腹內高壓對肝硬化小鼠肺水通道蛋白1及水通道蛋白5表達的影響_《華西醫學》

作者：

張照杰 ,  楊長青

同濟大學附屬同濟醫院消化科消化疾病研究所（上海，200065）;

關鍵詞：

腹內高壓肝硬化水通道蛋白肺水腫小鼠

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【摘要】　目的　探討腹水引起的腹內高壓對肝硬化小鼠肺組織水通道蛋白1（AQP1）和水通道蛋白5（AQP5）表達的影響。　方法　雄性美國癌癥研究所（Institudo of Cancer Reseach，ICR）小鼠50只，隨機取10只作正常對照組（腹壓0 cm H2O，1 cm H2O=0.098 kPa），其余40只用四氯化碳建立肝硬化小鼠模型，并隨機分為4組:肝硬化（腹壓0 cm H2O）組、肝硬化（腹壓5 cm H2O）組、肝硬化（腹壓10 cm H2O）組、肝硬化（腹壓20 cm H2O）組，通過腹腔注射不同量的白蛋白生理鹽水形成不同的腹壓，并維持腹壓24 h后取肺組織行病理、免疫組織化學、肺濕/干比值及實時熒光定量PCR檢測AQP1和AQP5 mRNA表達量。　結果　與正常對照小鼠相比，肝硬化小鼠肺AQP5、AQP1表達明顯下降（P lt;0.05）；肝硬化小鼠隨著腹內壓的升高，肺濕/干比值升高，AQP5、AQP1表達相應增加（P lt;0.05）。　結論　肝硬化可以影響肺AQP1、AQP5的表達；肝硬化小鼠隨著腹內壓的升高，AQP1、AQP5表達相應增加，并與肺水腫的嚴重程度密切相關。
【Abstract】　Objective　 To investigate the role of intra-abdominal hypertension caused by ascites on the expression of Aquaporin (AQP) 1 and AQP 5 in the lung of cirrhotic mice.　Methods　 We randomly chose 10 from 50 male Institude of Cancer Research (ICR) mice to form the control group [intra-abdominal pressure (IAP)=0 cm H2O, 1 cm H2O=0.098 kPa]. The model of cirrhosis were prepared by subcutaneous injection of carbon tetrachloride for the rest 40 mice which were then randomly divided into 4 groups: cirrhosis (IAP=0 cm H2O) group, cirrhosis (IAP=5 cm H2O) group, cirrhosis (IAP=10 cm H2O) group, and cirrhosis (IAP=20 cm H2O) group. Saline with different volume of albumin was injected into the peritoneum of each mouse in order to form different IAP. After 24 hours, analysis of pathology, immunochemistry and wet/dry ratio was done for the lungs of these mice; and the expression of AQP1 and AQP5 at the protein and mRNA levels were analyzed by IHC and qRT-PCR.　Results　 Compared with the normal mice, the expression of AQP1 and AQP5 in lungs of cirrhotic mice were significantly lower (P lt;0.05). Both the lung wet/dry ratio and the expression of AQP1 and AQP5 raised with the increase of IAP.　Conclusion　 Cirrhosis can affect the expression of AQP1 and AQP5 in lungs. The expression of AQP5 and AQP1 in lungs of cirrhotic mice increases with the increase of IAP, which is also closely correlated with the severity of pulmonary edema.

引用本文： 張照杰,楊長青. 腹內高壓對肝硬化小鼠肺水通道蛋白1及水通道蛋白5表達的影響. 華西醫學, 2011, 26(2): 165-169. doi: 復制

1.	Agre P, Kozono D. Aquaporin water channels molecular mechanisms for human diseases[J]. FEBS Lett, 2003, 27, 555(1): 72-78.
2.	Erik J. Teicher, Micheal D. Pasquale, et al. Abdominal compartment syndrome[J]. Operat Tech Gen Surg, 2008, 10(1): 39-59.
3.	Scheppach W. Abdominal compartment syndrome[J]. Best Pract Res Clin Gastroenterol, 2009, 23(1): 25-33.
4.	Towse JE, Harrod KS, Krane CM, et al. Decreased expression of AQP1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Bio, 2000, 22(1): 34-44.
5.	Ma T, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-5 knockout mice[J]. J Clin Invest, 2000, 105 (1): 93-100.
6.	Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats[J]. Chin Med J, 2002, 115(7): 963-967.
7.	岳冬梅, 薛辛東. 水通道蛋白1, 5與新生鼠高氧肺損傷腦水腫的關系研究[J]. 中國當代兒科雜志, 2006, 2(4): 147-150.
8.	King LS, Agre P. Pathophysiology of the aquaporin-water channel[J]. Ann Rev Physio, 1996, 58: 619-648.
9.	Towse JE, Harrod KS, Krane CM, et al. Decreased expression of AQP1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Bio, 2000, 22(1): 34-44.
10.	Jiao GY, Li ER, Yu RJ. Decreased expression of AQP1 and AQP5 in acute injured lung in rats[J]. Chin Med J, 2002, 115(7): 963-967.
11.	Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice[J]. J Clin Invest, 1999, 103(4): 555-561.
12.	高巨, 招偉賢, 肖建斌, 等. “失血性休克-內毒素”二次打擊所致肺損傷水通道蛋白的表達[J]. 中國急救醫學, 2007, 27(6): 546-549.
13.	謝艷萍, 陳才平, 王建春, 等. 急性肺損傷大鼠肺水通道蛋白1和5表達及功能的實驗研究[J]. 中華結核和呼吸雜志, 2005, 28(6): 385-389.
14.	Song Y. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice[J]. J Physiol, 2000, 15(3): 771-779.
15.	Lai KN, Leung JC, Metz CN, et al. Role for macrophage migration inhibitory factor in acute respiratory distress syndrome[J]. J Patho, 2003, 199(4): 496-508.
16.	Jang AS, Lee JU, Choi IS, et al. Expression of nitric ocide synthase, aquaporin1 and aquaporin5 in rat after bleomycin inhalation[J]. Intensive Care Med, 2004, 30(3): 489-495.

1. 　Agre P, Kozono D. Aquaporin water channels molecular mechanisms for human diseases[J]. FEBS Lett, 2003, 27, 555(1): 72-78.
2. 　Erik J. Teicher, Micheal D. Pasquale, et al. Abdominal compartment syndrome[J]. Operat Tech Gen Surg, 2008, 10(1): 39-59.
3. 　Scheppach W. Abdominal compartment syndrome[J]. Best Pract Res Clin Gastroenterol, 2009, 23(1): 25-33.
4. 　Towse JE, Harrod KS, Krane CM, et al. Decreased expression of AQP1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Bio, 2000, 22(1): 34-44.
5. 　Ma T, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-5 knockout mice[J]. J Clin Invest, 2000, 105 (1): 93-100.
6. 　Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats[J]. Chin Med J, 2002, 115(7): 963-967.
7. 　岳冬梅, 薛辛東. 水通道蛋白1, 5與新生鼠高氧肺損傷腦水腫的關系研究[J]. 中國當代兒科雜志, 2006, 2(4): 147-150.
8. 　King LS, Agre P. Pathophysiology of the aquaporin-water channel[J]. Ann Rev Physio, 1996, 58: 619-648.
9. 　Towse JE, Harrod KS, Krane CM, et al. Decreased expression of AQP1 and AQP5 in mouse lung after acute viral infection[J]. Am J Respir Cell Mol Bio, 2000, 22(1): 34-44.
10. 　Jiao GY, Li ER, Yu RJ. Decreased expression of AQP1 and AQP5 in acute injured lung in rats[J]. Chin Med J, 2002, 115(7): 963-967.
11. 　Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice[J]. J Clin Invest, 1999, 103(4): 555-561.
12. 　高巨, 招偉賢, 肖建斌, 等. “失血性休克-內毒素”二次打擊所致肺損傷水通道蛋白的表達[J]. 中國急救醫學, 2007, 27(6): 546-549.
13. 　謝艷萍, 陳才平, 王建春, 等. 急性肺損傷大鼠肺水通道蛋白1和5表達及功能的實驗研究[J]. 中華結核和呼吸雜志, 2005, 28(6): 385-389.
14. 　Song Y. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice[J]. J Physiol, 2000, 15(3): 771-779.
15. 　Lai KN, Leung JC, Metz CN, et al. Role for macrophage migration inhibitory factor in acute respiratory distress syndrome[J]. J Patho, 2003, 199(4): 496-508.
16. 　Jang AS, Lee JU, Choi IS, et al. Expression of nitric ocide synthase, aquaporin1 and aquaporin5 in rat after bleomycin inhalation[J]. Intensive Care Med, 2004, 30(3): 489-495.

《華西醫學》

腹內高壓對肝硬化小鼠肺水通道蛋白1及水通道蛋白5表達的影響

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《華西醫學》

腹內高壓對肝硬化小鼠肺水通道蛋白1及水通道蛋白5表達的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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