哮喘小鼠肺組織和肺T 細胞β1, 3-N-乙酰糖基轉移酶的表達_《中國呼吸與危重監護雜志》

作者：

張杰 , 郭雪君

上海交通大學醫學院附屬新華醫院呼吸內科( 上海 200092)通訊作者: 郭雪君, E-mail: guoxjz@ yahoo. com. cn#目前工作單位: 上海交通大學醫學院附屬第三人民醫院呼吸內科;

關鍵詞：

β1 3-N-乙酰糖基轉移酶哮喘小鼠 T 細胞肺

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目的研究支氣管哮喘模型小鼠肺組織及肺T 細胞中三種β1, 3-N-乙酰糖基轉移酶( Fringe) RFNG、LFNG、MFNG 的基因和蛋白表達的特點, 探討Fringe 是否與哮喘發病有關。方法使用卵白蛋白腹腔注射后霧化吸入法制備BALB/ c 小鼠哮喘模型, 并設置生理鹽水對照組。經Perco1 及尼龍毛法分離獲取肺T 細胞。采用半定量RT-PCR 檢測兩組小鼠肺組織及肺T 細胞中RFNG、LFNG、MFNG mRNA 表達;Western blot 檢測兩組小鼠肺組織中RFNG、LFNG、MFNG 蛋白表達。結果兩組小鼠肺組織及肺T 細胞中均存在三種Fringe 表達。哮喘組肺組織及肺T 細胞的RFNGmRNA 表達較對照組增加( 肺組織: 0. 92 ±0. 35 比0. 51 ±0. 13, P lt;0. 01; 肺T 細胞: 0. 33 ±0. 06 比0. 18 ±0. 07, P lt; 0. 01) ; LFNG mRNA 表達較對照組減少( 肺組織: 0. 77 ±0. 32 比1. 61 ±0. 31, P lt;0. 01; 肺T細胞: 0. 49 ±0. 19 比0. 71 ±0. 03, P lt;0. 01) 。MFNG 在肺組織中的表達無明顯差異( 肺組織: 1. 44 ±0. 29 比1. 70 ±0. 44, P gt; 0. 05) , 但MFNG 在肺T 細胞中的表達明顯減少( 0. 11 ±0. 03 比0. 52 ±0. 18, P lt; 0. 01) 。肺組織中三種Fringe 蛋白表達與肺組織的mRNA 結果相似。哮喘組的RFNG 蛋白表達高于對照組( 1. 17 ±0. 04 比0. 68 ±0. 07, P lt; 0. 05) , MFNG 蛋白表達在兩者之間沒有明顯的差異( 8. 10 ±0. 60 比9. 12 ±0. 07, P gt; 0. 05) , 而LFNG 的蛋白表達則低于對照組( 4. 11 ±0. 38比6. 41 ±0. 11, P lt;0. 05) 。結論三種Fringe 的異常表達可能與支氣管哮喘的發病有關。

引用本文： 張杰,郭雪君. 哮喘小鼠肺組織和肺T 細胞β1, 3-N-乙酰糖基轉移酶的表達. 中國呼吸與危重監護雜志, 2010, 9(2): 128-132. doi: 復制

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2.	Gerard FH.Notch signaling in the immune system.J Leukoc Biol,2003,74:971-981.
3.	Okajima T,Xu A,Irvine KD.Modulation of Notch-Ligand Binding by Protein O-Fucosyltransferase 1 and Fringe.J Biol Chem,2003;278:42340-42345.
4.	Moloney DJ,Shair LH,Lu FM,et al.Mammalian Notch1 Is Modified with Two Unusual Forms of O-Linked Glycosylation Found on Epidermal Growth Factor-like Modules.J Biol Chem,2000,275:9604-9611.
5.	Lei L,Xu A,Panin VM,et al.An O-fucose site in the ligand binding domain inhibits Notch activation,2003,130:6411-6421.
6.	Xu A,Haines N,Dlugosz M.In Vitro Reconstitution of the Modulation of Drosophila Notch-Ligand Binding by Fringe.J Biol Chem,2007,282:35153-35162.
7.	Yang LT,Nichols JT,Yao C,et al.Fringe Glycosyltransferases Differentially Modulate Notch1 Proteolysis Induced by Delta1 and Jagged1.Mol Biol Cell,2005,16:927-942.
8.	劉春濤,王曾禮,馮玉麟,等.哮喘動物模型氣道中白細胞介素5和10的動態變化及其意義.中華結核與呼吸雜志,2000;23:239-242.
9.	周敏,郭雪君,楊敏.支氣管哮喘BALB/c小鼠肺組織及肺T細胞中Notch1的表達.上海交通大學學報（醫學版）,2007,27:161-164.
10.	楊敏,郭雪君,徐衛國等.Notch配體在哮喘小鼠肺中的表達.中國呼吸與危重監護雜志,2007;6:257-230.
11.	O′Byrne PM.Cytokines or Their Antagonists for the Treatment of Asthma.Chest,2006,130:244-250.
12.	Pernis AB,Rothman PB.JAK-STAT signaling in asthma.J Clin Invest.2002,109:1279-1283.
13.	Kheradmand F,Kiss A,Xu J,et al.A Protease-Activated Pathway Underlying Th Cell Type 2 Activation and Allergic Lung Disease1.J Immunol,2002,169:5904-5911.
14.	Georas SN,Guo J,De Fanis U,et al.T-helper cell type-2 regulation in allergic disease.Eur Respir J,2005,26:1119-1137.
15.	Gerard F.Hoyne Notch signaling in the immune system J.Leukoc.Biol.,Dec 2003,74:971.
16.	Sun-Ok Yoon,Xin Zhang,Paul Berner,Bianca Blom,et al.Notch Ligands Expressed by Follicular Dendritic Cells Protect Germinal Center B Cells from Apoptosis J.Immunol.,Jul 2009,183:352 - 358.
17.	Jonathon F.Hutton,Tessa Gargett,Timothy J.Sadlon,et al.Development of CD4+CD25+FoxP3+ regulatory T cells from cord blood hematopoietic progenitor cells J.Leukoc.Biol.,Mar 2009,85:445 - 451.
18.	Amsen D,Blander JM,Lee G,et a1.Instruction of Distinct CD4 T Helper Cell Fates by Different Notch Ligands on Antigen-Presenting Cells.Cell,2004,ll7:515-526.
19.	Amsen D,Antov A,Jankovic D,et al.Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch.Immunity,Jul 2007；27（1）:89-99.
20.	Hozumi K,Abe N,Chiba S,et a1.Active form of notch member can enforce T lymphopoiesis on lymphoid progenitors in the monolayer culture specific for B cell development.J Immunol,2003,170:4973-4979.
21.	Maekawa Y,Tsukumo S,Chiba H,et a1.Delta1-Notch3 Interactions Bias the Functional Differentiation of Activated CD4+ T Cells.Immunity,2003,19:549-559.
22.	Shi S,Stanley P.Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways.PNAS.2003,100:5234-5239.
23.	Okajima T,Irvine KD.Regulation of Notch Signaling by O-Linked Fucose.Cell,2002,111:893-904.
24.	Ge CH,Stanley P.The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development.PNAS,2008,105:1539-1544.
25.	Liangtung Y,James TN,Christine Y,et a1.Fringe Glycosyltransferases Differentially Modulate Notch1 Proteolysis Induced by Delta1 and Jagged1.Mol Biol Cell,2005,16:927-942.

1. Guo XJ,Zhou M,Ren LP,et al.Small interfering RNA mediated knockdown of Notch1 in lung T cells of asthmatic mouse affects T cells differentiation.Chin Med J 2009,122:2647-2651.
2. Gerard FH.Notch signaling in the immune system.J Leukoc Biol,2003,74:971-981.
3. Okajima T,Xu A,Irvine KD.Modulation of Notch-Ligand Binding by Protein O-Fucosyltransferase 1 and Fringe.J Biol Chem,2003;278:42340-42345.
4. Moloney DJ,Shair LH,Lu FM,et al.Mammalian Notch1 Is Modified with Two Unusual Forms of O-Linked Glycosylation Found on Epidermal Growth Factor-like Modules.J Biol Chem,2000,275:9604-9611.
5. Lei L,Xu A,Panin VM,et al.An O-fucose site in the ligand binding domain inhibits Notch activation,2003,130:6411-6421.
6. Xu A,Haines N,Dlugosz M.In Vitro Reconstitution of the Modulation of Drosophila Notch-Ligand Binding by Fringe.J Biol Chem,2007,282:35153-35162.
7. Yang LT,Nichols JT,Yao C,et al.Fringe Glycosyltransferases Differentially Modulate Notch1 Proteolysis Induced by Delta1 and Jagged1.Mol Biol Cell,2005,16:927-942.
8. 劉春濤,王曾禮,馮玉麟,等.哮喘動物模型氣道中白細胞介素5和10的動態變化及其意義.中華結核與呼吸雜志,2000;23:239-242.
9. 周敏,郭雪君,楊敏.支氣管哮喘BALB/c小鼠肺組織及肺T細胞中Notch1的表達.上海交通大學學報（醫學版）,2007,27:161-164.
10. 楊敏,郭雪君,徐衛國等.Notch配體在哮喘小鼠肺中的表達.中國呼吸與危重監護雜志,2007;6:257-230.
11. O′Byrne PM.Cytokines or Their Antagonists for the Treatment of Asthma.Chest,2006,130:244-250.
12. Pernis AB,Rothman PB.JAK-STAT signaling in asthma.J Clin Invest.2002,109:1279-1283.
13. Kheradmand F,Kiss A,Xu J,et al.A Protease-Activated Pathway Underlying Th Cell Type 2 Activation and Allergic Lung Disease1.J Immunol,2002,169:5904-5911.
14. Georas SN,Guo J,De Fanis U,et al.T-helper cell type-2 regulation in allergic disease.Eur Respir J,2005,26:1119-1137.
15. Gerard F.Hoyne Notch signaling in the immune system J.Leukoc.Biol.,Dec 2003,74:971.
16. Sun-Ok Yoon,Xin Zhang,Paul Berner,Bianca Blom,et al.Notch Ligands Expressed by Follicular Dendritic Cells Protect Germinal Center B Cells from Apoptosis J.Immunol.,Jul 2009,183:352 - 358.
17. Jonathon F.Hutton,Tessa Gargett,Timothy J.Sadlon,et al.Development of CD4+CD25+FoxP3+ regulatory T cells from cord blood hematopoietic progenitor cells J.Leukoc.Biol.,Mar 2009,85:445 - 451.
18. Amsen D,Blander JM,Lee G,et a1.Instruction of Distinct CD4 T Helper Cell Fates by Different Notch Ligands on Antigen-Presenting Cells.Cell,2004,ll7:515-526.
19. Amsen D,Antov A,Jankovic D,et al.Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch.Immunity,Jul 2007；27（1）:89-99.
20. Hozumi K,Abe N,Chiba S,et a1.Active form of notch member can enforce T lymphopoiesis on lymphoid progenitors in the monolayer culture specific for B cell development.J Immunol,2003,170:4973-4979.
21. Maekawa Y,Tsukumo S,Chiba H,et a1.Delta1-Notch3 Interactions Bias the Functional Differentiation of Activated CD4+ T Cells.Immunity,2003,19:549-559.
22. Shi S,Stanley P.Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways.PNAS.2003,100:5234-5239.
23. Okajima T,Irvine KD.Regulation of Notch Signaling by O-Linked Fucose.Cell,2002,111:893-904.
24. Ge CH,Stanley P.The O-fucose glycan in the ligand-binding domain of Notch1 regulates embryogenesis and T cell development.PNAS,2008,105:1539-1544.
25. Liangtung Y,James TN,Christine Y,et a1.Fringe Glycosyltransferases Differentially Modulate Notch1 Proteolysis Induced by Delta1 and Jagged1.Mol Biol Cell,2005,16:927-942.

《中國呼吸與危重監護雜志》

哮喘小鼠肺組織和肺T 細胞β1, 3-N-乙酰糖基轉移酶的表達

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《中國呼吸與危重監護雜志》

哮喘小鼠肺組織和肺T 細胞β1, 3-N-乙酰糖基轉移酶的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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