【摘要】 目的  探討甘油三酯（triglyceride，TG）和總膽固醇（total cholesterol，TC）試劑對血清總膽汁酸（total bile acid，TBA）檢測結果的影響及解決辦法。 方法  2008年1月-2009年10月采用魅力2000全自動生化分析儀，首先單獨檢測20份血清標本的TBA 含量。然后分別檢測TC和TG后進行TBA含量檢測。最后設定特殊檢測程序和清洗程序后再按TC→TBA，TG→TBA順序進行TBA含量檢測。 結果 單獨檢測20份血清標本的TBA結果均值為7.2 μmol/L;按TC→TBA，TG→TBA順序檢測結果均值分別為13.5 μmol/L和14.3 μmol/L，單獨和組合測量方法測定TBA結果有統計學意義（P lt;0.05）。設定特殊檢測程序和清洗程序后按TC→TBA，TG→TBA順序檢測結果均值分別為7.4 μmol/L和7.5 μmol/L，與單獨測量TBA結果相比，無統計學意義。 結論  TG和TC試劑對TBA檢測產生干擾的原因是試劑成分中含有濃度較高的TBA，在魅力2000全自動生化分析儀上，設定特殊檢測程序和清洗程序，能有效消除TG、TC試劑對TBA檢測的影響。
【Abstract】 Objective  To explore the influence and procedure improvement strategies of triglyceride (TG) and cholesterol reagent on the result of serum total bile acid (TBA) test. Methods  The study was carried out between January 2008 to October 2009. The charming 2000 automatic biochemical analyzer was used. First, TBA content of 20 serum samples was independently tested. Then TBA was detected after the detection of TG and total cholesterol (TC) concentration. Finally, TBA test was conducted with special testing procedures and cleaning procedures of TC→TBA and TG→TBA method. Results  The average concentrate of TBA of 20 serum samples was 7.2 μmol/L, while the average concentrates of TBA tested by TC→TBA and TG→TBA methods were 13.5 μmol/L and 14.3 μmol/L, respectively. With special testing procedures and cleaning procedures of TC→TBA and TG→TBA tests, the average concentrates of TBA were 7.4 μmol/L and 7.5 μmol/L, respectively. Conclusion  High concentration of TBA in triglyceride and cholesterol reagents contributes substantially to the interference in serum total bile acids detection. With the charming 2000 automatic biochemical analyzer, special testing procedures and cleaning procedures are able to effectively eliminate the interference of triglycerides and cholesterol reagents from TBA detection.

引用本文： 崔軍. 甘油三酯和總膽固醇試劑對總膽汁酸檢測結果的影響. 華西醫學, 2010, 25(7): 1328-1329. doi: 復制