成纖維細胞激活蛋白在小鼠肺纖維化模型中的表達_《中國呼吸與危重監護雜志》

作者：

魏麗 ^1# , 董麗 ¹ , 靳占峰 ^1,2 , 付志軍 ²

1 哈爾濱醫科大學病理學教研室( 黑龍江哈爾濱 150081) ;;
2 哈爾濱醫科大學法醫學教研室( 黑龍江哈爾濱 150081)通訊作者: 靳占峰, E-mail: jinzf2003@ yahoo. com. cn#現在工作單位: 鄭州市第三人民醫院;

關鍵詞：

成纖維細胞激活蛋白肺纖維化成纖維細胞轉化生長因子β1

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目的探討成纖維細胞激活蛋白( FAP) 在實驗性小鼠肺纖維化組織中的表達, 及其與α平滑肌肌動蛋白( α-SMA) 、轉化生長因子β1 ( TGF-β1 ) 之間的相互關系。方法氣管內滴入博萊霉素( 7 mg/kg) 制備肺纖維化模型, 采用免疫組化技術檢測小鼠肺纖維化組織中FAP、α-SMA 和TGF-β1的表達。結果正常肺組織無FAP表達; 纖維化組FAP 表達于細支氣管和大血管周圍的成纖維細胞, 成纖維細胞灶中FAP、α-SMA 和TGF-β1 表達部位相似, 但FAP 比α-SMA 的表達更廣泛, 比TGF-β1 的表達更強。FAP、α-SMA 和TGF-β1 與小鼠肺纖維化程度均呈正相關( r 值分別為0. 795、0. 766 和0. 628, P lt;0. 01) , 且FAP與TGF-β1 的表達呈正相關( r =0. 706, P lt; 0. 01) 。結論 FAP 特異性表達在小鼠肺纖維化組織的成纖維細胞灶中, 與肺纖維化程度有關。FAP 與TGF-β1 可能在肺纖維化形成中起協同作用。

引用本文： 魏麗 ,董麗,靳占峰,付志軍. 成纖維細胞激活蛋白在小鼠肺纖維化模型中的表達. 中國呼吸與危重監護雜志, 2010, 9(3): 301-305. doi: 復制

1.	Ramos C, Monta??o M, García-Alvarez J, et al. Fibroblasts fromidiopathic pulmonary fibrosis and normal lungs differ in growth rate,apoptosis, and tissue inhibitor of metalloproteinases expression. AmJRespir Cell Mol Biol, 2001, 24 : 591-598 .
2.	Flavell SJ, Hou TZ, Lax S, et al. Fibroblasts as novel therapeutictargets in chronic inflammation. Br J Pharmacol, 2008, 153: S241-S246.
3.	Wang XM, Yao TW, Nadvi NA, et al. Fibroblast activation proteinand chronic liver disease. Front Biosci, 2008, 13: 3168-3180.
4.	Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin-inducedinterstitial pulmonary disease in the nude, athymic mouse. Am RevRespir Dis, 1979, 120 : 893-899.
5.	Acharya PS, Zukas A, Chandan V, et al. Fibroblast activationprotein: a serine protease expressed at the remodeling interface in idiopathic pulmonary fibrosis. Hum Pathol, 2006, 37 : 352-360.
6.	Scotton CJ, Chambers RC. Molecular targets in pulmonary fibrosis:the myofibroblast in focus. Chest, 2007 , 132: 1311-1321.
7.	Wang XM, Yu DM, McCaughan GW, et al. Fibroblast activationprotein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line. Hepatology, 2005 , 42: 935-945.
8.	Artym VV, Kindzelskii AL, Chen WT, et al. Molecular proximity ofseprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton. Carcinogenesis, 2002, 23: 1593-1601.
9.	Chen WT. DPPIV and seprase in cancer invasion and angiogenesis.Adv Exp Med Biol, 2003 , 524: 197-203.
10.	Huang Y, Wang S, Kelly T. Seprase promotes rapid tumor growth and increased microvessel density in a mouse model of human breast cancer. Cancer Res, 2004 , 64: 2712-2716.
11.	Aimes RT, Zijlstra A, Hooper JD, et al. Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thromb Haemost, 2003, 89: 561-572 .
12.	Noble PW, Homer RJ. Idiopathic pulmonary fibrosis: new insights into pathogenesis. Clin Chest Med, 2004, 25: 749-758 .
13.	王興勝, 崔社懷, 楊雪梅, 等. 肺組織血管生成變化與肺纖維化關系探討. 中國呼吸與危重監護雜志, 2007, 6 : 378-380.
14.	White ES, Atrasz RG, Hu B, et al. Negative regulation of myofibroblast differentiation by PTEN ( Phosphatase and Tensin Homolog Deleted on chromosome 10 ) . Am J Respir Crit Care Med, 2006, 173: 112-121.
15.	Rettig WJ, Su SL, Fortunato SR, et al. Fibroblast activation protein: purification, epitope mapping and induction by growth factors. Int JCancer, 1994 , 58: 385 -392.
16.	Willis BC, duBois RM, Borok Z. Epithelial origin of myofibroblasts during fibrosis in the lung. Proc Am Thorac Soc, 2006, 3: 377-382.
17.	蔡琳, 吳壯, 徐軍. 博萊霉素誘導α平滑肌肌動蛋白Cre 重組酶轉基因小鼠肺纖維化上皮細胞-間質轉分化的研究. 中國呼吸與危重監護雜志, 2009, 8 , 52 -56.

1. Ramos C, Monta??o M, García-Alvarez J, et al. Fibroblasts fromidiopathic pulmonary fibrosis and normal lungs differ in growth rate,apoptosis, and tissue inhibitor of metalloproteinases expression. AmJRespir Cell Mol Biol, 2001, 24 : 591-598 .
2. Flavell SJ, Hou TZ, Lax S, et al. Fibroblasts as novel therapeutictargets in chronic inflammation. Br J Pharmacol, 2008, 153: S241-S246.
3. Wang XM, Yao TW, Nadvi NA, et al. Fibroblast activation proteinand chronic liver disease. Front Biosci, 2008, 13: 3168-3180.
4. Szapiel SV, Elson NA, Fulmer JD, et al. Bleomycin-inducedinterstitial pulmonary disease in the nude, athymic mouse. Am RevRespir Dis, 1979, 120 : 893-899.
5. Acharya PS, Zukas A, Chandan V, et al. Fibroblast activationprotein: a serine protease expressed at the remodeling interface in idiopathic pulmonary fibrosis. Hum Pathol, 2006, 37 : 352-360.
6. Scotton CJ, Chambers RC. Molecular targets in pulmonary fibrosis:the myofibroblast in focus. Chest, 2007 , 132: 1311-1321.
7. Wang XM, Yu DM, McCaughan GW, et al. Fibroblast activationprotein increases apoptosis, cell adhesion, and migration by the LX-2 human stellate cell line. Hepatology, 2005 , 42: 935-945.
8. Artym VV, Kindzelskii AL, Chen WT, et al. Molecular proximity ofseprase and the urokinase-type plasminogen activator receptor on malignant melanoma cell membranes: dependence on beta1 integrins and the cytoskeleton. Carcinogenesis, 2002, 23: 1593-1601.
9. Chen WT. DPPIV and seprase in cancer invasion and angiogenesis.Adv Exp Med Biol, 2003 , 524: 197-203.
10. Huang Y, Wang S, Kelly T. Seprase promotes rapid tumor growth and increased microvessel density in a mouse model of human breast cancer. Cancer Res, 2004 , 64: 2712-2716.
11. Aimes RT, Zijlstra A, Hooper JD, et al. Endothelial cell serine proteases expressed during vascular morphogenesis and angiogenesis. Thromb Haemost, 2003, 89: 561-572 .
12. Noble PW, Homer RJ. Idiopathic pulmonary fibrosis: new insights into pathogenesis. Clin Chest Med, 2004, 25: 749-758 .
13. 王興勝, 崔社懷, 楊雪梅, 等. 肺組織血管生成變化與肺纖維化關系探討. 中國呼吸與危重監護雜志, 2007, 6 : 378-380.
14. White ES, Atrasz RG, Hu B, et al. Negative regulation of myofibroblast differentiation by PTEN ( Phosphatase and Tensin Homolog Deleted on chromosome 10 ) . Am J Respir Crit Care Med, 2006, 173: 112-121.
15. Rettig WJ, Su SL, Fortunato SR, et al. Fibroblast activation protein: purification, epitope mapping and induction by growth factors. Int JCancer, 1994 , 58: 385 -392.
16. Willis BC, duBois RM, Borok Z. Epithelial origin of myofibroblasts during fibrosis in the lung. Proc Am Thorac Soc, 2006, 3: 377-382.
17. 蔡琳, 吳壯, 徐軍. 博萊霉素誘導α平滑肌肌動蛋白Cre 重組酶轉基因小鼠肺纖維化上皮細胞-間質轉分化的研究. 中國呼吸與危重監護雜志, 2009, 8 , 52 -56.

《中國呼吸與危重監護雜志》

成纖維細胞激活蛋白在小鼠肺纖維化模型中的表達

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《中國呼吸與危重監護雜志》

成纖維細胞激活蛋白在小鼠肺纖維化模型中的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料