【摘要】 目的 建立血液中乙醇的直接稀釋-填充柱氣相色譜測定法，將其與現行推薦方法GA/T105-1995進行對比，同時對不同采血管對乙醇含量的影響進行研究。 方法 使用GDX-102填充柱作為分析柱，柱溫160 ℃，汽化室190 ℃，檢測器（FID）190 ℃；用1 mg/mL正丙醇溶液稀釋血液50倍，經離心后，取上清液1 μL進樣測定。 結果 本法回收率91.2%~105.7%，與GA/T105-1995推薦方法測定結果最大相對誤差為7.1%，血液保存于非抗凝管的血醇濃度比抗凝管稍高。 結論 該法適用于血液中乙醇含量的測定，樣品處理更簡便。不同采血管對血醇含量有一定影響，綜合考慮各因素后建議使用枸櫞酸鈉抗凝管作為采血管。
【Abstract】 Objective To establish a direct dilution-gas chromatographic method for the determination of ethanol in blood, compare the method with GA/T105-1995 recommendation method, and study the effects of blood tubes with different anticoagulants on the ethanol contents. Methods GDX-102 packed column was used as separation column with an oven temperature of 160 ℃, an injector temperature of 190 ℃ and a flame ionization detector temperature of 190 ℃. Normal propanol solution at 1 mg/mL was adopted to dilute the samples with a volume 50 times of the propanol solution. After being centrifuged, 1ul of the supernatant liquid was injected for analysis. Results The recovery rate of the method was between 91.2% and 105.7%. The deviation of the method with GA/T105-1995 recommendation method was less than 7.1%. The concentration of blood ethanol preserved in the non-anticoagulant tubes was a little higher than that preserved in the anticoagulant tubes. Conclusions The method can be used for the determination of ethanol content in blood. Compared with GA/T105-1995 recommendation method, the sample treatment of this method is much simpler. And the blood tubes with different anticoagulants have influences on the ethanol contents. It is recommended that blood tubes with sodium citrate as anticoagulant can be used for blood sampling and preservers.
引用本文： 劉迎春,陳杰,吉華貴,孫成均. 直接稀釋—氣相色譜法測定血液中乙醇含量. 華西醫學, 2011, 26(4): 572-575. doi: 復制