膽囊切除術對二甲基肼誘發小鼠大腸癌的影響_《中國普外基礎與臨床雜志》

作者：

楊波 ¹ , 蔣彥永 ² , 蕭蔭祺 ³ , 蒲永東 ¹

1.解放軍309醫院普外科（北京100091）;;
2.解放軍總醫院普外科（北京100853）;;
3.解放軍309醫院肝膽外科;

關鍵詞：

膽囊切除術大腸癌細胞周期大腸粘膜上皮膽汁酸

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目的探討膽囊切除術對大腸癌發生的影響及作用機理。
方法膽囊切除術后以二甲基肼誘發小鼠大腸癌，觀察大腸腫瘤發生情況；流式細胞術測定大腸粘膜上皮細胞S期百分比；高效液相色譜儀檢測手術前后鼠糞膽酸、鵝脫氧膽酸、脫氧膽酸和石膽酸含量。
結果實驗組荷結節鼠大腸平均荷結節數、受檢鼠大腸腺瘤發生率及S期百分比均明顯高于對照組（P＜0.05）；膽囊切除術后鼠糞石膽酸含量較術前和對照組明顯增加（P=0.00）。
結論膽囊切除后二甲基肼誘發的小鼠大腸腫瘤發生數量有所增加，并且大腸粘膜上皮細胞增殖旺盛，提示膽囊切除術對二甲基肼誘發小鼠大腸腫瘤有協同促進作用，可能與術后腸道內次級膽汁酸石膽酸含量增加有關。

引用本文： 楊波,蔣彥永,蕭蔭祺,蒲永東. 膽囊切除術對二甲基肼誘發小鼠大腸癌的影響. 中國普外基礎與臨床雜志, 2002, 9(1): 19-22. doi: 復制

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12.	Nielsen GP, Theldors A, Tulinius H, et al. Cholecystectomy and colorectal carcinoma: a totalpopulation historical prospective study [Ｊ]. Am J Gastroenterol, 1991; 86(10)∶1486.
13.	Ekbom A, Yuen J, Adami HO, et al. Cholecystectomy and colorectal cancer [Ｊ]. Gastroenterology, 1993; 105(1)∶142.
14.	Narisaw T,Magadia NE,Weisburger JH,et al.Promoting effect of bile acids on colon carcinogenesis after intrarectal instillation of NmethylNnitroNnitnosoguanidine in rats [Ｊ]. J Natl Cancer Inst,1974; 53(4)∶1093.
15.	Reddy BS, Narasswa J, Wesburger JH, et al. Brief communication: promoting effect of sodium deoxycholate on colon adenocarcinomas in germ free rats [Ｊ]. J Natl Cancer Inst,1976; 56(2)∶441.
16.	Zusman I, Zimber A. Ultrastructural changes in rat colorectal epithelium and tumors after treatment with NmethylNnitroNnitrosoguanidine and secondary bile acids [Ｊ]. Acta Anat, 1991; 141(1)∶282.
17.	Morvay K,Szentleleki K,Torok G,et al. Effect of change of fecal bile acid excretion achieved by operative procedures on 1,2dimethylhydrazineinduced colon cancer in rats [Ｊ]. Dis Colon Rectum,1989; 32(10)∶860.
18.	Malagelada JR, Go VLW, Summerskill WHJ, et al. Bile acid secretion and biliary bile acid composition altered by cholecystectomy [Ｊ]. Dig Dis Sci, 1973; 18(5)∶455.
19.	Roda E, Aldini R, Mazzella G, et al. Enteroheptic circulating of bile acids after cholecystectomy [Ｊ]. Gut, 1978; 19(5)∶640.
20.	Zuccato E, Ventari M, Leo GD, et al. Role of bile acids and metabolic activity of colonic bacteria in increased risk of colon cancer after cholecystectomy [Ｊ]. Dig Dis Sci, 1993; 38(3)∶514.
21.	Narisawa T, Sano M, Suto M, et al. The correlation between cholecystectomy and fecal bile acids, and large bowel cancer induced with 1,2dimethylhydrazine in mice [Ｊ]. Dis Colon Rectum, 1985; 28(1)∶27.

1. Alley PG, Lee SP. The increased risk of proximal colonic cancer after cholecystectomy [Ｊ]. Dis Colon Rectum， 1983; 26(8)∶522.
2. Adami HO, Krusemo UB, Meirik O. Unaltered risk of colorectal cancer within 14-17 years of cholecystectomy: updating of a populationbased cohort study [Ｊ]. Br J Surg， 1987; 74(8)∶675.
3. 許紅兵，蕭蔭琪，錢志平,等. 膽囊切除術與大腸癌關系的探討 [Ｊ].中國肛腸病雜志，1989； 9(2)∶25.
4. Lingeman CH, Garner FM. Comparative study of intestinal adenocarcinomas of animals and man [Ｊ]. J Natl Cancer Inst， 1972; 48(2)∶325.
5. Roges AE. Contribution of laboratory animal studies of colon carcinogenesis. In: Masteromarino AJ. Large bowel cancer [M]. New York： Praeger Published, 1985∶ 1-33.
6. Kuniyasu T, Tanaka T, Shima H,et al. Enhancing effect of cholecystectomy on colon carcinogenesis induced by methylazoxymethanol acetate in hamsters [Ｊ]. Dis Colon Rectum, 1986; 29(8)∶492.
7. Fazio VW. Current therapy in colon and rectal surgery [M]. Philadelphia: B.C. Decker Incorporated, 1990∶ 227.
8. PrestonMartin S, Pike MC, Ross RK, et al. Increased cell division as a cause of human cancer [Ｊ]. Cancer Res, 1990; 50(12)∶7415.
9. Vernick LJ,Kuller LH.Cholecystectomy and right side colon cancer: an epidemiological study [Ｊ].Lancet, 1981; 2(8243)∶381.
10. Markman M. Cholecystectomy and carcinoma of the colon [Ｊ]. Lancet, 1982; 2(8288)∶47.
11. Turunen MJ, Kivilaakso EO. Increased risk of colorectal cancer after cholecystectomy [Ｊ]. Ann Surg, 1981; 194(5)∶639.
12. Nielsen GP, Theldors A, Tulinius H, et al. Cholecystectomy and colorectal carcinoma: a totalpopulation historical prospective study [Ｊ]. Am J Gastroenterol, 1991; 86(10)∶1486.
13. Ekbom A, Yuen J, Adami HO, et al. Cholecystectomy and colorectal cancer [Ｊ]. Gastroenterology, 1993; 105(1)∶142.
14. Narisaw T,Magadia NE,Weisburger JH,et al.Promoting effect of bile acids on colon carcinogenesis after intrarectal instillation of NmethylNnitroNnitnosoguanidine in rats [Ｊ]. J Natl Cancer Inst,1974; 53(4)∶1093.
15. Reddy BS, Narasswa J, Wesburger JH, et al. Brief communication: promoting effect of sodium deoxycholate on colon adenocarcinomas in germ free rats [Ｊ]. J Natl Cancer Inst,1976; 56(2)∶441.
16. Zusman I, Zimber A. Ultrastructural changes in rat colorectal epithelium and tumors after treatment with NmethylNnitroNnitrosoguanidine and secondary bile acids [Ｊ]. Acta Anat, 1991; 141(1)∶282.
17. Morvay K,Szentleleki K,Torok G,et al. Effect of change of fecal bile acid excretion achieved by operative procedures on 1,2dimethylhydrazineinduced colon cancer in rats [Ｊ]. Dis Colon Rectum,1989; 32(10)∶860.
18. Malagelada JR, Go VLW, Summerskill WHJ, et al. Bile acid secretion and biliary bile acid composition altered by cholecystectomy [Ｊ]. Dig Dis Sci, 1973; 18(5)∶455.
19. Roda E, Aldini R, Mazzella G, et al. Enteroheptic circulating of bile acids after cholecystectomy [Ｊ]. Gut, 1978; 19(5)∶640.
20. Zuccato E, Ventari M, Leo GD, et al. Role of bile acids and metabolic activity of colonic bacteria in increased risk of colon cancer after cholecystectomy [Ｊ]. Dig Dis Sci, 1993; 38(3)∶514.
21. Narisawa T, Sano M, Suto M, et al. The correlation between cholecystectomy and fecal bile acids, and large bowel cancer induced with 1,2dimethylhydrazine in mice [Ｊ]. Dis Colon Rectum, 1985; 28(1)∶27.

《中國普外基礎與臨床雜志》

膽囊切除術對二甲基肼誘發小鼠大腸癌的影響

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《中國普外基礎與臨床雜志》

膽囊切除術對二甲基肼誘發小鼠大腸癌的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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