水通道蛋白1 與胸腔積液發生機制的體內研究_《中國呼吸與危重監護雜志》

作者：

廖槐 ,  謝燦茂

中山大學附屬第一醫院呼吸內科（廣東廣州 510080）;

關鍵詞：

水通道蛋白1 胸膜胸腔積液地塞米松內毒素紅霉素高滲鹽水

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目的探討水通道蛋白1( AQP1 ) 在胸腔積液發生機制中的作用。方法 SD 大鼠按實驗設計分為5 組( n =24) : 地塞米松組、內毒素組、紅霉素組、高滲鹽水組及對照組, 通過免疫組織化學染色測定AQP1 在胸膜上的表達和定位; 采用Real-time RT-PCR 方法對不同刺激因子作用下不同時間胸膜組織AQP1 的基因表達進行定量分析。結果大鼠臟、壁層胸膜間皮細胞及壁層胸膜下毛細血管內皮上均存在AQP1 的表達, 臟層胸膜間皮細胞上AQP1 的表達較壁層胸膜明顯[ ( 7. 43 ±2. 02) ×104 copy / μg 比( 4. 14 ±1. 12) ×104 copy/ μg, P lt;0. 05] 。地塞米松、高滲鹽水上調大鼠胸膜組織AQP1 的表達( P lt;0. 05) ; 紅霉素、內毒素下調胸膜組織上AQP1 的表達( P lt;0. 05) 。結論地塞米松、內毒素、紅霉素和高滲鹽水刺激下胸膜組織AQP1 表達的改變, 提示存在炎癥因子和滲透梯度下調控AQP1 的途徑, 胸膜組織上AQP1 可能參與了炎癥性胸腔積液的分泌和吸收。

引用本文： 廖槐,謝燦茂. 水通道蛋白1 與胸腔積液發生機制的體內研究. 中國呼吸與危重監護雜志, 2009, 09(2): 151-155. doi: 復制

1.	Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
2.	Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
3.	Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
4.	King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
5.	Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
6.	張偉, 謝燦茂, 黃建強, 等. 高滲液對大鼠胸膜間皮細胞水通道蛋白1 表達的影響. 中華醫學雜志, 2007, 87: 2508-2511.
7.	張偉, 謝燦茂. 角叉菜膠致炎癥性胸腔積液大鼠胸膜上水通道蛋白1 的表達. 中國呼吸與危重監護雜志, 2008, 7: 190-194.
8.	Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
9.	Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
10.	Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
11.	Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
12.	黃建強, 謝燦茂. 紅霉素對人胸膜間皮細胞生長及腫瘤壞死因子-α、轉化生長因子-β1 分泌和縫隙連接蛋白43 表達的影響.中華結核和呼吸雜志, 2003, 26: 206-209.
13.	Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
14.	Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
15.	Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
16.	Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
17.	Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
18.	Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
19.	Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
20.	Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
21.	Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
22.	Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
23.	Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
24.	謝艷萍, 陳才平, 王建春, 等. 脂多糖、腫瘤壞死因子-α、白細胞介素-1β鼠肺微血管內皮細胞水通道蛋白-1 表達的影響. 中國呼吸與危重監護雜志, 2005, 4: 142-145.

1. Preston GM, Agre P. Isolation of the cDNA for erythrocytes integral membrane protein of 28 kD:Member of an ancient channel family.Proc Natl Acad Sci USA, 1991, 88: 11110-11114.
2. Verkman AS. Physiological importance of aquaporin water channels.Ann Med, 2002, 34: 192-200.
3. Agre P. The aquaporin water channels. Proc Am Soc, 2006, 3: 5-13.
4. King LS, Nielsen S, Agre P. Respiratory Aquaporins in Lung Inflammation. Am J Respir Cell Mol Biol, 2000 , 22: 8 -10 .
5. Towne EE, Harrod KS. Decreased Expression of Aquaporin( AQP) 1 and AQP5 in Mouse Lung after Acute Viral Infection. Am J Respir Cell Mol Biol, 2000, 22: 34-44.
6. 張偉, 謝燦茂, 黃建強, 等. 高滲液對大鼠胸膜間皮細胞水通道蛋白1 表達的影響. 中華醫學雜志, 2007, 87: 2508-2511.
7. 張偉, 謝燦茂. 角叉菜膠致炎癥性胸腔積液大鼠胸膜上水通道蛋白1 的表達. 中國呼吸與危重監護雜志, 2008, 7: 190-194.
8. Yang B, Folkesson HG, Yang J, et al. Reduced osmotic water permeability of the peritoneal barrier in aquaporin-1 knockout mice.Am J Physiol Cell Physiol, 1999, 276: c76-c81.
9. Moon C, King LS, Agre P. AQP1 expression in erythroleukemia cells: genetic regulation of glucocorticoid and chemical induction.Am J Physiol Cell Physiol, 1997, 273: c1562-c1570.
10. Jiao G, Li E, Yu R. Decreased expression of AQP1 and AQP5 in acute injured lungs in rats. Chin Med J, 2002, 115: 963-967.
11. Song Y, Fukuda N, Bai C, et al. Role of aquaporins in alveolar fluid clearance in neonatal and adult lung, and in oedema formation following acute lung injury: studies in transgenic aquaporin null mice. J Physiol, 2000, 525: 771-779.
12. 黃建強, 謝燦茂. 紅霉素對人胸膜間皮細胞生長及腫瘤壞死因子-α、轉化生長因子-β1 分泌和縫隙連接蛋白43 表達的影響.中華結核和呼吸雜志, 2003, 26: 206-209.
13. Miserocchi G. Physiology and pathophysiology of pleural fluid turnover. Eur Respir J, 1997, 10: 219-225.
14. Miserocchi G, Venturoli D, Negrini D, et al.Model of pleural fluid turnover. J Appl Physiol, 1993, 75: 1798-1806.
15. Wiener-Kronish JP, Albertine KH, Lick V, et al. Protein egress and entry rates in pleural fluid and plasma in sheep. J Appl Physiol, 1984, 56: 459-463.
16. Lee CH, Wang WJ, Lan RS, et al. Corticosteroids in the treatment of tuberculous pleurisy. A double-blind, placebo-controlled,randomized study. Chest, 1988, 94: 256-1259.
17. Xie CM, Teixeira LR, McGovern JP, et al. Systemic corticosteroids decrease the effectiveness of talc pleurodesis. AmJ Respir Crit Care Med, 1998, 157: 1441-1444.
18. Gopi A, Madhavan SM, Sharma SK, et al. Diagnosis and treatment of tuberculous pleural effusion in 2006. Chest, 2007, 131: 880-889.
19. Sartori C, Matthay MA. Alveolar epithelial fluid transport in acute lung injury: new insights. Eur Respir J, 2002, 20: 1299-1313.
20. Leitch V, Agre P, King LS. Altered ubiquitination and stability of aquaporin-1 in hypertonic stress. PNAS, 2001, 98: 2894-2898.
21. Hoffert JD, Agre P, King LS. Hypertonic Induction of Aquaporin-5 Expression through an ERK-dependent Pathway. J Biol Chem,2000, 275: 9070-9077.
22. Song Y, Matthay MA, Verkman AS. Role of Aquaporin water channels in Pleural fluid dynamics. Am J Physiol Cell Physiol,2000, 279: 1744-1750.
23. Fang W, Qian J, Yu Z, et al. Expression of aquaporin-1 in the human peritoneum and the effect of peritoneal dialysis on its expression. Chin Med J, 2003, 116: 1370-1373.
24. 謝艷萍, 陳才平, 王建春, 等. 脂多糖、腫瘤壞死因子-α、白細胞介素-1β鼠肺微血管內皮細胞水通道蛋白-1 表達的影響. 中國呼吸與危重監護雜志, 2005, 4: 142-145.

《中國呼吸與危重監護雜志》

水通道蛋白1 與胸腔積液發生機制的體內研究

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《中國呼吸與危重監護雜志》

水通道蛋白1 與胸腔積液發生機制的體內研究

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