CCL1/CCR8在哮喘小鼠肺組織中的表達及地塞米松對其作用的研究_《中國呼吸與危重監護雜志》

作者：

王澤宇 ,  劉劍波

鄭州大學第二附屬醫院呼吸內科（河南鄭州 450014）;

關鍵詞：

哮喘 CC類趨化因子配體1 CC 類趨化因子受體8 白細胞介素4 地塞米松

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目的觀察哮喘小鼠肺組織CCL1 /CCR8 mRNA 的表達及糖皮質激素對其表達的影響, 探討CCL1 /CCR8 在哮喘發病機制中的作用。方法 30 只小鼠隨機分為對照組、哮喘組和地塞米松組, 每組10 只。哮喘組和地塞米松組用卵白蛋白致敏激發建立哮喘模型, 地塞米松組給予腹腔注射地塞米松( 2 mg/kg) , 對照組均以生理鹽水替代。測定BALF 中細胞總數和分類計數, 采用酶聯免疫法檢測BALF 中IL-4 水平, RT-PCR 檢測肺組織CCR8 mRNA 和CCL1 mRNA 的表達。結果對照組、哮喘組和地塞米松組BALF 中嗜酸粒細胞百分比[ ( 0. 6 ±0. 1) % 、( 32. 4 ±3. 4) % 和( 8.9 ±0. 9) % , P lt;0. 01] 、淋巴細胞百分比[ ( 2.9 ±0. 5) % 、( 14. 0 ±3. 0) % 和( 6. 3 ±0. 6) % , P lt;0. 01] 、IL-4濃度[ ( 5.16 ±1. 23) pg /mL、( 47. 67 ±11. 67) pg/mL 和( 19. 76 ±4. 72) pg/mL, P lt;0. 01] 差異均有統計學意義, 其中哮喘組較對照組顯著增高( P lt;0. 01) , 地塞米松組較哮喘組顯著降低但仍顯著高于對照組( P lt;0. 01) 。對照組、哮喘組和地塞米松組CCL1 mRNA 表達( 0.11 ±0. 06、0. 76 ±0. 16 和0. 53 ±0. 13, P lt;0. 01) 、CCR8 mRNA 表達( 0. 18 ±0. 09、1. 26 ±0. 13 和0. 79 ±0. 12, P lt; 0. 01) 差異均有統計學意義, 其中哮喘組較對照組顯著增高( P lt;0. 01) , 地塞米松組較哮喘組顯著降低但仍顯著高于對照組( P lt;0. 01) 。結論CCL1/CCR8 在哮喘小鼠肺組織中的基因表達增強。糖皮質激素可能通過減少CCL1 /CCR8 的基因表達, 減輕哮喘氣道炎癥。

引用本文： 王澤宇,劉劍波. CCL1/CCR8在哮喘小鼠肺組織中的表達及地塞米松對其作用的研究. 中國呼吸與危重監護雜志, 2010, 9(1): 32-35. doi: 復制

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4.	Cantor J, Haskins K. Recruitment and activation of macrophages by pathogenic CD4 T cells in type 1 diabetes: evidence for involvement of CCR8 and CCL1.J Immunol,2007,79:5760-5767.
5.	Larché M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol,2003,111:450-463.
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7.	Moser B, Loetscher M,Piali L,et al.Lymphocyte responses to chemokines.Int Rev Immunol, 1998,16:323-344.
8.	Gonzalo JA,Qiu Y,Lora JM,et al.Coordinated involvement of mast cells and T cells in allergic mucosal inflammation:critical role of the CC chemokine ligand 1:CCR8 axis.J Immunol, 2007,179:1740-1750.
9.	Chensue SW,Lukacs NW,Yang TY,et al.Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J Exp Med, 2001,193:573-584.
10.	Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
11.	Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
12.	李鋒,劉榮玉.氣道炎癥和重構在小鼠哮喘模型中的變化及地塞米松的干預作用。中國呼吸與危重監護雜志.2005,4:110-114.

1. Heijink IH,Van Oosterhout AJ.Targeting T cells for asthma.Curr Opin Pharmacol,2005 ,5:227-231.
2. Zingoni A,Soto H,Hedrick JA,et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells.J Immunol,1998,161:547-551.
3. Gombert M, Dieu-Nosjean MC, Winterberg F,et al. CCL1-CCR8 interactions: an axis mediating the recruitment of T cells and Langerhans-type dendritic cells to sites of atopic skin inflammation. J Immunol, 2005,174:5082-5091.
4. Cantor J, Haskins K. Recruitment and activation of macrophages by pathogenic CD4 T cells in type 1 diabetes: evidence for involvement of CCR8 and CCL1.J Immunol,2007,79:5760-5767.
5. Larché M, Robinson DS, Kay AB. The role of T lymphocytes in the pathogenesis of asthma. J Allergy Clin Immunol,2003,111:450-463.
6. Laudanna C, Kim JY, Constantin G,et al. Rapid leukocyte integrin activation by chemokines. Immunol Rev,2002,186:37-46.
7. Moser B, Loetscher M,Piali L,et al.Lymphocyte responses to chemokines.Int Rev Immunol, 1998,16:323-344.
8. Gonzalo JA,Qiu Y,Lora JM,et al.Coordinated involvement of mast cells and T cells in allergic mucosal inflammation:critical role of the CC chemokine ligand 1:CCR8 axis.J Immunol, 2007,179:1740-1750.
9. Chensue SW,Lukacs NW,Yang TY,et al.Aberrant in vivo T helper type 2 cell response and impaired eosinophil recruitment in CC chemokine receptor 8 knockout mice. J Exp Med, 2001,193:573-584.
10. Chung CD, Kuo F, Kumer J,et al. CCR8 is not essential for the development of inflammation in a mouse model of allergic airway disease. J Immunol,2003,170:581-587.
11. Hoshino M,Nakagawa T,Sano Y,et al.Effect of inhaled corticosteroid on an immunoreactive thymus and activation-regulated chemokine expression in the bronchial biopsies from asthmatics. Allergy,2005,60:317-322.
12. 李鋒,劉榮玉.氣道炎癥和重構在小鼠哮喘模型中的變化及地塞米松的干預作用。中國呼吸與危重監護雜志.2005,4:110-114.

《中國呼吸與危重監護雜志》

CCL1/CCR8在哮喘小鼠肺組織中的表達及地塞米松對其作用的研究

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《中國呼吸與危重監護雜志》

CCL1/CCR8在哮喘小鼠肺組織中的表達及地塞米松對其作用的研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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