參附注射液對大鼠深Ⅱ度燒傷創面愈合的影響_《華西醫學》

作者：

張艷閣 ,  岑瑛

四川大學華西醫院燒傷整形科（成都，610041）;

關鍵詞：

參附注射液燒傷創面愈合抗氧化大鼠

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【摘要】　目的　探討中成藥參附注射液對大鼠深Ⅱ度燒傷創面愈合的影響。　方法　選用16只Sprague-Dawley（SD）大鼠，隨機分為實驗組、對照組，每組各8只，建立20%體表面積深Ⅱ度燙傷模型。燙傷后即刻及此后每天，實驗組大鼠以參附注射液20 mL/kg腹腔注射給藥，1次/d，連續給藥5 d；對照組給予注射等劑量的生理鹽水。分別于致傷后的7、14 d取創面組織塊，光學顯微鏡觀察成纖維細胞及膠原纖維生長情況，電子顯微鏡觀察致傷后14 d成纖維細胞細胞器情況，分別計算燒傷后第7、14天兩組的創面愈合率，并觀察比較兩組創面愈合的時間。　結果　所有選入實驗的SD大鼠均存活至實驗結束。實驗組、對照組術后第7天創面愈合率分別為（36.34±2.55）%及（33.13±2.62）%，兩組差異有統計學意義（t=2.486，P=0.027）；實驗組、對照組術后第14天創面愈合率分別為（75.71±2.29）%及（72.36±2.85）%，兩組差異有統計學意義（t=2.590，P=0.022）；實驗組、對照組創面愈合時間分別為（20.88±1.36）、（22.94±2.16） d，兩組差異有統計學意義（t=-2.286，P=0.0395）。傷后7、14 d創面組織塊切片光學顯微鏡觀察發現，實驗組肉芽組織及膠原纖維較多，排列更有序，表皮基底細胞增殖活躍，炎癥反應較輕；傷后14 d時電子顯微鏡觀察發現，實驗組成纖維細胞的細胞器更豐富，分泌膠原更多，實驗組創面愈合情況優于對照組。　結論　腹腔注射參附注射液可以促進燒傷創面的愈合，其可能的機制為清除氧自由基，抗脂質過氧化。
【Abstract】　Objective　 To explore the effect of Shenfu injection on promoting healing of deep partial-thickness burn wound in rats. 　Methods　 Sixteen Sprague-Dawley (SD) rats were randomly selected, and deep partial-thickness burn with 20% of the body surface was inflicted. The rats were randomly divided into experimental group and control group with eight in each group. Rats in the experimental group were treated with abdominal injection of Shenfu injection at a dose of 20 mL/(kg?d) for five days continually, and rats in the control group were treated with the same dose of 0.9% saline solution. The growth of granulation tissue and collagen fibers were evaluated under light microscope at the seventh and the fourteenth day. The growth of fibroblast was observed under transmission electron microscope at the fourteenth day. The cure rate of both groups of rats at the seventh and fourteenth day was calculated. Then we compared their healing time and the cure rate of the traumatic wound respectively.　Results　 All rats had survived until wound healing. The cure rate at the seventh day for the experimental group and the control group was (36.34±2.55)% and (33.13±2.62)%, and their difference was statistical (t=2.486, P=0.027); At the fourteenth day, the cure rate was respectively (75.71±2.29)% and (72.36±2.85)% with a significant difference between each other (t=2.590, P=0.022). The healing time of the experimental group (20.88±1.36) was significantly shorter than that of the control group (22.9±2.16) (t=-2.286, P=0.040). At the seventh and fourteenth day, light microscope observation showed that the growth of granulation tissue and collagen fibers for rats in the experimental group were much more than that in the control group, the basale cell proliferation was more active, and inflammation was slighter. Through transmission electron microscope, we observed more fibroblast and collagen in the experimental group, which showed a better cure than the control group.　Conclusion　 Shenfu injection can significantly promote wound healing of deep partial-thickness burn. It may possibly get this effect through anti-oxidation.

引用本文： 張艷閣,岑瑛. 參附注射液對大鼠深Ⅱ度燒傷創面愈合的影響. 華西醫學, 2011, 26(4): 547-550. doi: 復制

1.	Clark RA. Cutaneous tissue repair: basic biologic considerations[J]. J Am Acad Dermatol, 1985, 13(5 Pt l): 701-725.
2.	Haycock JW, Ralston DR, Morris B, et al. Oxidative damage to protein and alterations to antioxidant levels in human cutaneous thermal injury[J]. Burns, 1997, 23(7-8): 533-540.
3.	Sayan H, Ozacmak VH, Ozen OA, et al. Beneficial effects of melatonin on reperfusion injury in rat sciatic nerve[J]. J Pineal Res, 2004, 37(3): 143-148.
4.	Thang PT, Patrick S, Teik LS, et al. Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage[J]. Burns, 2001, 27(4): 319-327.
5.	鄭世營, 張曉膺, 王志剛, 等. 參附注射液對離體大鼠缺氧/復氧心肌的保護作用[J]. 中國急救醫學, 2004, 24(6): 425-427.
6.	Cao J, Zheng CD, Zhang GX, et al. Protective effect of Shenfu injection on mitochondria injured by ischemia-reperufsion in Rabbits[J]. Chin Medl J (Engl), 2005, 118(6): 505-507.
7.	孟慶濤, 夏中元, 賈一帆, 等. 參附注射液對大鼠腸缺血再灌注時腸上皮細胞casapses-3、bcl-2基因表達的影響[J]. 武漢大學學報(醫學版), 2004, 25(3): 275-278.
8.	張璟瑜, 唐作磊, 納雪晴, 等. 參附注射液對行顱內動脈瘤夾閉術患者血清SOD, MDA, S-100β蛋白的影響[J]. 華西醫學, 2009, 24(4): 975-977.
9.	Zheng CD, Min S. Cardioprotection of Shenfu Injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Integr Med, 2008, 14(1): 10-16.
10.	李揚, 楊博, 陳紹洋, 等. 參附注射液預處理對大鼠腦缺血-再灌注損傷后HSP70和HSP90 表達的影響[J]. 臨床麻醉學雜志, 2010, 26(1): 51-53.
11.	蘇泓潔, 向小勇, 趙興吉, 等. 參附注射液對大鼠心肌缺血再灌注損傷保護作用的實驗研究[J]. 重慶醫科大學學報, 2010, 35(1): 60-64.
12.	馮世杰, 花蘭女, 金曙雯, 等. 大鼠燙傷模型的制作[J]. 上海第二醫科大學學報, 1995, 15(2): 195-197.
13.	孫同柱, 付小兵, 顧小曼, 等. 重組人表皮生長因子促進兔燙傷創面愈合的實驗研究[J]. 實驗動物科學與管理, 2000, 17(4): 12-14.
14.	Arturson GP. Pathophysiology of the burn wound[J]. Ann Chir Gynaecol, 1980, 69(5): 178-190.
15.	吳靜, 胡森, 汪劍威, 等. 維生素C對燙傷休克大鼠腸內補液時腸組織氧自由基損傷的影響[J]. 解放軍醫學雜志, 2008, 33(6): 646-648.
16.	Tanaka H, Matsuda T, Miyagantani Y, et al. Reduction of resuscitation fluid volumes in severely burned patients using ascorbic acid administration: a randomized, prospective study[J]. Arch Surg, 2000, 135(3): 326-331.
17.	Horton JW. Free radicals and lipid peroxidation mediated injury in burn trauma: the role of antioxidant therapy[J]. Toxicology, 2003, 189(1-2): 75-88.
18.	Vorauer-Uhl K, Fürnschlief E, Wagner A, et al. Topically applied liposome encapsulated superoxide dismutase reduces postburn wound size and edema formation[J]. Eur J Pharm Sci, 2001, 14(1): 63-67.
19.	萬敬枝, 程夢琳, 吳基良. 參附注射液對局灶性腦缺血再灌注大鼠腦組織NO、MDA含量和SOD活性的影響[J]. 中國中醫急癥, 2004, 13(6): 381-382.
20.	吳洋, 夏中元. 參附注射液對腸缺血再灌注大鼠腎組織HO-1與iNOS表達的影響[J]. 中華中醫藥雜志, 2010, 25(7): 1024-1027.
21.	Zhang XJ, Song L, Zhou ZG, et al. Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury[J]. World J Gastroenterol, 2006, 12(27): 4389-4391.

1. 　Clark RA. Cutaneous tissue repair: basic biologic considerations[J]. J Am Acad Dermatol, 1985, 13(5 Pt l): 701-725.
2. 　Haycock JW, Ralston DR, Morris B, et al. Oxidative damage to protein and alterations to antioxidant levels in human cutaneous thermal injury[J]. Burns, 1997, 23(7-8): 533-540.
3. 　Sayan H, Ozacmak VH, Ozen OA, et al. Beneficial effects of melatonin on reperfusion injury in rat sciatic nerve[J]. J Pineal Res, 2004, 37(3): 143-148.
4. 　Thang PT, Patrick S, Teik LS, et al. Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine-xanthine oxidase induced damage[J]. Burns, 2001, 27(4): 319-327.
5. 　鄭世營, 張曉膺, 王志剛, 等. 參附注射液對離體大鼠缺氧/復氧心肌的保護作用[J]. 中國急救醫學, 2004, 24(6): 425-427.
6. 　Cao J, Zheng CD, Zhang GX, et al. Protective effect of Shenfu injection on mitochondria injured by ischemia-reperufsion in Rabbits[J]. Chin Medl J (Engl), 2005, 118(6): 505-507.
7. 　孟慶濤, 夏中元, 賈一帆, 等. 參附注射液對大鼠腸缺血再灌注時腸上皮細胞casapses-3、bcl-2基因表達的影響[J]. 武漢大學學報(醫學版), 2004, 25(3): 275-278.
8. 　張璟瑜, 唐作磊, 納雪晴, 等. 參附注射液對行顱內動脈瘤夾閉術患者血清SOD, MDA, S-100β蛋白的影響[J]. 華西醫學, 2009, 24(4): 975-977.
9. 　Zheng CD, Min S. Cardioprotection of Shenfu Injection against myocardial ischemia/reperfusion injury in open heart surgery[J]. Chin J Integr Med, 2008, 14(1): 10-16.
10. 　李揚, 楊博, 陳紹洋, 等. 參附注射液預處理對大鼠腦缺血-再灌注損傷后HSP70和HSP90 表達的影響[J]. 臨床麻醉學雜志, 2010, 26(1): 51-53.
11. 　蘇泓潔, 向小勇, 趙興吉, 等. 參附注射液對大鼠心肌缺血再灌注損傷保護作用的實驗研究[J]. 重慶醫科大學學報, 2010, 35(1): 60-64.
12. 　馮世杰, 花蘭女, 金曙雯, 等. 大鼠燙傷模型的制作[J]. 上海第二醫科大學學報, 1995, 15(2): 195-197.
13. 　孫同柱, 付小兵, 顧小曼, 等. 重組人表皮生長因子促進兔燙傷創面愈合的實驗研究[J]. 實驗動物科學與管理, 2000, 17(4): 12-14.
14. 　Arturson GP. Pathophysiology of the burn wound[J]. Ann Chir Gynaecol, 1980, 69(5): 178-190.
15. 　吳靜, 胡森, 汪劍威, 等. 維生素C對燙傷休克大鼠腸內補液時腸組織氧自由基損傷的影響[J]. 解放軍醫學雜志, 2008, 33(6): 646-648.
16. 　Tanaka H, Matsuda T, Miyagantani Y, et al. Reduction of resuscitation fluid volumes in severely burned patients using ascorbic acid administration: a randomized, prospective study[J]. Arch Surg, 2000, 135(3): 326-331.
17. 　Horton JW. Free radicals and lipid peroxidation mediated injury in burn trauma: the role of antioxidant therapy[J]. Toxicology, 2003, 189(1-2): 75-88.
18. 　Vorauer-Uhl K, Fürnschlief E, Wagner A, et al. Topically applied liposome encapsulated superoxide dismutase reduces postburn wound size and edema formation[J]. Eur J Pharm Sci, 2001, 14(1): 63-67.
19. 　萬敬枝, 程夢琳, 吳基良. 參附注射液對局灶性腦缺血再灌注大鼠腦組織NO、MDA含量和SOD活性的影響[J]. 中國中醫急癥, 2004, 13(6): 381-382.
20. 　吳洋, 夏中元. 參附注射液對腸缺血再灌注大鼠腎組織HO-1與iNOS表達的影響[J]. 中華中醫藥雜志, 2010, 25(7): 1024-1027.
21. 　Zhang XJ, Song L, Zhou ZG, et al. Effect of shenfu injection on gastrointestinal microcirculation in rabbits after myocardial ischemia-reperfusion injury[J]. World J Gastroenterol, 2006, 12(27): 4389-4391.

《華西醫學》

參附注射液對大鼠深Ⅱ度燒傷創面愈合的影響

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《華西醫學》

參附注射液對大鼠深Ⅱ度燒傷創面愈合的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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