外源性前列腺素E2對兔跟腱膠原含量的影響_《中國修復重建外科雜志》

作者：

李輝 , 唐康來 , 鄧銀栓 , 謝美明 , 常德海 , 陶旭 , 許建中

第三軍醫大學西南醫院骨科全軍矯形外科中心（重慶，400038）;

關鍵詞：

前列腺素E2 肌腱病膠原兔

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【摘要】目的 肌腱損傷后周圍前列腺素E2（prostaglandin E2，PGE2）及體外牽伸載荷條件下肌腱細胞產生的PGE2均升高，通過觀察外源性PGE2對兔跟腱膠原含量的影響，探討其與肌腱病發生的關系。方法取健康3～4月齡日本短耳兔24只，體重2.0～2.5 kg，雌雄不限；根據PGE2注射劑量不同隨機分為兩組（n=12）：低劑量組（50 ng）和高劑量組（500 ng）。隨機選擇一側后肢跟腱中部經皮注射0.2 mL對應劑量PGE2，對側對應部位注射0.2 mL生理鹽水作為對照，每周注射1次至處死。注射4、8周后兩組各處死6只實驗動物，大體觀察跟腱情況后，取雙側跟腱HE染色觀察細胞結構、基質形態，苦味酸-天狼星紅染色偏振光顯微鏡下觀察肌腱組織的膠原變化并定量分析Ⅰ、Ⅲ型膠原含量，透射電鏡觀測膠原纖維密度及直徑。結果 HE染色示兩組實驗側均出現膠原纖維結構破壞。苦味酸-天狼星紅染色示4、8周時兩組實驗側Ⅲ型膠原纖維均較對照側增加，Ⅰ型膠原減少（P lt; 0.05）；與低劑量組比較，高劑量組實驗側Ⅲ型膠原纖維增加，Ⅰ型膠原減少，Ⅲ/Ⅰ型膠原比值增高（P lt; 0.05）。透射電鏡示兩組4、8周時實驗側單位面積內總膠原纖維橫截面積所占百分比均較對照側降低，直徑 gt; 100 nm的纖維比例均較對照側降低，直徑 lt; 100 nm的纖維比例均增加（P lt; 0.05）；與低劑量組比較，高劑量組實驗側單位面積內總膠原纖維橫截面積所占百分比更低，直徑 gt; 100 nm的纖維比例均明顯降低，直徑 lt; 100 nm的比例明顯增加（P lt; 0.05）。結論重復注射PGE2能導致兔跟腱Ⅰ型膠原減少，Ⅲ型膠原增多，Ⅰ/Ⅲ型膠原比例倒置，單位面積總膠原纖維密度降低，直徑變細，可能與肌腱病發生相關。

引用本文： 李輝,唐康來,鄧銀栓,謝美明,常德海,陶旭,許建中. 外源性前列腺素E2對兔跟腱膠原含量的影響. 中國修復重建外科雜志, 2012, 26(3): 352-358. doi: 復制

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2.	Fredberg U, Stengaard-Pedersen K. Chronic tendinopathy tissue pathology, pain mechanisms, and etiology with a special focus on inflammation. Scand J Med Sci Sports, 2008, 18(1): 3-15.
3.	Fu SC, Rolf C, Cheuk YC, et al. Deciphering the pathogenesis of tendinopathy: a three-stages process. Sports Med Arthrosc Rehabil Ther Technol, 2010, 2: 30.
4.	Li Z, Yang G, Khan M, et al. Inflammatory response of human tendon fibroblasts to cyclic mechanical stretching. Am J Sports Med, 2004, 32(2): 435-440.
5.	Bosetti F, Langenbach R, Weerasinghe GR. Prostaglandin E2 and microsomal prostaglandin E synthase-2 expression are decreased in the cyclooxygenase-2-deficient mouse brain despite compensatory induction of cyclooxygenase-1 and Ca2+-dependent phospholipase A2. J Neurochem, 2004, 91(6): 1389-1397.
6.	Park JY, Pillinger MH, Abramson SB. Prostaglandin E2 synthesis and secretion: the role of PGE2 synthases. Clin Immunol, 2006, 119(3): 229-240.
7.	Yucel-Lindberg T, Olsson T, Kawakami T. Signal pathways involved in the regulation of prostaglandin E synthase-1 in human gingival fibroblasts. Cell Signal, 2006, 18(12): 2131-2142.
8.	Langberg H, Skovgaard D, Karamouzis M, et al. Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans. J Physiol, 1999, 515(Pt3): 919-927.
9.	Kjaer M, Langberg H, Heinemeier K, et al. From mechanical loading to collagen synthesis, structural changes and function in human tendon. Scand J Med Sci Sports, 2009, 19(4): 500-510.
10.	Yao L, Bestwick CS, Bestwick LA, et al. Phenotypic drift in human tenocyte culture. Tissue Eng, 2006, 12(7): 1843-1849.
11.	Khan MH, Li Z, Wang JH. Repeated Exposure of tendon to prostaglandin-E2 leads to localized tendon degeneration. Clin J Sport Med, 2005, 15(1): 27-33.
12.	Wang JH. Mechanobiology of tendon. J Biomech, 2006, 39(9): 1563-1582.
13.	Oliva F, Via AG, Maffulli N. Calcific tendinopathy of the rotator cuff tendones. Sports Med Arthrosc, 2011, 19(3): 237-243.
14.	Arnoczky SP, Lavagnino M, Egerbacher M, et al. Matrix metalloproteinase inhibitors prevent a decrease in the mechanical properties of stress-deprived tendons: an in vitro experimental study. Am J Sports Med, 2007, 35(5): 763-769.
15.	曹洪輝, 唐康來, 陳磊, 等. 新型肌腱細胞循環牽伸載荷系統的研制及運行效果觀察. 第三軍醫大學學報, 2010, 32(6): 507-510.
16.	鄧銀栓, 唐康來, 謝美明, 等. 不同牽伸載荷對體外培養人肌腱細胞纖維型肌動蛋白的影響. 中華醫學雜志, 2011, 91(25): 1780-1785.
17.	謝美明, 唐康來, 鄧銀栓, 等. 不同牽伸強度對體外人肌腱細胞磷脂酶A2/環氧合酶表達的影響. 中華創傷雜志, 2011, 27(9): 822-826.
18.	Lui PP, Chan LS, Lee YW, et al. Sustained expression of proteoglycans and collagen type III/type I ratio in a calcified tendinopathy model. Rheumatology (Oxford), 2010, 49(2): 231-239.
19.	Riley G. Tendinopathy—from basic science to treatment. Nat Clin Pract Rheumatol, 2008, 4(2): 82-89.
20.	O’Keeffe SA, Hogan BA, Eustace SJ, et al. Overuse injuries of the knee. Magn Reson Imaging Clin N Am, 2009, 17(4): 725-739.
21.	Pearce CJ, Ismail M, Calder JD. Is apoptosis the cause of noninsertional achilles tendinopathy? Am J Sports Med, 2009, 37(12): 2440-2444.

1. Walz DM, Newman JS, Konin GP, et al. Epicondylitis: pathogenesis, imaging, and treatment. Radiographics, 2010, 30(1): 167-184.
2. Fredberg U, Stengaard-Pedersen K. Chronic tendinopathy tissue pathology, pain mechanisms, and etiology with a special focus on inflammation. Scand J Med Sci Sports, 2008, 18(1): 3-15.
3. Fu SC, Rolf C, Cheuk YC, et al. Deciphering the pathogenesis of tendinopathy: a three-stages process. Sports Med Arthrosc Rehabil Ther Technol, 2010, 2: 30.
4. Li Z, Yang G, Khan M, et al. Inflammatory response of human tendon fibroblasts to cyclic mechanical stretching. Am J Sports Med, 2004, 32(2): 435-440.
5. Bosetti F, Langenbach R, Weerasinghe GR. Prostaglandin E2 and microsomal prostaglandin E synthase-2 expression are decreased in the cyclooxygenase-2-deficient mouse brain despite compensatory induction of cyclooxygenase-1 and Ca2+-dependent phospholipase A2. J Neurochem, 2004, 91(6): 1389-1397.
6. Park JY, Pillinger MH, Abramson SB. Prostaglandin E2 synthesis and secretion: the role of PGE2 synthases. Clin Immunol, 2006, 119(3): 229-240.
7. Yucel-Lindberg T, Olsson T, Kawakami T. Signal pathways involved in the regulation of prostaglandin E synthase-1 in human gingival fibroblasts. Cell Signal, 2006, 18(12): 2131-2142.
8. Langberg H, Skovgaard D, Karamouzis M, et al. Metabolism and inflammatory mediators in the peritendinous space measured by microdialysis during intermittent isometric exercise in humans. J Physiol, 1999, 515(Pt3): 919-927.
9. Kjaer M, Langberg H, Heinemeier K, et al. From mechanical loading to collagen synthesis, structural changes and function in human tendon. Scand J Med Sci Sports, 2009, 19(4): 500-510.
10. Yao L, Bestwick CS, Bestwick LA, et al. Phenotypic drift in human tenocyte culture. Tissue Eng, 2006, 12(7): 1843-1849.
11. Khan MH, Li Z, Wang JH. Repeated Exposure of tendon to prostaglandin-E2 leads to localized tendon degeneration. Clin J Sport Med, 2005, 15(1): 27-33.
12. Wang JH. Mechanobiology of tendon. J Biomech, 2006, 39(9): 1563-1582.
13. Oliva F, Via AG, Maffulli N. Calcific tendinopathy of the rotator cuff tendones. Sports Med Arthrosc, 2011, 19(3): 237-243.
14. Arnoczky SP, Lavagnino M, Egerbacher M, et al. Matrix metalloproteinase inhibitors prevent a decrease in the mechanical properties of stress-deprived tendons: an in vitro experimental study. Am J Sports Med, 2007, 35(5): 763-769.
15. 曹洪輝, 唐康來, 陳磊, 等. 新型肌腱細胞循環牽伸載荷系統的研制及運行效果觀察. 第三軍醫大學學報, 2010, 32(6): 507-510.
16. 鄧銀栓, 唐康來, 謝美明, 等. 不同牽伸載荷對體外培養人肌腱細胞纖維型肌動蛋白的影響. 中華醫學雜志, 2011, 91(25): 1780-1785.
17. 謝美明, 唐康來, 鄧銀栓, 等. 不同牽伸強度對體外人肌腱細胞磷脂酶A2/環氧合酶表達的影響. 中華創傷雜志, 2011, 27(9): 822-826.
18. Lui PP, Chan LS, Lee YW, et al. Sustained expression of proteoglycans and collagen type III/type I ratio in a calcified tendinopathy model. Rheumatology (Oxford), 2010, 49(2): 231-239.
19. Riley G. Tendinopathy—from basic science to treatment. Nat Clin Pract Rheumatol, 2008, 4(2): 82-89.
20. O’Keeffe SA, Hogan BA, Eustace SJ, et al. Overuse injuries of the knee. Magn Reson Imaging Clin N Am, 2009, 17(4): 725-739.
21. Pearce CJ, Ismail M, Calder JD. Is apoptosis the cause of noninsertional achilles tendinopathy? Am J Sports Med, 2009, 37(12): 2440-2444.

《中國修復重建外科雜志》

外源性前列腺素E2對兔跟腱膠原含量的影響

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《中國修復重建外科雜志》

外源性前列腺素E2對兔跟腱膠原含量的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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