Comparison between Instrument Method and Microscope Test Method in Measuring the Percentage of Immature Granulocytes of Peripheral Venous Blood in Patients with Leucocythemia_West China Medical Journal

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Department of Clinical Laboratory, the First Hospital of Qiqihar, Qiqihar, Heilongjiang 161005, P.R. China;

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Keywords：

幼稚粒細胞; 儀器法; 鏡檢法; 白血病

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【摘要】目的 探討儀器法和鏡檢法計數白血病幼稚粒細胞百分比的相關性和一致性。方法 2009年6-9月對71例慢性粒細胞性白血病（慢粒）、亞急性粒細胞性白血病（M2）、急性早幼粒細胞性白血病（M3）及急性粒單核細胞性白血病（M4）白血病患者進行儀器法和鏡檢法計數周圍靜脈血幼稚粒細胞的百分比的檢測，并進行比較分析。結果兩種方法計數的慢粒、M2、M3及M4型共71例白血病患者的幼稚粒細胞的百分比比較，有統計學意義（t=6.404，P lt;0.01）；但具有相關性（r=0.771，P lt;0.001）。且四種類型白血病中的每一種類型的白血病的兩種方法的幼粒值也都具有相關性和不一致性（P lt;0.05）。結論 SYSMEX XE-2100全自動血細胞分析儀對慢粒、M2、M3及M4的周圍靜脈血幼稚粒細胞識別能力欠佳，仍需采用鏡檢法進行檢測。
【Abstract】 Objective To investigate the correlation and consistency of the percentage of immature granulocytes of peripheral venous blood in patients with leucocythemia between the instrument method and microscope test method. Methods From June to September 2009, instrument method and microscope test method were used to measure the percentage of immature granulocytes of peripheral venous blood in patients with leucocythemia [chronic granulocytic leukemia (CGL), subacute granulocytic leukemia (M2), acute promyelocyte leukemia (M3), and acute myelomonocytic leukemia (M4)]. Results The difference in the percentage of immature granulocytes of the 71 samples between the 2 methods was significant (t=6.404,P lt;0.01), but still with correlation (r=0.771,P lt;0.001). Besides, there were also correlation and consistency of the percentage of immature granulocytes of the four types of leukemia between the two methods (P lt;0.05). Conclusion The identification ability of SYSMEX XE-2100 type automatic blood cell analyzer for measuring the percentage of immature granulocytes of peripheral venous blood in patients with CGL, M2, M3 and M4 is still weak. Currently, microscope test method still needs to be applied in the measurement.

Citation： YU Zengfeng,ZHANG Yueju,JIANG Zhenzhong. Comparison between Instrument Method and Microscope Test Method in Measuring the Percentage of Immature Granulocytes of Peripheral Venous Blood in Patients with Leucocythemia. West China Medical Journal, 2010, 25(9): 1691-1692. doi: Copy

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1. 王小林, 王宇, 吳永華. XE-2100全自動血細胞分析儀自動幼稚粒細胞計數與顯微鏡方法比較[J]. 現代檢驗醫學雜志, 2006, 21(6): 62-63.
2. 葉應嫵, 王毓三, 申子瑜. 全國臨床檢驗操作規程[M]. 3版. 南京: 東南大學出版社, 2006: 133-134.
3. 陳文彬, 潘祥林. 診斷學[M]. 6版. 北京: 人民衛生出版社, 2006: 291.
4. 王曉忠, 曾學輝, 莫莉. Sysmex XE-2100血細胞分析儀定量分析外周血幼稚粒細胞性能評價[J]. 實驗與檢驗醫學, 2009, 27(3): 214-216.
5. 朱曉輝, 馬紅玉, 朱忠勇. 應用血液學分析以后顯微鏡復檢標準芻議[J]. 中國實驗診斷學, 2006, 10（3): 296-298.
6. Barnes PW, Mcfadden SL, Machin SJ, et al. The International Consensus Croup for Hematology Review: suggested criteria for action following automated CBC and WBC differential analysis[J]. Lab Hematol, 2005, 11(2): 83-90.
7. 戴澤宇, 金紅, 謝鑫友, 等. 血細胞分析儀應用中鏡檢標本篩選條件的探討[J]. 檢驗醫學, 2004, 19(4): 366.
8. 孫芾, 王厚芳, 于俊峰, 等. 血細胞顯微鏡復檢標準的判定及臨床應用[J]. 中華檢驗醫學雜志, 2005, 28(2): 155.

West China Medical Journal

Comparison between Instrument Method and Microscope Test Method in Measuring the Percentage of Immature Granulocytes of Peripheral Venous Blood in Patients with Leucocythemia

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