食管鱗狀細胞癌CyclinD1的表達及其與CyclinE的相關性研究_《華西醫學》

作者：

黃漢興 ¹ , 張金添 ²

1 莆田市第一醫院病理科（福建莆田，351100）; 2 莆田學院醫學院病理教研室;

關鍵詞：

食管癌 CyclinD1 CyclinE 免疫組織化學定量分析

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【摘要】目的 觀察CyclinD1在食管鱗狀細胞癌和癌周正常食管組織的表達，從量化角度闡明其在食管鱗狀細胞癌組織中表達的病理學意義及其與CyclinE表達的關系。方法用免疫組化MaxVision法檢測CyclinD1、CyclinE在食管鱗狀細胞癌和癌周正常食管組織的表達，Image-pro plus圖像分析軟件對其表達強度進行定量分析，并用表達的陽性單位（positive unit，PU）反映其表達強度。結果 CyclinD1蛋白在食管鱗狀細胞癌癌細胞核中表達的PU值高于正常食管組織（P lt;0.001）；CyclinD1蛋白表達的PU值與食管鱗狀細胞癌分化程度有關（P lt;0.001）；浸潤漿膜層組的CyclinD1蛋白表達的PU值高于無漿膜層浸潤組（P=0.037）；伴淋巴結轉移組的CyclinD1蛋白表達的PU值高于無淋巴結轉移組（P=0.012）；CyclinD1與CyclinE蛋白在食管鱗狀細胞癌中表達呈正相關（r=0.650，P lt;0.01）。結論 CyclinD1在食管鱗狀細胞癌中表達上調，且與生物學特性相關，可作為判斷腫瘤患者預后不良的標志之一；CyclinD1和CyclinE可能在食管癌癌變過程中起協同作用。
/【Abstract】　Objective　 To observe the expression of CyclinD1 and its correlation with the expression of CyclinE in esophagus squamous carcinoma.　Methods　 The expressions of CyclinD1 and CyclinE protein were detected by immunohistochemistry (MaxVision method) using the monoclonal antibody and their intensities were assessed quantitatively by Image-pro plus image analysis system.　Results　 The monoclonal antibody was detected in specific cell nucleus. The positive unit (PU) value of CyclinD1 was larger in the esophagus squamous carcinoma than that in the normal esophagus tissue with a statistically significant difference (P lt;0.001). The expression of CyclinD1 was correlated with differentiation of esophagus squamous carcinoma (P lt;0.001) and infiltration degree of esophagus squamous carcinoma (P=0.037). The PU value of CyclinD1 of the nuclei was larger in esophagus squamous carcinoma with lymph node metastasis than those without lymph node metastasis (P=0.012). There was a positive correlation between CyclinD1 protein and CyclinE protein (r=0.650, P lt;0.01).　Conclusion　 The positive expression of CyclinD1 is up-regulated in the esophagus squamous carcinoma. CyclinD1 protein relates to the clinicopathological characteristics and prognostic value in esophagus squamous carcinoma. CyclinD1 expression is bly associated with CyclinE expression in esophagus squamous carcinoma.

引用本文： 黃漢興,張金添. 食管鱗狀細胞癌CyclinD1的表達及其與CyclinE的相關性研究. 華西醫學, 2010, 25(11): 1944-1946. doi: 復制

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6.	Tsai-Turton M, Luong BT, Tan Y, et al. Cyclophosphamide-induced apoptosis in COV434 human granulose cells involves oxidative stress and glutathione depletion[J]. Toxicol Sci, 2007, 98(1): 216-230.
7.	Bilalovic N, Vranic S, Basic H, et al. Immunohistochemical evaluation of CyclinD1 in breast cancer[J]. Croat Med J, 2005, 46(3): 382-384.
8.	Zhu SC, Li R, Wang YX, et al. Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer possible incorporation into clinical TNM staging system[J]. World J Gastroenterol, 2005, 11(25): 3823-3829.
9.	胡玉林, 劉志輝, 趙于天, 等. P53和CyclinD1蛋白的表達對食管癌預后意義的研究[J]. 哈爾濱醫科大學學報, 2007, 41(1): 52-54.
10.	金春亭, 張林西, 李海軍, 等. 人食管鱗癌中細胞周期調節基因蛋白的表達[J]. 世界華人消化雜志, 2005, 13(15): 1909-1912.
11.	Fu ZJ, Ma ZY, Wang QR, et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients[J]. Clin Exp Metastasis, 2008, 25(8): 887-892.
12.	Bahnassy AA, Zekri AR, Houssinis EL, et al. CyclinA and cyclinD, as significant prognostic markers in colorectal patients[J]. BMC Gastroenteral, 2004, 4(1): 22.
13.	田冬艷, 王德華, 劉永紅, 等. Survivin和CyclinD1在卵巢癌中的表達和相關性[J]. 天津醫科大學學報, 2009, 15(1): 81-87.
14.	Shao J, Teraishi F, Katsuda K, et al. p53 inhibits adriamycin-induced down-regulation of cyclin D1 expression in human cancer cells[J]. Biochem Biophys Res Commun, 2002, 290(3): 1101-1107.

1. 　申洪, 陸藥丹. 免疫組織化學染色的定量方法研究[J]. 生物醫學工程學雜志, 1993, 10(3): 281-284.
2. 　申洪. 免疫組織顯色反應強度定量方法研究(Ⅱ)[J]. 細胞與分子免疫學雜志, 1994, 10(4): 33-35.
3. 　申洪. 免疫組織化學染色的定量方法研究(Ⅲ)[J]. 中國組織化學與細胞化學雜志, 1995, 4(1): 89-92.
4. 　Donnellan R, Chetty R. CyclinD1 and human neoplasia[J]. Mol Pathol, 1998, 51(1): l-7.
5. 　Liu B, Ren Z, Shi Y, et al. Activation of signal transducers and activators of transcription 3 and overexpression of its target gene CyclinD1 in laryngeal carcinomas[J]. Laryngoscope, 2008, 118(11): 1976-1980.
6. 　Tsai-Turton M, Luong BT, Tan Y, et al. Cyclophosphamide-induced apoptosis in COV434 human granulose cells involves oxidative stress and glutathione depletion[J]. Toxicol Sci, 2007, 98(1): 216-230.
7. 　Bilalovic N, Vranic S, Basic H, et al. Immunohistochemical evaluation of CyclinD1 in breast cancer[J]. Croat Med J, 2005, 46(3): 382-384.
8. 　Zhu SC, Li R, Wang YX, et al. Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer possible incorporation into clinical TNM staging system[J]. World J Gastroenterol, 2005, 11(25): 3823-3829.
9. 　胡玉林, 劉志輝, 趙于天, 等. P53和CyclinD1蛋白的表達對食管癌預后意義的研究[J]. 哈爾濱醫科大學學報, 2007, 41(1): 52-54.
10. 　金春亭, 張林西, 李海軍, 等. 人食管鱗癌中細胞周期調節基因蛋白的表達[J]. 世界華人消化雜志, 2005, 13(15): 1909-1912.
11. 　Fu ZJ, Ma ZY, Wang QR, et al. Overexpression of CyclinD1 and underexpression of p16 correlate with lymph node metastases in laryngeal squamous cell carcinoma in Chinese patients[J]. Clin Exp Metastasis, 2008, 25(8): 887-892.
12. 　Bahnassy AA, Zekri AR, Houssinis EL, et al. CyclinA and cyclinD, as significant prognostic markers in colorectal patients[J]. BMC Gastroenteral, 2004, 4(1): 22.
13. 　田冬艷, 王德華, 劉永紅, 等. Survivin和CyclinD1在卵巢癌中的表達和相關性[J]. 天津醫科大學學報, 2009, 15(1): 81-87.
14. 　Shao J, Teraishi F, Katsuda K, et al. p53 inhibits adriamycin-induced down-regulation of cyclin D1 expression in human cancer cells[J]. Biochem Biophys Res Commun, 2002, 290(3): 1101-1107.

《華西醫學》

食管鱗狀細胞癌CyclinD1的表達及其與CyclinE的相關性研究

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《華西醫學》

食管鱗狀細胞癌CyclinD1的表達及其與CyclinE的相關性研究

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