B細胞成熟抗原在系統性紅斑狼瘡患者外周血單個核細胞的表達_《華西醫學》

作者：

熊金河 ^1,2 , 胡紹先 ¹

1 華中科技大學同濟醫學院附屬同濟醫院風濕免疫科（武漢，430030）;2 遂寧市中心醫院風濕免疫科;

關鍵詞：

系統性紅斑狼瘡 B細胞成熟抗原外周血單個核細胞

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

【摘要】目的 檢測B細胞成熟抗原（BCMA）mRNA在系統性紅斑狼瘡（SLE）患者外周血單個核細胞（PBMC）的表達水平，探討BCMA在SLE發病中的意義。方法納入2006年1-11月收治的36例SLE患者，同期17例健康志愿者作為對照組，采用半定量RT-PCR法檢測外周血單個核細胞中BCMA mRNA的表達，并與SLE疾病活動指數（SLEDAI）進行相關性分析。結果 SLE患者組BCMA mRNA表達水平（0.598±0.230）均明顯高于正常對照組（0.411±0.309）（P lt;0.05）。SLE患者BCMA mRNA表達水平與SLEDAI評分無相關性（P=0.590）。結論 SLE患者BCMA mRNA表達水平的增高，可能在SLE的發病機制中具有一定的作用。
【Abstract】 Objective To detect the mRNA expression of B-cell maturation antigen (BCMA) in peripheral blood mononuclear cells (PBMC) in patients with systemic lupus erythematosus (SLE), and explore the role of BCMA in the pathogenesis of SLE. Methods From January 2006 to November 2006 the expression of BCMA mRNA in PBMC of 36 patients with SLE and 17 normal controls were measured by half-quantitative RT-PCR. The linear correlation between the expression of BCMA mRNA and SLE disease activity index (SLEDAI) was assessed. Results The level of BCMA mRNA (0.598±0.230) in PBMC significantly increased in SLE patients compared with that in the normal controls (0.411±0.309) (P lt;0.05). The expression of BCMA mRNA in SLE patients showed no correlation with SLEDAI score (P=0.590). Conclusion The results suggest that the expression of BCMA mRNA might play an important role in the pathogenesis of SLE.

引用本文： 熊金河,胡紹先. B細胞成熟抗原在系統性紅斑狼瘡患者外周血單個核細胞的表達. 華西醫學, 2010, 25(8): 1418-1420. doi: 復制

1.	Smith CA, Farrah T, Goodwin RG. The TNF receptor super family of cellular and viral proteins: activation, costimulation, and death[J]. Cell, 1994, 76(6): 959-962.
2.	Morel J, Roubille C, Planelles L, et al. Serum levels of tumour necrosis factor family members a proliferation-inducing ligant (APRIL) and B lymphocyte stimulator (BLYS) are inversely correlated in systemic lupus erythematosus[J].Ann Rheum Dis, 2009, 68(6): 997-1002.
3.	Hong SD, Reiff A, Yang HT, et al. B lymphocyte stimulator expression in pediatric systemic lupus erythematosus and juvenile idiopathic arthritis patients[J].Arthritis Rheum, 2009, 60(11): 3400-3409.
4.	Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells[J]. J Immunol, 2004, 173(2): 807-817.
5.	Patel DR, Wallweber HJ, Yin J, et al. Engineering an APRIL-specific B cell maturation antigen[J].J Biol Chem, 2004, 279(16): 16727-16735.
6.	Roschke V, Sosnovtseva S, Ward CD, et al. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic disease[J]. J Immunol, 2002, 169(8): 4314-4321.
7.	Mackay F, Ambrose C. The TNF family members BAFF and APRIL: the growing complexity[J]. Cytokine Growth Factor Rev, 2003, 14(3-4): 311-324.
8.	Hendriks J, Planelles L, de Jong-Odding J, et al. Heparan sulfate proteoglycan binding promotes APRIL-induced tumor cell proliferation[J]. Cell Death Differ, 2005, 12(6), 637-648.
9.	Melchers F. Actions of BAFF in B cell maturation and its effects on the development of autoimmune disease[J].Ann Rheum Dis, 2003, 62(Suppl 2): 25-27.

1. Smith CA, Farrah T, Goodwin RG. The TNF receptor super family of cellular and viral proteins: activation, costimulation, and death[J]. Cell, 1994, 76(6): 959-962.
2. Morel J, Roubille C, Planelles L, et al. Serum levels of tumour necrosis factor family members a proliferation-inducing ligant (APRIL) and B lymphocyte stimulator (BLYS) are inversely correlated in systemic lupus erythematosus[J].Ann Rheum Dis, 2009, 68(6): 997-1002.
3. Hong SD, Reiff A, Yang HT, et al. B lymphocyte stimulator expression in pediatric systemic lupus erythematosus and juvenile idiopathic arthritis patients[J].Arthritis Rheum, 2009, 60(11): 3400-3409.
4. Ng LG, Sutherland AP, Newton R, et al. B cell-activating factor belonging to the TNF family (BAFF)-R is the principal BAFF receptor facilitating BAFF costimulation of circulating T and B cells[J]. J Immunol, 2004, 173(2): 807-817.
5. Patel DR, Wallweber HJ, Yin J, et al. Engineering an APRIL-specific B cell maturation antigen[J].J Biol Chem, 2004, 279(16): 16727-16735.
6. Roschke V, Sosnovtseva S, Ward CD, et al. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic disease[J]. J Immunol, 2002, 169(8): 4314-4321.
7. Mackay F, Ambrose C. The TNF family members BAFF and APRIL: the growing complexity[J]. Cytokine Growth Factor Rev, 2003, 14(3-4): 311-324.
8. Hendriks J, Planelles L, de Jong-Odding J, et al. Heparan sulfate proteoglycan binding promotes APRIL-induced tumor cell proliferation[J]. Cell Death Differ, 2005, 12(6), 637-648.
9. Melchers F. Actions of BAFF in B cell maturation and its effects on the development of autoimmune disease[J].Ann Rheum Dis, 2003, 62(Suppl 2): 25-27.

《華西醫學》

B細胞成熟抗原在系統性紅斑狼瘡患者外周血單個核細胞的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《華西醫學》

B細胞成熟抗原在系統性紅斑狼瘡患者外周血單個核細胞的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料