缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用_《中國普外基礎與臨床雜志》

作者：

涂兵 , 嚴律南 , 劉智敏

四川大學華西醫院普外科（成都610041）;

關鍵詞：

缺血預處理大鼠移植肝臟缺血再灌注損傷

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目的探討缺血預處理（ischemic preconditioning,IP）對大鼠移植肝臟缺血再灌注損傷的保護作用。方法采用SD大鼠原位肝移植動物模型，供肝冷保存時間100 min，無肝期25 min。64只SD大鼠隨機均分成兩組：對照組，獲取供肝前僅以肝素生理鹽水經門靜脈灌注； IP組，獲取供肝前阻斷肝門血供10 min，再灌注10 min，然后再以肝素生理鹽水經門靜脈灌注。每組受體的一半（n=8）用于觀察存活率，另一半(n=8)用于移植肝臟再灌注2 h后取血及肝臟檢測。結果IP組的1 w存活率、膽汁分泌量、抗氧化酶活力、血清NO水平均明顯高于對照組(P＜0.05)，血清ALT、AST、LDH、TNF及肝組織中的過氧化產物含量均明顯低于對照組(P＜0.05)，組織的病理改變也輕于對照組。結論IP能夠提高血清NO水平，降低血清TNF含量，對大鼠移植肝臟的缺血再灌注損傷具有保護作用。

引用本文： 涂兵,嚴律南,劉智敏. 缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用. 中國普外基礎與臨床雜志, 2002, 9(2): 89-92. doi: 復制

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10. Bautista AP,Spitzer JJ. Inhibition of nitric oxide formation in vivo enhances superoxide release by the perfused liver \[J\].Am J Physiol,1994; 266(5 Pt 1)∶G783.

《中國普外基礎與臨床雜志》

缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用

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《中國普外基礎與臨床雜志》

缺血預處理對大鼠移植肝臟缺血再灌注損傷的保護作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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