The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro_West China Medical Journal

Authors：

CHEN Jiangli ^1,2 , WANG Xiaodong ¹ , XING Aiyun ¹ ,  LIU Shuyun ¹

1. Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China; 2. Department of Obstetrics and Gynecology, Beijing General Aerospace Hospital, Beijing 100076, P. R. China;

Corresponding?author：

LIU Shuyun, Email: cjl028@163.com

Keywords：

胎盤; 促腎上腺皮質激素釋放激素; 妊娠肝內膽汁淤積癥

Video：

Export PDF Favorites Scan Get Citation

Abstract Full text Figures/Tables Video References Cited by

目的　觀察糖皮質激素對胎盤組織促腎上腺皮質激素釋放激素（CRH）的分泌水平的影響。方法　收集2006年1月－3月住院分娩的正常妊娠婦女的胎盤組織與妊娠肝內膽汁淤積癥（ICP）患者胎盤及其血清各10例。分3組進行胎盤組織培養，即正常胎盤組、ICP胎盤組，正常胎盤組織加ICP患者血清組，分別用放射免疫法測定各組加與不加地塞米松胎盤組織培養液中CRH的水平。結果　正常組與正常胎盤加地塞米松組培養24、48、72、96 h其CRH分泌水平分別為：（74.81 ± 27.92）、（63.71 ± 24.72）、（91.87 ± 41.64）、（98.90 ± 42.52） pg/mL；（66.94 ± 29.62）、（77.39 ± 31.84）、（61.89 ± 33.94）、（75.13 ± 36.98） pg/mL，兩組比較差異有統計學意義（P＞0.05）。ICP組與ICP加地塞米松組培養上清液中CRH水平在24、48、72、96 h其CRH分泌水平分別為：（48.28 ± 16.56）、（60.20 ± 29.97）、（72.92 ± 31.65）、（69.22 ± 29.33）pg/mL；（41.81 ± 25.00）、（57.36 ± 39.75）、（57.72 ± 23.29）、（61.43 ± 20.77）pg/mL, 兩組比較差異有統計學意義（P＞0.05）；正常胎盤加ICP血清培養組與正常胎盤加ICP血清加地塞米松培養組上清液中CRH水平在24、48、72、96 h其CRH分泌水平分別為：（84.9 ± 34.98）、（74.5 ± 29.93）、（71.1 ± 27.26）、（81.0 ± 37.18）pg/mL；（76.29 ± 33.11）、（63.70 ± 24.20）、（64.85 ± 28.39）、（67.65 ± 33.20）pg/mL，兩組比較差異有統計學意義（P＞0.05）。3組加入地塞米松培養的胎盤組織，CRH分泌水平并無明顯改變。結論　地塞米松不影響體外培養胎盤組織CRH分泌。

Citation： CHEN Jiangli,WANG Xiaodong,XING Aiyun,LIU Shuyun. The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro. West China Medical Journal, 2012, 27(8): 1181-1185. doi: Copy

1.	Howard Howard PJ, Muwhy GM. Bile acid stress in the mother and baby unit[J]. Eur J Gastroenterol Hepatol, 2003, 15(3): 317-321．.
2.	Savoius H, Riikonen S, Gylling H, et al. Pregnancy outcome with intrahepatic cholestasis[J]. Acta Obstet Gynecol Scand, 2000, 79(4): 323-325.
3.	Mullally BA, Hansen WF. Intrahepatic cholestasis of pregnancy: review of the literature[J]. J Obstet Gynecol Surv, 2002, 57(1): 47-52.
4.	Challis JRG, Matthews SG, Van Meir C, et al. Current topic: the placental corticotropin-releasing hormone-adrenoco-rticotrop in axis[J]. Placenta, 1995, 16(6): 481.
5.	Malkoski SP, Dorin RI. Composite glucocorticoid regulation at a functionally defined negative glucocorticoid response element of the human corticotropin-releasing hormone gene[J]. Mol Endocrinol, 1999 , 13(10): 1629-1644.
6.	張岫竹, 周繼紅, 蔣建新. 應激過程中糖皮質激素受體對促腎上腺皮質激素釋放激素的調節作用[J]. 國外醫學·外科學分冊, 2002, 29(5): 261-264.
7.	Jones SA, Brooks AN, Challis JR. Steroids modulate corticotropin releasing hormone production in human fetal membrane and placenta[J]. J Clin Endocrinol Metab, 1989, 68(4): 825-830.
8.	Deuster PA, Petrides JS, Singh A, et al. High intensity exercise promotes escape of adrenocorticotropin and cortisol from suppress -ion by dexame thasone: sexually dimorphic responses[J]. J Clin Endocrinol Metab, 1998, 83(9): 3332-3338.
9.	王曉東, 余海燕, 姚強, 等. 胎盤小葉胎兒血管系統CRH及其受體CRH-R1的表達[J]. 四川大學學報（醫學版）, 2005, 36(5): 634-636.
10.	劉　云, 漆洪波, 王勇, 等. 產前地塞米松治療妊娠肝內膽汁瘀積癥[J]. 中國優生與遺傳雜志, 2003, 11(1): 102-134.
11.	時青云, 劉淑蕓, 彭芝蘭. 腫瘤壞死因子和地塞米松對妊娠肝內膽汁淤積癥患者細胞間粘附分子-1及選擇素-P表達的影響[J]. 中華肝臟病雜志, 2002, 10(2): 125-128.
12.	Robinson BG, Emanuel RL, Frim DM, et al. Glucorticoid stimulates expression of corticotropin releasing hormone gene in human placenta[J]. Proc Natl Acad Sci USA , 1988, 85(14): 5244-5248.
13.	Matthews SG. Antenatal glucocorticoids and programming of the developing CNS[J]. Pediatric Res, 2000, 47(3): 291-300.
14.	孫剛. 胎盤內分泌的基礎與臨床[M]. 上海: 第二軍醫大學出版社, 2001: 3-4.
15.	Margioris AN, Brockmann G, Gomez MJ, et al. Paracrine effect of placental corticotropin-releasing hormone[M]// Ludecke D, Chrousos G, Tolis G. Cushing′s syndrome and other hypercortisolemic. New York: Raven Press, 1990: 189-192.
16.	Siler-Khodr TM, Kang IS, Koong MK, et al. The effect of dexamethasone on CRH and prostanoid production from human term placenta[J]. Prostaglandins, 1997, 54(3): 639-653.
17.	張岫竹, 熊健瓊, 劉媛, 等. 大劑量糖皮質激素對下丘腦室旁核神經元促腎上腺皮質激素釋放激素mRNA轉錄及其蛋白表達的調節作用[J]. 中華創傷雜志, 2005, 21(10): 737-740.

1. 　Howard Howard PJ, Muwhy GM. Bile acid stress in the mother and baby unit[J]. Eur J Gastroenterol Hepatol, 2003, 15(3): 317-321．.
2. 　Savoius H, Riikonen S, Gylling H, et al. Pregnancy outcome with intrahepatic cholestasis[J]. Acta Obstet Gynecol Scand, 2000, 79(4): 323-325.
3. 　Mullally BA, Hansen WF. Intrahepatic cholestasis of pregnancy: review of the literature[J]. J Obstet Gynecol Surv, 2002, 57(1): 47-52.
4. 　Challis JRG, Matthews SG, Van Meir C, et al. Current topic: the placental corticotropin-releasing hormone-adrenoco-rticotrop in axis[J]. Placenta, 1995, 16(6): 481.
5. 　Malkoski SP, Dorin RI. Composite glucocorticoid regulation at a functionally defined negative glucocorticoid response element of the human corticotropin-releasing hormone gene[J]. Mol Endocrinol, 1999 , 13(10): 1629-1644.
6. 　張岫竹, 周繼紅, 蔣建新. 應激過程中糖皮質激素受體對促腎上腺皮質激素釋放激素的調節作用[J]. 國外醫學·外科學分冊, 2002, 29(5): 261-264.
7. 　Jones SA, Brooks AN, Challis JR. Steroids modulate corticotropin releasing hormone production in human fetal membrane and placenta[J]. J Clin Endocrinol Metab, 1989, 68(4): 825-830.
8. 　Deuster PA, Petrides JS, Singh A, et al. High intensity exercise promotes escape of adrenocorticotropin and cortisol from suppress -ion by dexame thasone: sexually dimorphic responses[J]. J Clin Endocrinol Metab, 1998, 83(9): 3332-3338.
9. 　王曉東, 余海燕, 姚強, 等. 胎盤小葉胎兒血管系統CRH及其受體CRH-R1的表達[J]. 四川大學學報（醫學版）, 2005, 36(5): 634-636.
10. 　劉　云, 漆洪波, 王勇, 等. 產前地塞米松治療妊娠肝內膽汁瘀積癥[J]. 中國優生與遺傳雜志, 2003, 11(1): 102-134.
11. 　時青云, 劉淑蕓, 彭芝蘭. 腫瘤壞死因子和地塞米松對妊娠肝內膽汁淤積癥患者細胞間粘附分子-1及選擇素-P表達的影響[J]. 中華肝臟病雜志, 2002, 10(2): 125-128.
12. 　Robinson BG, Emanuel RL, Frim DM, et al. Glucorticoid stimulates expression of corticotropin releasing hormone gene in human placenta[J]. Proc Natl Acad Sci USA , 1988, 85(14): 5244-5248.
13. 　Matthews SG. Antenatal glucocorticoids and programming of the developing CNS[J]. Pediatric Res, 2000, 47(3): 291-300.
14. 　孫剛. 胎盤內分泌的基礎與臨床[M]. 上海: 第二軍醫大學出版社, 2001: 3-4.
15. 　Margioris AN, Brockmann G, Gomez MJ, et al. Paracrine effect of placental corticotropin-releasing hormone[M]// Ludecke D, Chrousos G, Tolis G. Cushing′s syndrome and other hypercortisolemic. New York: Raven Press, 1990: 189-192.
16. 　Siler-Khodr TM, Kang IS, Koong MK, et al. The effect of dexamethasone on CRH and prostanoid production from human term placenta[J]. Prostaglandins, 1997, 54(3): 639-653.
17. 　張岫竹, 熊健瓊, 劉媛, 等. 大劑量糖皮質激素對下丘腦室旁核神經元促腎上腺皮質激素釋放激素mRNA轉錄及其蛋白表達的調節作用[J]. 中華創傷雜志, 2005, 21(10): 737-740.

West China Medical Journal

The Effect of Glucocorticoid on the Excretion of Corticotropin-Releasing Hormone in Placental Tissue in vitro

Abstract Full text Figures/Tables Video References Cited by

Format

Content