胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產生及意義_《中國普外基礎與臨床雜志》

作者：

高軍 ¹ , 張淑文 ² , 李非 ²

1.北京市第六醫院外科（北京100007）;;
2.北京市宣武醫院普外科（北京100053）;

關鍵詞：

胰蛋白酶原激活肽急性胰腺炎蛋白酶抑制劑

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目的探討大鼠實驗性急性胰腺炎（AP）模型中胰蛋白酶原激活肽（TAP）的產生及意義。方法將90只SD大鼠隨機分為5組： EP組（3%牛磺膽酸鈉逆行膽胰管注射組）； NP組（5%牛磺膽酸鈉逆行膽胰管注射組）； TP組（3%牛磺膽酸鈉逆行膽胰管注射后半小時經股靜脈注入烏司他丁組）； CP組（0.9%生理鹽水逆行膽胰管注射組）； OP組（假手術組）。分別于制模后3、6及24小時后處死動物，取血測定血淀粉酶和TAP水平，同時取胰腺標本觀察其組織病理學改變并評分。結果 NP組胰腺病變明顯重于EP組。制模后3小時和6小時血漿TAP水平NP組分別為（4.798±0.169）nmol/L和(3.999±0.299)nmol/L，明顯高于EP組的（2.416±0.148）nmol/L和(3.356±0.211)nmol/L；在制模6小時后TP組血漿TAP水平為（1.611±0.113）nmol/L，比EP組的(3.356±0.211)nmol/L明顯降低。EP組與NP組血漿TAP水平差異的出現早于兩組間胰腺組織病理學改變差異的出現。結論血漿TAP水平與大鼠實驗性AP的嚴重程度有關。血漿TAP水平可以作為早期預測實驗性AP嚴重程度的指標。

引用本文： 高軍,張淑文,李非. 胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產生及意義. 中國普外基礎與臨床雜志, 2001, 8(5): 301-303下轉306. doi: 復制

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1. Gudmundsdottir A,Gudmundsdottir E,Oskarsson S,et al. Isolation and characterization of cDNAs from Atlantic cod encoding two different forms of trypsinogen 〔Ｊ〕. Eur J Biochem,1993; 217(3)∶1091.
2. Paul R,Alistair C,Ahmed J, et al. Development of radioimmunoassays for free tetraLaspartylLlysine trypsinogen activation peptides 〔Ｊ〕. J Immunol Meth,1988； 111(2)∶195.
3. Carlos fernandezdel C,Schmidt J,David W,et al. Generation and possible significance of trypsinogen activation peptide in experimental acute pancreatitis in rat 〔Ｊ〕. Pancreas,1992； 7(3)∶263.
4. Aho HJ, Koskensalo ML, Nevalainen TJ. Experimental pancreatitisin the rat: sodium taurocholateinduced acute haemorrhagic pancreatitis 〔Ｊ〕. Scand J Gastroenterol, 1980； 15(4)∶411.
5. Schmidt J, Lewandrowsi K,Warshaw AL, et al. Morphometric characteristics and homogeneity of a new model of acute pancreatitis in the rat 〔Ｊ〕. Int J Pancreatol,1992； 12(1)∶41.
6. Pozsar J, Berger Z, Simon K, et al. Biphasic effect of prostaglandin E1 on the severity of acute pancreatitis induced by a closed duodenal loop in rats 〔Ｊ〕. Pancreas, 1996； 12(2)∶159.
7. Wang Youxue,Satoru N,Motoji K, et al. Do plasma and urine trypsinogen activation peptides(TAP) really increase in trypsintaurocholateinduced pancreatitis 〔Ｊ〕. Pancreas, 2000； 20(4)∶389.
8. Yasuyuki Nakae, Satoru Naruse, Motoji Kitagawa, et al. Activation of trypsinogen in experimental model of acute pancreatitis in rats 〔Ｊ〕. Pancreas, 1995； 10(3)∶306.
9. Kai M, Casitillo CF,Frick TW, et al. Increased intrapancreatic trypsinogen activation in ischemiainduced experimental pancreatitis 〔Ｊ〕. Ann Surg, 1995； 221(4)∶364.
10. Simpson KW,Bvmts N,Beechey N, et al. Cholecystokinin8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation 〔Ｊ〕. Dig Dis Sci, 1995； 40(10)∶2152.

《中國普外基礎與臨床雜志》

胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產生及意義

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《中國普外基礎與臨床雜志》

胰蛋白酶原激活肽在大鼠實驗性急性胰腺炎中的產生及意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料