2009, 40(2): 7988.
24.	Hoque R, Sohail M, Malik A, et al. TLR9 and the NLRP3 inflammasome link acinar cell death with inflammation in acute pancreatitis [J]. Gastroenterology, 2011, 141(1): 358369.

1. 皮執民. 消化外科學 [M]. 北京: 人民衛生出版社, 2002: 5558.
2. 張圣道, 雷若慶. 重癥胰腺炎治療的爭論、進展和發展趨勢 [J]. 中國實用外科雜志, 2002， 22(1): 2223.
3. 林旭紅, 李永渝. 急性胰腺炎發病機制及相關治療的研究進展 [J]. 中國病理生理雜志, 2010， 26(5): 10291032, 1040.
4. Schmidt J, Rattner DW, Lewandrowski K, et al. A better model of acute pancreatitis for evaluating therapy [J]. Ann Surg, 1992, 215(1): 4456.
5. Kobayashi T, Ohta Y. Enforced expression of oxygenregulated protein, ORP150, induces vacuolar degeneration in mouse myocardium [J]. Transgenic Res, 2003， 12(1): 1322.
6. Kobayashi T, Ogawa S, Yura T, et al. Abundant expression of 150kDa oxygenregulated protein in mouse pancreatic beta cells is correlated with insulin secretion [J]. Biochem Biophys Rese Commun, 2000， 267(3): 831837.
7. Kobayashi T, Ohta Y. 150kDa oxygenregulated protein is an essential factor for insulin release [J]. Pancreas， 2005， 30(4): 299306.
8. Werner J, Hartwig W, Uhl W, et al. Useful markers for predicting severity and monitoring progression of acute pancreatitis [J]. Pancreatology， 2003， 3(2): 115127.
9. Dobosz M, Mionskowska L, Hac S, et al. Heparin improves organ microcirculatory disturbances in caeruleininduced acute pancreatitis in rats [J]. World J Castroenterol， 2004， 10(17): 25532556.
10. Eibl G, Buhr HJ, Foitzik T. Therapy of mircrocirculatory disorders in severe acute pancreatitis: what mediators should we block? [J]. Intensive Care Med， 2002， 28(2): 139146.
11. Kaneda S, Yura T, Yanagi H. Production of three distinct mRNAs of 150 kDa oxygenregulated protein (ORP150) by alternative promoters: preferential induction of one species under stress conditions [J]. J Biochem, 2000, 128(3): 529538.
12. Kitao Y, Hashimoto K, Matsuyama T, et al. ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development [J]. J Neurosci, 2004, 24(6): 14861496.
13. Aleshin AN, Sawa Y, KitagawaSakakida S, et al. 150kDa oxygenregulated protein attenuates myocardial ischemiareperfusion injury in rat hear [J]. J Mol Cell Cardiol, 2005, 38(3): 517525.
14. Wang Y, Wu Z, Li D, et al. Involvement of oxygenregulated protein 150 in AMPactivated protein kinasemediated alleviation of lipidinduced endoplasmic reticulum stress [J]. J Biol Chem., 2011, 286(13): 1111911131.
15. Telek G, Ducroc R, Scoazec JY, et al. Differential upregulation of cellular adhesion molecules at the sites of oxidative stress in experimental acute pancreatitis [J]. J Surg Res, 2001, 96(1): 5667.
16. Seo JY, Kim H, Seo JT, et al. Oxidative stress induced cytokine production in isolated rat pancreatic acinar cells: effects of smallmolecule antioxidants [J]. Pharmacology, 2002, 64(2): 6370.
17. Chen H, Zheng C, Zhang X, et al. Apelin alleviates diabetesassociated endoplasmic reticulum stress in the pancreas of Akita mice [J]. Peptides, 2011, 32(8): 16341639.
18. Modak MA, Parab PB, Ghaskadbi SS. Control of hyperglycemia significantly improves oxidative stress profile of pancreatic islets [J]. Islets, 2011, 3(5): 234240.
19. Deng WH, Chen C, Wang WX, et al. Effects of ORP150 on appearance and function of pancreatic beta cells following acute necrotizing pancreatitis [J]. Pathol Res Pract, 2011, 207(6): 370376.
20. Kitao Y, Matsuyama T, Takano K, et al. Does ORP150/HSP12A protect dopaminergic neurons against MPTP/MPP(+)induced neurotoxicity? [J]. Antioxid Redox Signal, 2007, 9(5): 589595.
21. Arrington DD, Schnellmann RG. Targeting of the molecular chaperone oxygenregulated protein 150 (ORP150) to mitochondria and its induction by cellular stress [J]. Am J Physiol Cell Physiol, 2008, 294(2): C641C650.
22. Paszkowski AS, Rau B, Mayer JM, et al. Therapeutic application of caspase 1/interleukin1betaconverting enzyme inhibitor decreases the death rate in severe acute experimental pancreatitis [J]. Ann Surg, 2002, 235(1): 6876.
23. Chen C, Xu S, Wang WX, et al. Rosiglitazone attenuates the severity of sodium taurocholateinduced acute pancreatitis and pancreatitisassociated lung injury [J]. Arch Med Res, 2009, 40(2): 7988.
24. Hoque R, Sohail M, Malik A, et al. TLR9 and the NLRP3 inflammasome link acinar cell death with inflammation in acute pancreatitis [J]. Gastroenterology, 2011, 141(1): 358369.

《中國普外基礎與臨床雜志》

ORP150在重癥急性胰腺炎大鼠胰腺中表達變化的研究

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《中國普外基礎與臨床雜志》

ORP150在重癥急性胰腺炎大鼠胰腺中表達變化的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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