低氧肺動脈高壓大鼠對紅細胞增多的適應_《華西醫學》

作者：

四川大學1.華西醫院心臟外科;2.華西第二醫院小兒心血管科,四川成都 610041;

關鍵詞：

高原適應人重組促紅細胞生成素肺血管重構紅細胞增多

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目的:研究低氧性肺動脈高壓大鼠對實驗性紅細胞增多的適應。方法:健康SD大鼠28只,體重200～250 g,隨機分為4組:常氧對照組（N）、單純低氧組（H）、低氧+低劑量人重組促紅細胞生成素（rEPO） 600 u/kg（H+E1）組、低氧+高劑量rEPO 1200 u/kg（H+E2）組,每組7只大鼠。除常氧對照組外各低氧組大鼠均缺氧21 d,每日8 h。其中后兩組每周腹部皮下注射不同劑量的rEPO三次。取血樣測定紅細胞數、全血粘度及紅細胞變形指數；頸外靜脈插管測定平均肺動脈壓力；光鏡觀察反映肺動脈重構程度的形態學參數肺小動脈管壁厚度百分比、肺非肌性小動脈肌化程度。結果:①隨著rEPO注射劑量的增加,紅細胞、全血粘度有不同程度的增高；②全血粘度增高的同時紅細胞變形指數也相應地增加；③隨著rEPO劑量的增加,平均肺動脈壓力逐漸增高,但是肺血管重構程度反而有所緩解。結論:實驗性紅細胞增多通過改變紅細胞變形性和緩解肺血管重構程度來阻遏低氧性肺動脈高壓的進一步發展。

引用本文： 李煒,周艷榮,安琪,許紅波. 低氧肺動脈高壓大鼠對紅細胞增多的適應. 華西醫學, 2009, 24(3): 680-683. doi: 復制

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1. GIRGIS R E, MOZAMME L S,CHAMPION H C, et al.Regression of chronic hypoxic pulmonary hypertension by simvastatin[J].Am J Physiol Lung Cell Mol Physiol，2007,2（10）:L1105L1110..
2. NAEYE RL.Polycythemia and hypoxia.Individual effects on heart and pulmonary arteries[J].Am J Pathol,1967, 50（6）:1027-1033.
3. SWIGART RH.Polycythemia and right ventricular hypertrophy[J].Circ Res, 1965,17（8）:30-37..
4. SANTOLAYA RB, LAHIRI S, ALFARO RT，et al.Respiratory adaptation in the highest inhabitants and highest Sherpa mountaineers[J].Respir Physiol,1989,77（2）: 253-262..
5. SHIGA T, MAEDA N, KON K.Erythrocyte rheology[J].Crit Rev Oncol Hematol, 1990, 10（1）:9-48..
6. VOGEL J, KIESSLING I, HEINICKE K,et al.Transgenic mice overexpressing erythropoietin adapt to excessive erythrocytosis by regulating blood viscosity[J].Blood, 2003, 102（6）: 2278-2284..
7. ASAUMI Y, KAGAYA Y, TAKEDA M,et al.Protective role of endogenous erythropoietin system in nonhematopoietic cells against pressure overload–induced left ventricular dysfunction in mice[J].Circulation,2007,115（15）:2022-2032..
8. SATOH K, KAGAYA Y, NAKANO M,et al.Important role of endogenous erythropoietin system in recruitment of endothelial progenitor cells in hypoxiainduced pulmonary hypertension in mice[J].Circulation,2006,113（11）: 1442–1450..
9. BAHLMANN FH, DE GROOT K, SPANDAU JM,et al.Erythropoietin regulates endothelial progenitor cells[J].Blood, 2004,103（3）: 921–926.
10. NAKANO M, SATOH K, FUKUMOTO Y, et al. Important role of erythropoietin receptor to promote VEGF expression and angiogenesis in peripheral lschemia in mice[J].Circ Res,2007,100（5）:662-669..
11. HASEGAWA J,WAGNER K F,KARP D, et al. Altered pulmonary vascular reactivity in mice with excessive erythrocytosis[J].Respir Crit Care Med,2004,169（7）:829-835..

《華西醫學》

低氧肺動脈高壓大鼠對紅細胞增多的適應

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《華西醫學》

低氧肺動脈高壓大鼠對紅細胞增多的適應

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