目的:探討丙型肝炎病毒非結構蛋白NS4B在原發性肝癌發生中的作用,及其發生機制。方法:設置對照組、空白載體PCXN2組、轉染NS4B組。使用脂質體介導法,轉染丙型肝炎病毒非結構蛋白重組質粒NS4B進入Chang肝細胞內,并用G418篩選。繪制生長曲線,分別用流式細胞儀檢測瞬時表達及穩定表達時肝細胞凋亡率,用均數±標準差表示,統計學分析采用Dunnett t檢驗及q檢驗。結果:瞬時表達各組(PCXN2,NS4B)凋亡率分別為:(918±060)%,(445±053)%。穩定表達各組(PCXN2,NS4B)凋亡率分別為:(1575±209)%,(366±034)%。與PCXN2組比較P lt;001。結論:NS4B抑制肝細胞凋亡率,可能導致肝細胞異常增殖,誘導肝癌發生。
Citation：
XU Jianyu,LI Changping,CHEN Feng,et al. Effect of NonStructural Protein 4B in Hepatitis C Virus on Apoptosis in Hepatic Cells. West China Medical Journal, 2009, 24(5): 1165-1167. doi:
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