免疫毒素的制備及特異性淋巴細胞無反應性的誘導_《中國普外基礎與臨床雜志》

作者：

蘭平 , 劉嘉林 , 嚴律南 , 肖路加

華西醫科大學附屬第一醫院外科(成都610041);

關鍵詞：

CD4 CD8 免疫毒素免疫無反應性

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為減損排斥反應性淋巴細胞克隆，采用異型雙功能試劑SPDP、2-IT連接antiCD4、CD8單克隆抗體與天花粉毒素(TCS)，應用Sephacryl S-200分離游離TCS，采用SDS-PAGE電泳及細胞毒性分析測定合成的免疫毒素的純度和生物學活性。電泳結果顯示，連接混合物中含有連接的免疫毒素、游離的單克隆抗體和TCS，Sephacryl S-200可除去游離的TCS。體外，免疫毒素可特異性殺傷大鼠脾細胞，其作用具有劑量依賴性。由此提示抗CD4、CD8免疫毒素可以誘導免疫無反應性。

引用本文： 蘭平,劉嘉林,嚴律南,肖路加. 免疫毒素的制備及特異性淋巴細胞無反應性的誘導. 中國普外基礎與臨床雜志, 1998, 5(6): 321-323. doi: 復制

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2. Cobbold SP, Adams E, Marshall SE, et al. Mechanisms of peripheral tolerance and suppression induced by monoclonal antibodies to CD4 and CD8. Immunol Rev, 1996; 149∶5.
3. Carlsson J, Drevin H, Axen R. Protein thilolation and raversible proteinprotein conjugation. Nsuxxinimidy13(2pyridyldithio) propionate, a new heterobifunctional reagent. Biochem J, 1978; 173(3)∶723.
4. Wang QC. TrichosanthinmAb conjugate sepcifically cytotoxic to human hepatoma cell in vitro. Cancer Res, 1991; 51(12)∶3353.
5. Ellman GL. Tissue sulfhgdryl groups. Arch Biochem Biophy, 1959; 82(1)∶70.
6. Kagi D, Vignaux F, Ledermann B, et al. Fas and perforin pathways as major mechanisms of T cellmediated cytotoxicity. Science, 1994; 265(5171)∶528.
7. Stalder T, Hahn S, Erb P. Fas antigen is a major targed molecule for CD4＋T cell mediated cytotoxicity. J Immunol, 1994; 152(3)∶1127.
8. Smith CV, Sablinski T, Arn JS, et al. In vivo treatment with monoclonal antibodies directed against CD4 and CD8 antigens in miniature swine. J Immunother Emphasis Tumor Immunol, 1994; 16(2)∶105.
9. Kochi SH, Collier RJ. DNA fragmentation and cytolysis in V937 cell treatment with diphtheria toxin or other inhibitors of protein synthesis. Exp Cell Res, 1993; 208(1)∶296.
10. Waddick KG, Myers DE, Gunther R, et al. In vivo antileukemic activity of B43pokweed antiviral protein against radiation resistant human Bcell procursor leukemia cells. Blood, 1996; 186(11)∶4228.

《中國普外基礎與臨床雜志》

免疫毒素的制備及特異性淋巴細胞無反應性的誘導

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《中國普外基礎與臨床雜志》

免疫毒素的制備及特異性淋巴細胞無反應性的誘導

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料