慢性缺氧對大鼠巖神經節神經元酸敏感離子通道亞型3和亞型2a表達的影響_《華西醫學》

作者：

王乾成 , 陳祁 , 楊蔭 , 趙書 , 陳麗 ,  鄭煜

四川大學華西基礎醫學與法醫學院生理學教研室（成都，610041）;

關鍵詞：

巖神經節慢性缺氧酸敏感離子通道大鼠

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【摘要】　目的　探討慢性缺氧對大鼠巖神經節神經元酸敏感離子通道（acid-sensing ion channels，ASICs）亞型3（ASIC3）和亞型2a（ASIC2a）表達的影響。　方法　將12只健康成年SD大鼠隨機分為正常組和缺氧組。用免疫組織化學法（PV）觀察正常和慢性缺氧大鼠巖神經節神經元ASIC3和ASIC2a的表達。　結果　給予慢性缺氧刺激后，巖神經節ASIC3陽性表達神經元數目增多（P lt;0.05），灰度值降低（P lt;0.05）；而ASIC2a陽性表達神經元數目和灰度值無明顯變化（P gt;0.05）。　結論　慢性缺氧可上調大鼠巖神經節神經元ASIC3的表達，而對ASIC2a的表達無明顯影響，提示ASIC3和ASIC2a可能在巖神經節對缺氧的反應中起著不同的作用。
【Abstract】　Objective　 To investigate the effects of chronic hypoxia on expression of acid-sensing ion channels (ASIC) 3 and ASIC2a in neurons of petrosal ganglions of rats.　Methods　 A total of 12 SD rats were randomly assigned to control group and hypoxia group. The expressions of ASIC3 and ASIC2a of the neurons in the petrosal ganglions in the two groups were investigated with the immunohistochemical technique.　Results　 The level of positive ASIC3 expression in the petrosal ganglions was higher in the hypoxia group than that in the control group (P lt;0.05); the difference of positive ASIC2a expression levels between the control group and the hypoxia group was not statistically significant (P gt;0.05).　Conclusion　 Chronic hypoxia can significantly increase the expression of ASIC3, but not that of ASIC2a, of the neurons in the petrosal ganglions, suggesting their different roles in mediating a cellular response to chronic hypoxia.

引用本文： 王乾成,陳祁,楊蔭,趙書,陳麗,鄭煜. 慢性缺氧對大鼠巖神經節神經元酸敏感離子通道亞型3和亞型2a表達的影響. 華西醫學, 2011, 26(2): 170-173. doi: 復制

1.	Reeh PW, Kress M. Molecular physiology of proton transduction in nociceptors[J]. Curr Opin Pharmacol, 2001, 1(1): 45-51.
2.	伍龍軍, 徐樂天. 酸敏感離子通道的功能及其相關調控[J]. 生物化學與生物物理學進展, 2003, 3(3): 339-343.
3.	Duan B, Wu LJ, Yu YQ, et al. Upregulation of acid-sensing ion channel ASIC1a in spinal dorsal horn neurons contributes to inflammatory pain hypersensitivity[J]. J Neurosci, 2007, 27(41): 11139-11148.
4.	Shimada S, Ueda T, Ishida Y, et al. Acid-sensing ion channels in taste buds[J]. Arch Histol Cytol, 2006, 69(4): 227-231.
5.	Fukuda T, Ichikawa H, Terayama R, et al. ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia[J]. Brain Research, 2006, 1081(1): 150-155.
6.	陳祁, 潘際剛, 曹性玲, 等. 酸敏感離子通道在大鼠巖神經節神經元的表達[J]. 華西醫學, 2009, 24(6): 1470-1472.
7.	Babinski K, Catarsi S, Biagini G, et al. Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+[J]. J Biol Chem, 2000, 275 (37): 28519-28525.
8.	Tan ZY, Lu Y, Whiteis CA, et al. Acid-sensing ion channels contribute to transduction of extracellular acidosis in rat carotid body glomus cells[J]. Circ Res, 2007, 101(10): 1009-1019.
9.	李丹, 陳海鋒, 唐玉紅, 等. 酸刺激對大鼠頸動脈體Ⅰ型細胞酸敏感離子通道亞型表達的影響[J]. 生理學報, 2009, 61(1): 43-48.
10.	Sutherland SP, Benson CJ, Adelman JP, et al. Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons[J]. Proc Natl Acad Sci USA, 2001, 98(2): 711-716.
11.	Immke DC, McCleskey EW. ASIC3: a lactic acid sensor for cardiac pain[J]. Sci World J, 2001, 1(1): 510-512.
12.	Johnson MB, Jin K, Minami M, et al. Global ischemia induces expression of acid-sensing ion channel 2a in rat brain[J]. J Cereb Blood Flow Metab, 2001, 21(6): 734-740.
13.	Xu TL, Xiong ZG. Dynamic regulation of acid-sensing ion channels by extracellular and intracellular modulators[J]. Curr Med Chem, 2007, 14(16): 1753-1763.
14.	Mamet J, Lazdunski M, Voilley N. How nerve growth factor drives physiological and inflammatory expressions of acid-sensing ion channel 3 in sensory neurons[J]. J Biol Chem, 2003, 278(49): 48907-48913.
15.	Voilley N, de Weille J, Mamet J, et al. Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors[J]. J Neurosci, 2001, 21(20): 8026-8033.

1. 　Reeh PW, Kress M. Molecular physiology of proton transduction in nociceptors[J]. Curr Opin Pharmacol, 2001, 1(1): 45-51.
2. 　伍龍軍, 徐樂天. 酸敏感離子通道的功能及其相關調控[J]. 生物化學與生物物理學進展, 2003, 3(3): 339-343.
3. 　Duan B, Wu LJ, Yu YQ, et al. Upregulation of acid-sensing ion channel ASIC1a in spinal dorsal horn neurons contributes to inflammatory pain hypersensitivity[J]. J Neurosci, 2007, 27(41): 11139-11148.
4. 　Shimada S, Ueda T, Ishida Y, et al. Acid-sensing ion channels in taste buds[J]. Arch Histol Cytol, 2006, 69(4): 227-231.
5. 　Fukuda T, Ichikawa H, Terayama R, et al. ASIC3-immunoreactive neurons in the rat vagal and glossopharyngeal sensory ganglia[J]. Brain Research, 2006, 1081(1): 150-155.
6. 　陳祁, 潘際剛, 曹性玲, 等. 酸敏感離子通道在大鼠巖神經節神經元的表達[J]. 華西醫學, 2009, 24(6): 1470-1472.
7. 　Babinski K, Catarsi S, Biagini G, et al. Mammalian ASIC2a and ASIC3 subunits co-assemble into heteromeric proton-gated channels sensitive to Gd3+[J]. J Biol Chem, 2000, 275 (37): 28519-28525.
8. 　Tan ZY, Lu Y, Whiteis CA, et al. Acid-sensing ion channels contribute to transduction of extracellular acidosis in rat carotid body glomus cells[J]. Circ Res, 2007, 101(10): 1009-1019.
9. 　李丹, 陳海鋒, 唐玉紅, 等. 酸刺激對大鼠頸動脈體Ⅰ型細胞酸敏感離子通道亞型表達的影響[J]. 生理學報, 2009, 61(1): 43-48.
10. 　Sutherland SP, Benson CJ, Adelman JP, et al. Acid-sensing ion channel 3 matches the acid-gated current in cardiac ischemia-sensing neurons[J]. Proc Natl Acad Sci USA, 2001, 98(2): 711-716.
11. 　Immke DC, McCleskey EW. ASIC3: a lactic acid sensor for cardiac pain[J]. Sci World J, 2001, 1(1): 510-512.
12. 　Johnson MB, Jin K, Minami M, et al. Global ischemia induces expression of acid-sensing ion channel 2a in rat brain[J]. J Cereb Blood Flow Metab, 2001, 21(6): 734-740.
13. 　Xu TL, Xiong ZG. Dynamic regulation of acid-sensing ion channels by extracellular and intracellular modulators[J]. Curr Med Chem, 2007, 14(16): 1753-1763.
14. 　Mamet J, Lazdunski M, Voilley N. How nerve growth factor drives physiological and inflammatory expressions of acid-sensing ion channel 3 in sensory neurons[J]. J Biol Chem, 2003, 278(49): 48907-48913.
15. 　Voilley N, de Weille J, Mamet J, et al. Nonsteroid anti-inflammatory drugs inhibit both the activity and the inflammation-induced expression of acid-sensing ion channels in nociceptors[J]. J Neurosci, 2001, 21(20): 8026-8033.

《華西醫學》

慢性缺氧對大鼠巖神經節神經元酸敏感離子通道亞型3和亞型2a表達的影響

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《華西醫學》

慢性缺氧對大鼠巖神經節神經元酸敏感離子通道亞型3和亞型2a表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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