DMBA誘導大鼠乳腺癌與纖維肉瘤中cyclin D1及p53蛋白的表達及其意義_《中國普外基礎與臨床雜志》

作者：

張毅 ¹ , 姜軍 ¹ , 韓曉蓉 ¹ , 劉麗梅 ²

1.第三軍醫大學西南醫院普外科(重慶400038);;
2.第三軍醫大學西南醫院病理科;

關鍵詞：

p53蛋白 cyclin D1 乳腺癌纖維肉瘤

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目的探討cyclin D1和p53 蛋白在實驗性大鼠同時性乳腺癌與纖維肉瘤中的表達及其意義。方法通過改良給藥的方法復制大鼠同時性乳腺癌與纖維肉瘤動物模型,應用SP免疫組化技術檢測cyclin D1和p53蛋白在乳腺癌、纖維肉瘤及瘤旁組織中的表達。結果在正常乳腺組織中無cyclin D1和p53蛋白表達，在不典型增生乳腺組織中7/14有cyclin D1表達，8/18的乳腺癌與9/14的纖維肉瘤中有cyclin D1的表達,7/18的乳腺癌與5/14的纖維肉瘤中有p53蛋白的過度表達; 肉瘤旁組織中無cyclin D1和p53蛋白表達。cyclin D1和p53蛋白表達與乳腺癌和纖維肉瘤組織學分級有關，同時二者的表達呈負相關。結論在大鼠同時性乳腺癌與纖維肉瘤中有cyclin D1和p53蛋白過度表達，cyclin D1蛋白可能參與腫瘤發生過程，而p53蛋白表達與腫瘤的惡性程度相關。

引用本文： 張毅,姜軍,韓曉蓉,劉麗梅. DMBA誘導大鼠乳腺癌與纖維肉瘤中cyclin D1及p53蛋白的表達及其意義. 中國普外基礎與臨床雜志, 2001, 8(5): 285-287. doi: 復制

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12.	Mansukhani MM,Osborne BM,Zhong JZ,et al.The pattern of p53 and p21waf1/cip1 immunoreactivity in nonHodgkins lymphomas predicts p53 gene status 〔Ｊ〕. Diag Mol Patho, 1997; 6(4)∶222.
13.	Worsley SD, Jenmngs BA, Khalil, KH, et al. Cyclin D1 amplification and expression in human breast carcinoma: correlation with histological prognostic markers and oestrogen receptor expression 〔Ｊ〕. J Clin Pathol, 1996; 49(1)∶46.

1. 華長江,殷廣福,許正昌.胃腸道多原發癌的及時診斷和治療〔Ｊ〕.實用癌癥雜志,1994; 9(2)∶115.
2. 姜軍,詹新恩,陳意生等.多原發性惡性腫瘤模型的建立〔Ｊ〕. 第三軍醫大學學報,1996; 18(4)∶323.
3. 高進主編.腫瘤學基礎與實驗〔M〕. 第1版. 北京: 人民衛生出版社,1999∶410~411.
4. Russell WO, Cohen J, Enzinger F, et al. A clinical and pathological staging system for soft tissue sarcomas 〔Ｊ〕. Cancer, 1977; 40(7)∶1562.
5. Bito T,Ueda M,Ahmed NU,et al.Cyclin D and retinoblastoma gene product expression in actinic deratosis and cutanious squamous cell carcinoma in relation to p53 expression 〔Ｊ〕. J Cutan Pathol,1998; 22(3)∶427.
6. Lee CC,Yananoto S,Morimura K,et al. Significance of cyclin D1 overexpression in transitional cell carcinomas of the urinary bladder and its correlation with histopathologic features 〔Ｊ〕. Cancer, 1997; 79(4)∶789.
7. Zhang SY,Caamano J,Cooper F,et al.Immunohistochemistry of cyclin D1 in human breast cancer 〔Ｊ〕. Am J Clin Pathol,1994; 102(5)∶695.
8. Naitoh H,Shibata J,Kawaguchi A,et al.Overexpression and localization of cyclin D1 mRNA and antigen in esophageal cancer 〔Ｊ〕. Am J Pathol, 1999; 146(1)∶161.
9. Nishida N,Fukuda Y,Komeda T,et al.Amplification and overexpression of the cyclin D1 gene in aggressive human hepatocellular carcinoma 〔Ｊ〕. Cancer Res,1994; 54(14)∶3107.
10. Betticher DC, Heighway J, Hasleton PS,et al. Prognostic significance of CCND1(cyclin D1) overexpression in primary resected nonsmallcell lung cancer 〔Ｊ〕. Br J Cancer, 1996; 73(4)∶294.
11. Giannino DS,Murphy M,Ruaro EM,et al. Cyclin D1 and p21/war1 are both involved in p53 growth suppression 〔Ｊ〕 . Oncogene 1998; 12(2)∶177.
12. Mansukhani MM,Osborne BM,Zhong JZ,et al.The pattern of p53 and p21waf1/cip1 immunoreactivity in nonHodgkins lymphomas predicts p53 gene status 〔Ｊ〕. Diag Mol Patho, 1997; 6(4)∶222.
13. Worsley SD, Jenmngs BA, Khalil, KH, et al. Cyclin D1 amplification and expression in human breast carcinoma: correlation with histological prognostic markers and oestrogen receptor expression 〔Ｊ〕. J Clin Pathol, 1996; 49(1)∶46.

《中國普外基礎與臨床雜志》

DMBA誘導大鼠乳腺癌與纖維肉瘤中cyclin D1及p53蛋白的表達及其意義

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《中國普外基礎與臨床雜志》

DMBA誘導大鼠乳腺癌與纖維肉瘤中cyclin D1及p53蛋白的表達及其意義

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料