脂肪干細胞及血管束植入法聯合應用對組織工程支架體內血管化的影響_《中國修復重建外科雜志》

作者：

姬文晨 , 楊佩 , 張越林 , 王春生 , 倪建龍 , 張永濤 , 王坤正

1 西安交通大學醫學院第二附屬醫院骨一科（西安，710004）;;
2 西安交通大學醫學院第三附屬醫院（陜西省人民醫院）神經外科;

關鍵詞：

組織工程支架脂肪干細胞血管束植入血管化大鼠

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目的探討脂肪干細胞（adipose-derived stem cells，ADSCs）及血管束植入法聯合應用對組織工程支
架體內血管化的影響，為臨床應用組織工程支架復合物治療股骨頭缺血性壞死提供理論依據。方法取4 月齡SD 大
鼠，分離培養ADSCs，并行成骨誘導鑒定。取第3 代ADSCs 接種于納米羥基磷灰石/ 聚酰胺66（nano-hydroxyapatide/
polyamide-66，nHA/PA66）支架上制備復合支架，掃描電鏡觀察細胞與支架復合情況。取4 月齡SD 大鼠24 只，體重
350 ～ 400 g，隨機分為3 組，每組8 只。A、B 組分離大鼠雙側腹壁下動、靜脈，分別將血管束植入培養10 d 的復合支架及
單純nHA/PA66 支架；C 組將培養10 d 的復合支架包埋入雙側股四頭肌中。術后2、4 周每組取4 只大鼠支架標本行HE
染色及CD34 免疫組織化學染色觀察，檢測其血管生成情況。結果所分離細胞經鑒定為ADSCs。掃描電鏡觀察示復
合培養10 d，細胞數目增多，形態完全伸展，呈長梭形。術后2、4 周，HE 染色及免疫組織化學染色均顯示A 組支架植入動、
靜脈周圍有大量血管生成，血管數量及管壁成熟度均優于同一時間點的B、C 組。術后2、4 周，A 組血管密度和血管直徑
均顯著大于B、C 組，B 組大于C 組，差異均有統計學意義（P lt; 0.05）。結論 ADSCs 和血管束植入法聯合應用可以促
進組織工程支架體內血管化程度。

引用本文： 姬文晨,楊佩,張越林,王春生,倪建龍,張永濤,王坤正. 脂肪干細胞及血管束植入法聯合應用對組織工程支架體內血管化的影響. 中國修復重建外科雜志, 2012, 26(2): 129-134. doi: 復制

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2.	Wen Q, Ma L, Chen YP, et al. Treatment of avascular necrosis of the femoral head by hepatocyte growth factor-transgenic bone marrow stromal stem cells. Gene Ther, 2008, 15(23): 1523-1535.
3.	Picinich SC, Mishra PJ, Mishra PJ, et al. The therapeutic potential of mesenchymal stem cell. Cell-tissue-based therapy. Expert Opin Biol Ther, 2007, 7(7): 965-973.
4.	Kalita SJ, Bhardwaj A, Bhatt HA. Nanocrystalline calcium phosphate ceramics in biomeical engineering. Materials Science and Engineering, 2007, 27(3): 441-449.
5.	趙文, 江昭林, 魏朝陽, 等. 人工椎板(納米羥基磷灰石/聚酰胺66)的臨床應用. 臨床骨科雜志, 2009, 12(4): 398-399.
6.	Cai X, Tong H, Shen X, et al. Preparation and characterization of homogeneous chitosan-polylactic acid/hydroxyapatite nanocomposite for bone tissue engineering and evaluation of its mechanical properties. Acta Biomater, 2009, 5(7): 2693-2703.
7.	戚孟春, 鄧久鵬, 董偉, 等. 骨髓間充質干細胞與支架材料nHA/PA66復合培養的生物活性研究. 口腔醫學研究, 2010, 26(6): 805-808.
8.	Doldercr JH, Abberton KM, Thompson EW, et al. Spontaneous large volume adipose tissue generation from a vascularized pedicled fat flap inside a chamber space. Tissue Eng, 2007, 13(4): 673-681.
9.	Pelissier P, Villars F, Mathoulin-Pelissier S, et al. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats. Plast Reconstr Surg, 2003, 111(6): 1932-1941.
10.	王簕, 裴國獻, 高梁斌, 等. 血管化組織工程骨修復兔股骨干骨缺損. 中華醫學雜志, 2010, 90(23): 1637-1641.
11.	王簕, 覃俊君, 裴國獻, 等. 血管束植入組織工程骨修復兔股骨缺損對血管內皮生長因子表達的影響. 中華創傷骨科雜志, 2009, 11(6): 540-545.
12.	Li B, Zeng Q, Wang H, et al. Effect of cytokines secreted by human adipose stromal cells on endothelial cells. J Huazhong Univ Sci Technolog Med Sci, 2006, 26(4): 396-398.
13.	Sadat S, Gehmert S, Song YH, et al. The cardioprotective effect of stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun, 2007, 363(3): 674-679.
14.	Shigemura N, Ukumura M, Mizuno S, et al. Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. Am J Transplant, 2006, 6(11): 2592-2600.
15.	Lu F, Mizuno H, Uysal CA, et al. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg, 2008, 121(1): 50-58.
16.	Suga H, Shigeura T, Matsumoto D, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy, 2007, 9(8): 738-745.
17.	De Francesco F, Tirino V, Desiderio V, et al. Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One, 2009, 4(8): e6537.
18.	Rehman J, Traktuev D, Li J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 2004, 109(10): 1292-1298.
19.	Sanz-Ruiz R, Santos ME, Muñoa MD, et al. Adipose tissue-derived stem cells: the friendly side of a classic cardiovascular foe. J Cardiovasc Transl Res, 2008, 1(1): 55-63.
20.	Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
21.	Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, et al. Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Cell Tissue Res, 2008, 332(3): 415-426.

1. Mont MA, Jones LC, Hungerford DS, et al. Nontraumatic osteonecrosis of the femoral head: ten years later. J Bone Joint Surg (Am), 2006, 88(5): 1117-1132.
2. Wen Q, Ma L, Chen YP, et al. Treatment of avascular necrosis of the femoral head by hepatocyte growth factor-transgenic bone marrow stromal stem cells. Gene Ther, 2008, 15(23): 1523-1535.
3. Picinich SC, Mishra PJ, Mishra PJ, et al. The therapeutic potential of mesenchymal stem cell. Cell-tissue-based therapy. Expert Opin Biol Ther, 2007, 7(7): 965-973.
4. Kalita SJ, Bhardwaj A, Bhatt HA. Nanocrystalline calcium phosphate ceramics in biomeical engineering. Materials Science and Engineering, 2007, 27(3): 441-449.
5. 趙文, 江昭林, 魏朝陽, 等. 人工椎板(納米羥基磷灰石/聚酰胺66)的臨床應用. 臨床骨科雜志, 2009, 12(4): 398-399.
6. Cai X, Tong H, Shen X, et al. Preparation and characterization of homogeneous chitosan-polylactic acid/hydroxyapatite nanocomposite for bone tissue engineering and evaluation of its mechanical properties. Acta Biomater, 2009, 5(7): 2693-2703.
7. 戚孟春, 鄧久鵬, 董偉, 等. 骨髓間充質干細胞與支架材料nHA/PA66復合培養的生物活性研究. 口腔醫學研究, 2010, 26(6): 805-808.
8. Doldercr JH, Abberton KM, Thompson EW, et al. Spontaneous large volume adipose tissue generation from a vascularized pedicled fat flap inside a chamber space. Tissue Eng, 2007, 13(4): 673-681.
9. Pelissier P, Villars F, Mathoulin-Pelissier S, et al. Influences of vascularization and osteogenic cells on heterotopic bone formation within a madreporic ceramic in rats. Plast Reconstr Surg, 2003, 111(6): 1932-1941.
10. 王簕, 裴國獻, 高梁斌, 等. 血管化組織工程骨修復兔股骨干骨缺損. 中華醫學雜志, 2010, 90(23): 1637-1641.
11. 王簕, 覃俊君, 裴國獻, 等. 血管束植入組織工程骨修復兔股骨缺損對血管內皮生長因子表達的影響. 中華創傷骨科雜志, 2009, 11(6): 540-545.
12. Li B, Zeng Q, Wang H, et al. Effect of cytokines secreted by human adipose stromal cells on endothelial cells. J Huazhong Univ Sci Technolog Med Sci, 2006, 26(4): 396-398.
13. Sadat S, Gehmert S, Song YH, et al. The cardioprotective effect of stem cells is mediated by IGF-I and VEGF. Biochem Biophys Res Commun, 2007, 363(3): 674-679.
14. Shigemura N, Ukumura M, Mizuno S, et al. Autologous transplantation of adipose tissue-derived stromal cells ameliorates pulmonary emphysema. Am J Transplant, 2006, 6(11): 2592-2600.
15. Lu F, Mizuno H, Uysal CA, et al. Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg, 2008, 121(1): 50-58.
16. Suga H, Shigeura T, Matsumoto D, et al. Rapid expansion of human adipose-derived stromal cells preserving multipotency. Cytotherapy, 2007, 9(8): 738-745.
17. De Francesco F, Tirino V, Desiderio V, et al. Human CD34+/CD90+ ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries. PLoS One, 2009, 4(8): e6537.
18. Rehman J, Traktuev D, Li J, et al. Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells. Circulation, 2004, 109(10): 1292-1298.
19. Sanz-Ruiz R, Santos ME, Muñoa MD, et al. Adipose tissue-derived stem cells: the friendly side of a classic cardiovascular foe. J Cardiovasc Transl Res, 2008, 1(1): 55-63.
20. Zuk PA, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228.
21. Jurgens WJ, Oedayrajsingh-Varma MJ, Helder MN, et al. Effect of tissue-harvesting site on yield of stem cells derived from adipose tissue: implications for cell-based therapies. Cell Tissue Res, 2008, 332(3): 415-426.

《中國修復重建外科雜志》

脂肪干細胞及血管束植入法聯合應用對組織工程支架體內血管化的影響

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《中國修復重建外科雜志》

脂肪干細胞及血管束植入法聯合應用對組織工程支架體內血管化的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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