吸煙對大鼠血清瘦素、脂聯素、IL-6 和C 反應蛋白的影響_《中國呼吸與危重監護雜志》

作者：

白學禮 , 許建英

山西醫科大學第一醫院呼吸科( 山西太原 030001)通訊作者: 許建英, E-mail: xujyty@ tom. com;

關鍵詞：

慢性阻塞性肺疾病吸煙瘦素脂聯素全身炎癥反應

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的通過研究不同吸煙量對大鼠血清瘦素、脂聯素、IL-6 及C 反應蛋白( CRP) 水平的影響, 探討瘦素和脂聯素等炎癥因子在吸煙所致COPD 全身炎癥反應中的作用。方法雄性Wistar 大鼠30 只, 隨機分為大量吸煙組, 小量吸煙組和對照組, 每組各10 只。大量吸煙組每次吸煙10 支, 每天吸煙2 次, 每周吸煙6 d, 共吸煙20 周; 小量吸煙組每次吸煙5 支, 吸煙時間同前。采用雙抗體夾心酶聯免疫吸附法( ABC-ELISA) 分別檢測血清瘦素、脂聯素、IL-6 及CRP 水平。結果大量吸煙組和小量吸煙組血清瘦素和脂聯素水平均較對照組降低( P 均 lt;0. 05) , 大量吸煙組與小量吸煙組差異無統計學意義( P gt;0. 05) 。大量吸煙組和小量吸煙組血清IL-6 和CRP 水平均較對照組升高( P 均 lt;0. 05) , 大量吸煙組較小量吸煙組水平高( P 均 lt;0. 05) 。結論吸煙可使大鼠血清瘦素與脂聯素水平下降, IL-6 與CRP水平升高, 提示瘦素、脂聯素等炎癥因子可能參與吸煙所致COPD 的全身炎癥反應。

引用本文： 白學禮,許建英. 吸煙對大鼠血清瘦素、脂聯素、IL-6 和C 反應蛋白的影響. 中國呼吸與危重監護雜志, 2010, 9(3): 298-300. doi: 復制

1.	van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
2.	Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
3.	Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
4.	Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
5.	許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復中表達的研究. 中華結核和呼吸雜志,1999, 22:417-419.
6.	Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
7.	Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
8.	Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
9.	Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
10.	Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
11.	Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
12.	Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
13.	Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
14.	Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
15.	Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.

1. van Weel C, Schellevis FG. Comorbidity and guidelines: conflicting interests. Lancet, 2006 , 367: 550-551.
2. Sin DD, Anthonisen NR, Soriano JB, et al. Mortality in COPD: Role of comorbidities. Eur Respir J, 2006, 28: 1245-1257.
3. Schols AM, Creutzerg EC, Buurman WA, et al. Plasma leptin is related to proinflammatory status and dietary intake in patients with chronic obstructive pulmonary disease. Am J Respir Crit Care Med,1999, 160: 1220-1226.
4. Kirdar S, Serter M, Ceylan E, et al. Adiponectin as a biomarker of systemic inflammatory response in smoker patients with stable and exacerbation phases of chronic obstructive pulmonary disease. Scand.J Clin Lab Invest, 2009, 69: 219 -224.
5. 許三林, 吳人亮, 陳春蓮, 等. 上皮鈣黏附素在吸煙小鼠呼吸道上皮損傷修復中表達的研究. 中華結核和呼吸雜志,1999, 22:417-419.
6. Chen H, Hansen MJ, Jones JE, et al. Long-term cigarette smoke exposure increases uncoupling protein expression but reduces energy intake. Brain Res, 2008, 1228: 81-88.
7. Hotta Y, Yatsuya H, Toyoshima H, et al. Low leptin but high insulin resistance of smokers in Japanese men. Diabetes Res Clin Pract,2008, 81: 358-364 .
8. Sull JW, Kim HJ, Yun JE, et al. Serumadiponectin is associated with smoking status in healthy Korean men. Endocr J, 2009, 56: 73-78.
9. Kirchgessner TG, Uysal KT, Wiesbrock SM, et al. Tumor necrosis factor-alpha contributes to obesity-related hyperleptinemia by regulating leptin release from adipocytes. J Clin Invest, 1997, 100 :2777-2782.
10. Trujillo ME, Sullivan S, Harten I, et al. Interleukin-6 regulates human adipose tissue lipid metabolism and leptin production in vitro. J Clin Endocrinol Metab, 2004, 89 : 5577 -5582 .
11. Fantuzzi G. Adipose tissue, adipokines, and inflammation. J Allergy Clin Immunol, 2005, 115: 911 -919.
12. Gettys TW, Harkness PJ, Waston PM. The β3-aderenergic receptor inhibits insulin-stinulated leptin secretion from isolated rat adipocytes. Endocrinology, 1996, 137: 4054-4057.
13. Bruun JM, Lihn AS, Verdich C, et al. Regulation of adiponectin by adipose tissue-derived cytokines: in vivo and in vitro investigations in humans. Am J Physiol Endocrinol Metab, 2003, 285 : E527-533.
14. Iwashima Y, Katauya T, Ishikawa K, et al. Association of hypoadiponectinemia with smoking habit in men. Hypertension,2005, 45: 1094-1100 .
15. Pepys MB, Hirschfield GM. C-reactive protein: a critical update. J Clin Invest, 2003 , 111: 1805-1812.

《中國呼吸與危重監護雜志》

吸煙對大鼠血清瘦素、脂聯素、IL-6 和C 反應蛋白的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國呼吸與危重監護雜志》

吸煙對大鼠血清瘦素、脂聯素、IL-6 和C 反應蛋白的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料