磷脂酰肌醇3-激酶和磷酸化蛋白激酶B在膀胱尿路上皮癌中的表達及意義_《華西醫學》

作者：

潘衛兵 , 朱斌 , 金巖 , 謝禮仁 , 楊凱鈞

深圳坪山新區人民醫院泌尿外科（廣東深圳，518118）;

關鍵詞：

膀胱尿路上皮癌免疫組織化學磷脂酰肌醇3-激酶磷酸化蛋白激酶B

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　研究磷脂酰肌醇3-激酶（PI3K）和磷酸化蛋白激酶B（p-Akt）在人膀胱尿路上皮癌組織中的表達特征及臨床意義。方法　2005年6月－2010年7月，采用免疫組織化學法檢測40例膀胱尿路上皮癌組織及10例正常膀胱組織PI3K與p-Akt的表達，并對結果進行統計學分析。結果　PI3K和p-Akt在正常膀胱黏膜組織陽性表達率均低于膀胱尿路上皮癌組織中，差異均有統計學意義（P＜0.05）。同一標本中PI3K和p-Akt的表達不具有相關性（r=0.051，P=0.747）。結論　PI3K、p-Akt在膀胱尿路上皮癌中高表達，兩者在膀胱尿路上皮癌中共同促其發展，但其在膀胱尿路上皮癌的預后和進展中的作用尚不明確。

引用本文： 潘衛兵,朱斌,金巖,謝禮仁,楊凱鈞. 磷脂酰肌醇3-激酶和磷酸化蛋白激酶B在膀胱尿路上皮癌中的表達及意義. 華西醫學, 2012, 27(4): 501-503. doi: 復制

1.	Hartmann W, Digon-Söntgerath B, Koch A, et al. Phosphatidylinositol 3’-kinase/AKT signaling is activated in medulloblastoma cell proliferation and is associated with reduced expression of PTEN[J]. Clin Cancer Res, 2006, 12(10): 3019-3027.
2.	Nyåkern M, Tazzari PL, Finelli C, et al. Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral blood mononuclear cells from high-risk myelodysplastic syndrome patients[J]. Leukemia, 2006, 20(2): 230-238.
3.	Gao N, Flynn DC, Zhang Z, et al. G1 cell cycle progression and the expression of G1 cyclins are regulated by PI3K/AKT/mTOR/p70S6K1 signaling in human ovarian cancer cells[J]. Am J Physiol Cell Physiol, 2004, 287(2): C281-C291.
4.	Chang F, Lee JT, Navolanic PM, et al. Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapy[J]. Leukemia, 2003, 17(3): 590-603.
5.	Skinner HD, Zheng JZ, Fang J, et al. Vascular endothelial growth factor transcriptional activation is mediated by hypoxia-inducible factor 1alpha, HDM2, and p70S6K1 in response to phosphatidylinositol 3-kinase/AKT signaling[J]. Biol Chem, 2004, 279(44): 45643-45651.
6.	Jiang BH, Liu LZ. AKT signaling in regulating angiogenesis[J]. Curr Cancer Drug Targets, 2008, 8(1): 19-26.
7.	Engelman JA. Targeting PI3K signalling in cancer: opportunities, challenges and limitations[J]. Nat Rev Cancer, 2009, 9(8): 550-562.
8.	Martelli AM, Tabellini G, Borgatti P, et al. Nuclear lipids: new functions for old molecules?[J]. J Cell Biochem, 2003, 88(3): 455-461.
9.	Vanhaesebroeck B, Leevers SJ, Ahmadi K, et al. Synthesis and function of 3-phosphorylated inositol lipids[J]. Annu Rev Biochem, 2001, 70(11): 535-602.
10.	Hanada M, Feng J, Hemmings BA. Structure, regulation and function of PKB/AKT--a major therapeutic target[J]. Biochim Biophys Acta, 2004, 1697(1-2): 3-16.
11.	Eblin KE, Bredfeldt TG, Buffington S, et al. Mitogenic signal transduction caused by monomethylarsonous acid in human bladder cells: role in arsenic-induced carcinogenesis[J]. Toxicol Sci, 2007, 95(2): 321-330.
12.	Qiao M, Sheng S, Pardee AB. Metastasis and AKT activation[J]. Cell Cycle, 2008, 7(19): 2991-2996.
13.	Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation[J]. Biochem J, 1998, 335( Pt 1): 1-13.
14.	Song G, Ouyang GL, Bao SD. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med, 2005, 9(1): 59-71.
15.	費洪榮, 王鳳澤. PI3K/Akt信號通路的調控與腫瘤血管生成[J]. 生命的化學, 2010, 30(1): 38-41.

1. 　Hartmann W, Digon-Söntgerath B, Koch A, et al. Phosphatidylinositol 3’-kinase/AKT signaling is activated in medulloblastoma cell proliferation and is associated with reduced expression of PTEN[J]. Clin Cancer Res, 2006, 12(10): 3019-3027.
2. 　Nyåkern M, Tazzari PL, Finelli C, et al. Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral blood mononuclear cells from high-risk myelodysplastic syndrome patients[J]. Leukemia, 2006, 20(2): 230-238.
3. 　Gao N, Flynn DC, Zhang Z, et al. G1 cell cycle progression and the expression of G1 cyclins are regulated by PI3K/AKT/mTOR/p70S6K1 signaling in human ovarian cancer cells[J]. Am J Physiol Cell Physiol, 2004, 287(2): C281-C291.
4. 　Chang F, Lee JT, Navolanic PM, et al. Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapy[J]. Leukemia, 2003, 17(3): 590-603.
5. 　Skinner HD, Zheng JZ, Fang J, et al. Vascular endothelial growth factor transcriptional activation is mediated by hypoxia-inducible factor 1alpha, HDM2, and p70S6K1 in response to phosphatidylinositol 3-kinase/AKT signaling[J]. Biol Chem, 2004, 279(44): 45643-45651.
6. 　Jiang BH, Liu LZ. AKT signaling in regulating angiogenesis[J]. Curr Cancer Drug Targets, 2008, 8(1): 19-26.
7. 　Engelman JA. Targeting PI3K signalling in cancer: opportunities, challenges and limitations[J]. Nat Rev Cancer, 2009, 9(8): 550-562.
8. 　Martelli AM, Tabellini G, Borgatti P, et al. Nuclear lipids: new functions for old molecules?[J]. J Cell Biochem, 2003, 88(3): 455-461.
9. 　Vanhaesebroeck B, Leevers SJ, Ahmadi K, et al. Synthesis and function of 3-phosphorylated inositol lipids[J]. Annu Rev Biochem, 2001, 70(11): 535-602.
10. 　Hanada M, Feng J, Hemmings BA. Structure, regulation and function of PKB/AKT--a major therapeutic target[J]. Biochim Biophys Acta, 2004, 1697(1-2): 3-16.
11. 　Eblin KE, Bredfeldt TG, Buffington S, et al. Mitogenic signal transduction caused by monomethylarsonous acid in human bladder cells: role in arsenic-induced carcinogenesis[J]. Toxicol Sci, 2007, 95(2): 321-330.
12. 　Qiao M, Sheng S, Pardee AB. Metastasis and AKT activation[J]. Cell Cycle, 2008, 7(19): 2991-2996.
13. 　Coffer PJ, Jin J, Woodgett JR. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation[J]. Biochem J, 1998, 335( Pt 1): 1-13.
14. 　Song G, Ouyang GL, Bao SD. The activation of Akt/PKB signaling pathway and cell survival[J]. J Cell Mol Med, 2005, 9(1): 59-71.
15. 　費洪榮, 王鳳澤. PI3K/Akt信號通路的調控與腫瘤血管生成[J]. 生命的化學, 2010, 30(1): 38-41.

《華西醫學》

磷脂酰肌醇3-激酶和磷酸化蛋白激酶B在膀胱尿路上皮癌中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《華西醫學》

磷脂酰肌醇3-激酶和磷酸化蛋白激酶B在膀胱尿路上皮癌中的表達及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料